Free Radical Quenching and Immunomodulatory Effect of a Modified Siddha Preparation, Kalpaamruthaa

Abstract

Kalpaamruthaa (KA), a modified indigenous Siddha formulation constitutes Semecarpus anacardium nut milk extract (SA), Phyllanthus emblica and honey. The present study attempt has been to investigate the antioxidant and immunomodulatory activities of KA. Antioxidant activity of KA was determined by using ferric thiocyanate and thiobarbituric acid methods. KA showed higher antioxidant activity when compared with vitamin E (α-tochoperol), a well-known antioxidant. Immunomodulatory activities on humoral and cellular immunity were studied by heamagglutination (HA) titre, delayed type hypersensitivity (DTH) and phagocytic index. KA was administered at the dosage level of 100, 150, 200, 250 and 300 mg/kg body weight. Also, KA enhanced the HA titre, phagocytic index and delayed type hypersensitivity in a dose dependent manner, which indicates that KA triggers both humoral and cell mediated immune responses to a greater extent. This improved antioxidant and immunomodulatory activities of KA might be due to the synergistic effect of flavonoids, vitamin C and hydrolysable tannins present in the drug. The current study shows that KA is effective at the dosage level of 200 mg/kg body weight were significantly induced immunomodulatory activity in a dose dependant manner.

An Experimental Study to Evaluate the Anti-inflammatory and Immunmodulatory Effects of UNIM-352, a Polyherbal Preparation for Bronchial asthma

“…The IgG mediated formation of antigen-antibody complexes on the surface of mast cells and basophils results in the release of inflammatory mediators of anaphylaxis such as histamine, leukotrienes and prostaglandins (Postma, 1995;Agarwal et al, 1999;Dahanukar et al, 2002;Shanmugam et al, 2007). The effect of UNIM-352 on the degranulation of peritoneal mast cells in sensitized animals was studied and the results showed that UNIM-352 significantly inhibited antigen challenge induced degranulation of mast cells in a dose related manner and also protected the rats against mortality.…”

“…The thickness of the footpad was measured at 24 h after the challenge using a plethysmometer and data acquisition system (Ugo Basile, Italy). The difference in the thickness of the right hind paw and the left hind paw was used as a measure of DTH and the percentage response was evaluated after all treatments ( Shanmugam et al, 2007).…”

Immunomodulatory Potential of Gemmomodified Extract of Terminalia arjuna

“…One of the main strategies of herbal treatment is to increase the body's natural resistance to disease/stress causing agents rather than directly neutralizing the agents itself in practice. Plant origin immunomodulatory agents enhanced para immunity, non specific immunomodulation of granulocytes, macrophages, natural killer cells and complement functions [1][2][3][4][5][6] . Immune diseases are big problem for mankind.…”