Free radical production by femtosecond laser lens irradiation in porcine eyes

Masuda, Yoichiro; Igarashi, Tsutomu; Oki, Kotaro; Kobayashi, Maika; Takahashi, Hiroshi; Nakano, Tadashi

doi:10.1016/j.jcrs.2019.02.035

Cited by 6 publications

(7 citation statements)

References 9 publications

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“…The ex vivo results suggested there were no further increases in the MDA and SOD levels, and hence, this higher density setting could be safely applied in such cases without unwanted increases in free radical formation. This was likely because the total energy generated from the low-energy FSL system was <1-5 J, which was substantially lower compared to that used in the study by Masuda et al [14]. Moreover, with low energy, high pulses, and overlapping spots, there can be no further free radical formation beyond a certain threshold since free radicals cannot be generated in gas and can only occur when interacting with tissue.…”

Section: Discussionmentioning

confidence: 80%

“…However, the association between FLACS and aqueous oxidative stress has not been extensively investigated. In 2019, Masuda et al [ 14 ] showed that lens irradiation with a high-energy FSL platform (CATALYS, Johnson & Johnson Surgical Vision, Inc. Santa Ana, CA, USA) increased the production of aqueous free radicals in proportion to the amount of laser energy, indicating that FLACS can be associated with the increased generation of intracameral free radicals. The authors also suggested that excessive laser irradiation during FLACS might cause increased corneal endothelial damage caused by the free radicals [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…In 2019, Masuda et al [ 14 ] showed that lens irradiation with a high-energy FSL platform (CATALYS, Johnson & Johnson Surgical Vision, Inc. Santa Ana, CA, USA) increased the production of aqueous free radicals in proportion to the amount of laser energy, indicating that FLACS can be associated with the increased generation of intracameral free radicals. The authors also suggested that excessive laser irradiation during FLACS might cause increased corneal endothelial damage caused by the free radicals [ 14 ]. However, this was an ex vivo study in porcine eyes, and it did not take into consideration free radical formation created by the phacoemulsification itself.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Different Capsulotomy and Fragmentation Energy Levels on the Generation of Oxidative Stress Following Femtosecond Laser-Assisted Cataract Surgery

Han,

Liu,

Setiawan

et al. 2024

Biomolecules

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Purpose. This study was conducted to evaluate the effects of different capsulotomy and fragmentation energy levels on the production of oxidative free radicals following femtosecond laser-assisted cataract surgery (FLACS) with a low-energy platform. Methods. The experimental study included 60 porcine eyes (12 groups). In each group, capsulotomies with 90% or 150% energy, and fragmentations with 90%, 100%, or 150% energy or 150% with high spot density, respectively, were performed. Control samples were obtained from non-lasered eyes at the beginning (five eyes) and end (five eyes) of the experiment. In the clinical study, 104 eyes were divided into 5 groups, and they received conventional phacoemulsification (20 eyes), FLACS with 90% capsulotomy and 100% fragmentation energy levels without NSAIDs (16 eyes), FLACS with 90% (26 eyes) or 150% (22 eyes) capsulotomy energy levels, respectively, with a 100% fragmentation energy level and NSAIDs, and FLACS with 90% capsulotomy and 150% fragmentation energy levels and NSAIDs (20 eyes). Aqueous samples were analyzed for their malondialdehyde (MDA) and superoxide dismutase (SOD) levels. Results. In the experimental study, there were no significant differences in the MDA and SOD levels between the groups with different capsulotomy energy levels. An increase in the fragmentation energy from 100% to 150% led to significantly higher MDA levels in the groups with both 90% (p = 0.04) and 150% capsulotomy energy levels (p = 0.03), respectively. However, increased laser spot densities did not result in significant changes in MDA or SOD levels. In the clinical study, all four of the FLACS groups showed higher MDA levels than the conventional group. Similarly, the increase in the fragmentation energy from 100% to 150% resulted in significantly elevated levels of MDA and SOD, respectively. Conclusions. Although increasing the FSL capsulotomy energy level may not have increased free radicals, higher fragmentation energy levels increased the generation of aqueous free radicals. However, fragmentation with high spot density did not generate additional oxidative stress. Increased spot density did not generate additional oxidative stress, and this can be helpful for dense cataracts.

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Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Different Capsulotomy and Fragmentation Energy Levels on the Generation of Oxidative Stress Following Femtosecond Laser-Assisted Cataract Surgery

Han,

Liu,

Setiawan

et al. 2024

Biomolecules

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“…Нирка -один із паренхіматозних органів, який найчутливіший до дії лазерного опромінення [6]. Фотони лазерного опромінення здатні поглинатися специфічними рецепторами -хромофорами, що призведе до змін в біологічних тканинах із ініціацією вільнорадикальних процесів у клітинах, серед яких найпоширеніші -пероксидне окиснення ліпідів (ПОЛ) та окиснювальна модифікація протеїнів (ОМП) [9].…”

Section: інтенсивність вільнорадикальних процесів у постнуклеарній фракції нирок щурів за дії лазерного опромінення та ω-3 поліненасичениunclassified

Intensity of Free Radical Processes in the Postnuclear Fraction of Rat Kidneys Under Influence of Laser Irradiation and Ω-3 Polyunsaturated Fatty Acids

Ketsa¹,

Марченко²,

Samuliak³

2021

Вісник ОНУ

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Introduction. The effect of laser irradiation on the body can lead to local tissue damage, which will be accompanied by changes in biochemical processes that occur in the kidneys. The direction of these changes is completely unclear, so the study of free radical oxidation of lipids and proteins as the main markers of oxidative stress in the body and their correction by ω-3 polyunsaturated fatty acids (PUFA) remains relevant. Aim. To evaluate the intensity of lipid peroxidation and oxidative modification of proteins in the postnuclear fraction of rat kidneys under the action of laser irradiation and additional administration of ω-3 PUFA. Methods. The studies used white outbred rats, which were irradiated daily for 4 minutes with a laser diode with a wavelength of 650 nm, power of 50 mW in the abdominal cavity at a distance of 10 cm from the skin surface. The state of lipid peroxidation (LPO) was determined by the level of primary, secondary and final products. The intensity of oxidative modification of proteins (OMP) was evaluated by the level of carbonyl derivatives and protein SH-groups. To correct the prooxidant state, the animals were additionally injected with ω-3 PUFA. Results. Low-intensity laser irradiation has a destructive effect on the cell membranes of the kidneys, which is expressed by an increase in primary, secondary and final products of LPO in phospholipid extracts and an increase in the intensity of OMP. The introduction of ω-3 PUFAs reduces free radical processes in the kidneys of irradiated rats, but this effect depends on the scheme of their introduction. ω-3 PUFAs do not show antioxidant effect under the conditions of their introduction after laser irradiation. Daily administration of ω-3 PUFA two hours before irradiation shows a slight antioxidant effect only in the initial stages of irradiation. Preliminary seven-day administration of ω-3 PUFA before laser irradiation is the most effective, as it helps to reduce free radical processes. Conclusion. It is established that ω-3 PUFAs are able to have a corrective effect on the action of low-intensity laser irradiation, which depends on the scheme of their introduction. The highest antioxidant effect is observed in groups of animals to which ω-3 PUFA was previously administered before the action of laser irradiation.

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“…FLACS is considered to be easier and more reproducible than conventional cataract surgery 1 . However, some complications were reported particular in FLACS, such as capsule tag, anterior tear 2 , endothelial damage 3 and capsular blockage syndrome during hydrodissection 4 , which were considered as being due to the uniqueness of FLACS.…”

Section: Introductionmentioning

confidence: 99%

Inspection of the Lens Thickness With Preoperative Biometric Measurements Prevents an Erroneous Interpretation of Posterior Capsule During FLACS

Kurosawa

Horiguchi

Shiba³

et al. 2020

Preprint

Self Cite

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Purpose: Optical opacity reduces quality of biometry images, making it potentially difficult to find the correct location for irradiation during femtosecond laser-assisted cataract surgery (FLACS). After experiencing a case of posterior capsule (PC) rupture because of optical opacity, we started lens thickness (LT) inspection, which indicates comparison of between intra- and pre-operatively measured LT. We retrospectively investigated the effectiveness of the LT inspection. Methods: One observer reviewed all FLACS treatment summaries for 3 years by CATALYS® in the Jikei University Hospital, Tokyo. Based on the lines defining the PC on intraoperative OCT images, all cases were classified into three groups: undescribed, appropriate and inappropriate PC. Results: Among the 1070 cases, 1047 cases had appropriate PC. In 19 cases, the PC line was undescribed because of dense cataract. Among 474 cases with no inspection, 4 cases had an inappropriate PC. Whereas, in 596 cases with the LT inspection, there was no case of an inappropriate PC. LT inspection significantly reduced the cases with inappropriate PC.Conclusions: The safety margins normally work to prevent severe complications. However, rare outlier cases had a high risk of severe complications. We propose LT inspection could be the most practical and convenient way for safety surgery.

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Free radical production by femtosecond laser lens irradiation in porcine eyes

Cited by 6 publications

References 9 publications

Effects of Different Capsulotomy and Fragmentation Energy Levels on the Generation of Oxidative Stress Following Femtosecond Laser-Assisted Cataract Surgery

Effects of Different Capsulotomy and Fragmentation Energy Levels on the Generation of Oxidative Stress Following Femtosecond Laser-Assisted Cataract Surgery

Intensity of Free Radical Processes in the Postnuclear Fraction of Rat Kidneys Under Influence of Laser Irradiation and Ω-3 Polyunsaturated Fatty Acids

Inspection of the Lens Thickness With Preoperative Biometric Measurements Prevents an Erroneous Interpretation of Posterior Capsule During FLACS

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