Free radical scavenging activities of Cnidium officinale Makino and Ligusticum chuanxiong Hort. methanolic extracts

Abstract

Background:Antioxidants from natural resources possess multifaceted and importance of the activities provides substantial scope in neurodegenerative diseases. The aim of this study was to assess and compare the free radical scavenging activities of Cnidium officinale and Ligusticum chuanxiong, which are closely related species.Materials and Methods:The scavenging activities of plant materials were evaluated using Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbance capacity (ORAC) and 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radical (O2·-), hydrogen peroxide (H2O2), hydroxyl (OH·), nitric oxide radical (NO·) and metal chelation. In addition, the cell viability and nitric oxide release were assayed using Neuro-2a (N2a) cells.Results:The methanolic extracts of C. officinale and L. chuanxiong showed scavenging activities of free radicals with an additional antioxidant capacity. Moreover, the efficacy on the cell viability and nitric oxide release in cell culture model has been established.Conclusion:Results of the present study suggests that the extracts of C. officinale and L. chuanxiong have comparatively similar free radical scavenging activities in vitro and may have important health effects.

Advances in the Chemical Analysis and Biological Activities of Chuanxiong

“…Senkyu, the dried rhizome of Cnidium officinale Makino (syn. L. officinale Kitagawa, Umbelliferae) which is the original plant of the important crude drug CX in China, is one of the most frequently occurring drugs in the prescriptions of traditional Chinese medicines (TCMs) used in Japan [ 7 , 8 , 9 , 10 ]. According to study, C. officinale and L. chuanxiong are closely related species with 98% sequence identity [ 11 ].…”

“…It is one of the major clinically used cardiovascular protective TCMs and a popular medicine widely used in prescriptions for the treatment of atherosclerosis [ 22 ], vasodilatation [ 23 ], thrombus formation [ 24 ], ischemic stroke [ 25 , 26 ], angina pectoris [ 27 ], and hypertension [ 28 , 29 ], because of its reputation for thousands of years in China of facilitating blood circulation and dispersing blood stasis [ 30 , 31 ]. Additionally, it also has antioxidant [ 7 , 14 ], neuroprotective [ 32 , 33 ], antiinflammatory [ 34 ], antibacterial [ 35 ], antiproliferative [ 36 ], and proapoptotic activities [ 37 ]. What is more, its medicinal value has been demonstrated by numerous experiments, pre-clinical studies and clinical trials [ 33 , 35 ].…”

Evaluation of phytochemical composition and antioxidant capacity of a decoction containing Adenanthera pavonina L. and Thespesia populnea L.

“…The decoction used in the present study contains strong nitric oxide scavenging activity with an EC 50 of 14.02 ± 0.66 μg/ml as compared with Pyrus boissieriana , Cnidium officinale , and Ligusticum chuanxiong , which showed an EC50 of 650, 57.25, and 76.50 μg/ml, respectively. [2728] This suggests that the decoction investigated in this study might be a potent and novel therapeutic agent for scavenging of NO and thereby minimizing of pathological conditions caused by excessive generation of NO.…”

Characterization of thrombin/factor Xa inhibitors in Rhizoma Chuanxiong through UPLC-MS-based multivariate statistical analysis

“…Its major chemical components include phthalide lactones, alkaloids, phenolic acids and other constituents [ 15 ]. A large body of studies has shown that Chuanxiong possesses multifarious pharmacological effects, including protective effects on neuron [ 16 ], heart [ 17 ], liver [ 18 ] and kidney [ 19 ], as well as antioxidation [ 20 , 21 ], anti-inflammation [ 22 – 24 ], etc. In our previous studies, Chuanxiong extracts had inhibitory effects on platelet aggregation [ 25 ] and THR activity [ 12 ], while there are few studies on its effects on THR and FXa so far.…”