Free Radical Scavenging Activity and Total Polyphenol Content of Securidaca Longipedunculata Roots and Leaves Extracts

Abstract

Securidaca longipedunculataFresen. is a widely used medicinal plant in most of the African countries. This paper aims to compare the polyphenolic composition and antioxidant activity of some extracts obtained from S. longipedunculata roots and leaves in different solvents: ethanol (E), petroleum ether (PE), ethyl acetate (EtOAc), and water (W). Polyphenols were analysed by spectrophotometric and chromatographic methods. Evaluation of antioxidant activity was performed by several in vitro methods (DPPH bleaching, FRAP, nitrite-induced auto-oxidation of haemoglobin, inhibition of lipid peroxidation catalysed by cytochrome C). Our results revealed that the leaves were abundant in phenolic principles than the roots and the EtOAc was the appropriate solvent for their extraction. The EtOAc extracts exhibited the best antioxidant potential. Several polyphenols were determined in the leaves extracts for the first time: hyperoside, isoquercitrin, rutin, p-coumaric acid. RezumatSecuridaca longipedunculata Fresen. este o plantă medicinală utilizată pe scară largă în majoritatea țărilor africane. Această lucrare își propune să compare compoziția polifenolică și activitatea antioxidantă a unor extracte obținute din rădăcini și frunze de S. longipedunculata în diferiți solvenți: etanol (E), eter de petrol (PE), acetat de etil (EtOAc) și apă (W). Polifenolii au fost analizați prin metode spectrofotometrice și cromatografice. Evaluarea activității antioxidante a fost efectuată prin mai multe metode in vitro (DPPH, FRAP, autooxidarea hemoglobinei indusă de nitriți, inhibarea peroxidării lipidice catalizată de citocromul c). Rezultatele noastre au arătat că frunzele au fost mai bogate în principii fenolice decât rădăcinile, iar acetatul de etil a fost cel mai bun solvent pentru extracție. Extractele în EtOAc au prezentat cel mai bun potențial antioxidant. Polifenolii de tip hiperozidă, izoquercitrină, rutozidă, acid p-cumaric au fost determinați pentru prima dată în extractele obținute din frunze.

Stachys Species: Comparative Evaluation of Phenolic Profile and Antimicrobial and Antioxidant Potential

“…They were mixed with cytochrome c (2.0 µM) and Stachys extract (8.3 µg/mL). The reaction was monitored at 235 nm [60].…”

Polyphenolic Profile, Anti-Inflammatory and Anti-Nociceptive Activities of Some African Medicinal Plants

“…The column temperature was set at 48 °C. ChemStation and DataAnalysis software (from Agilent, USA) were used for processing the obtained data [ 32 , 33 ]. The compounds were identified by comparison of their retention times (Rt) and the MS spectra with those of the corresponding references.…”

“…The compounds were identified by comparison of their retention times (Rt) and the MS spectra with those of the corresponding references. An external standard method was used for the quantification of the polyphenols based on calibration curves in the 0.5–50 μg/mL range with good linearity (R 2 > 0.999) [ 32 , 33 ].…”

The Investigation of Antimicrobial Activity of Some Extracts From Momordica Charantia by Using as Solvent Extraction an Ionic Liquid

“…The number of studies on the antimicrobial activity of certain classes of compounds has grown continuously over the years. The main objective of the research is to find new antimicrobial agents using plant-based resources to obtain extracts with a robust antimicrobial character [3,4,6]. In recent years, research has been focused on using ionic liquids as green solvents from the newest generation, in the extraction of the active principles from vegetal sources, that present besides the therapeutic importance and a rigorously demonstrated antimicrobial action, have been studied [7,8].…”