Free-Radical Oxidation and Antioxidants

Dormandy, T. L.

doi:10.1016/s0140-6736(78)91148-0

Cited by 211 publications

(78 citation statements)

References 28 publications

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“…Transferrin is normally present in serum, and the amount of FBS and AHS in both modified KCM formulations was speculated to provide a sufficient amount of transferrin, with no noticeable deficient effects in culture using the modified KCM. Additionally, serum also contains various antioxidants (Dormandy, 1978), which may compensate for the effects of excluding selenium. To prepare modified KCM for the culture of human epithelial cells for clinical applications, autologous serum is a useful alternative to FBS.…”

Section: Discussionmentioning

confidence: 99%

Preparation of keratinocyte culture medium for the clinical applications of regenerative medicine

Takagi

Yamato

Murakami

et al. 2010

J Tissue Eng Regen Med

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Keratinocyte culture medium (KCM) has been used for the in vitro culture of keratinocytes and other types of epithelial cells, and the medium includes various ingredients. In this study, two modified KCMs were prepared. In the first, insulin, hydrocortisone and antibiotics that are normally included in KCM were replaced with clinically approved pharmaceutical agents, except transferrin and selenium; in the second, cholera toxin (CT) was replaced by L-isoproterenol (ISO). The modified KCMs were then compared to conventional KCM containing laboratory-grade reagents. Induced cell colony formations of canine oral mucosal epithelial cells cultured in both modified KCMs were found to be nearly equivalent to that in the control KCM, and there was no significant difference between the effect of CT and ISO. Canine oral mucosal cells proliferated to confluence in all three KCM formulations, with or without the use of 3T3 feeder layers. Cultured epithelial cells were harvested from temperature-responsive culture surfaces as an intact cell sheet, and the immunohistochemical analysis of the sheets showed that p63 and cytokeratin were expressed in the epithelial cell sheets cultured in all KCMs. Eventually, in the modified KCM formula, fetal bovine serum was replaced by autologous human serum, and the formula was found to be able to fabricate human oral mucosal epithelial cell sheets. These results indicated that the modified KCM was equally efficient as conventional KCM in the fabrication of transplantable stratified epithelial cell sheets.

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Section: Discussionmentioning

confidence: 99%

Preparation of keratinocyte culture medium for the clinical applications of regenerative medicine

Takagi

Yamato

Murakami

et al. 2010

J Tissue Eng Regen Med

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“…Our results suggest that one of the major functions of serum in culture is the function as an antioxidant and that substance which causes the antioxidant activity is different from vitamin E. Indeed, the antioxidant activity of serum has been proved to be the function of two proteins; one was apotransferrin and the other was ceruloplasmin (Dormandy 1978;Granfield et al 1979). Therefore, it will be necessary to examine the effect of these two proteins as antioxidants in our culture system.…”

Section: Discussionmentioning

confidence: 71%

Oxygen-dependent growth declining and effect of vitamin E for human diploid fibroblasts in serum-free, BSA-containing culture.

Kan

Yamane

1983

Tohoku J. Exp. Med.

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Growth promoting activity of bovine serum albumin for human diploid fibroblasts was affected by oxygen environment. When cells (2 x 104 cells/35 mm dish) were cultured with BSA of 5 g/liter supplemented RITC 80-7 under hypoxic (7%) environment, excellent growth was maintained during the period of culture, while the growth under aerobic environment markedly declined after 3 days of culture. The culture challenged with BSA under aerobic environment generated a relatively large amount of malondialdehyde (MDA) as a product of lipid peroxidation.Vitamin E restored the declining of growth and inhibited MDA formation.Also in vitro lifespan of the cells challenged with BSA markedly decreased under aerobic environment as compared with that of the cells under hypoxic environment.Vitamin E did not restore the oxygen-dependent decreasing of in vitro lifespan. On the other hand, the cells cultured with serum supplemented medium grew and phased out similarly under both oxygen environment as well as the case with BSA supplemented RITC 80-7 under hypoxic environment.These results suggest that the extent of cell proliferation is controlled in part by lipid proxidation and serum antioxidant activity is not identical from that of vitamin E. -bovine serum albumin; oxygen environment; lipid peroxidation; vitamin E; cell proliferation

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“…However, the brain is one organ that is, at first instance, susceptible to peroxidase damage ( Julka et al, 1992). Lead can alter the cellular redox state by inhibiting the enzymes involved in antioxidant defense, that is catalase, glutathione peroxide, glutathione and superoxide dismutase, which function as blockers of free radical process (Dormandy, 1978). Most of these enzymes are metalloenzymes, w sulfhydryl groups which are essential for their activities, and the oxidation of these groups that are essential for their activities, and the oxidation of these groups by lead may result in partial or complete inhibition of antioxidant defense system.…”

Section: Discussionmentioning

confidence: 99%

Lead Toxicity and The Hypothalamic-Pituitary-Testicular Axis

Hamadouche¹,

Nesrine²,

Abdelkeder³

2013

Not Sci Biol

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Environmental exposure to toxic levels of lead (Pb) occurs in a number of industries with potential adverse effects on the reproductive capacity of exposed men. Clinical and animal studies indicate that abnormalities of spermatogenesis result from toxic lead exposure, but eventual histopathologic alterations involved have not been identified. To explore putative abnormalities in the reproductive gonadotropic axis following lead intoxication, experimental animals when exposed to low levels of lead, 65 days old animals were treated with distilled water containing 0,0 mg (control), 10 mg lead (Pb)/Kg/day and 15 mg lead (Pb)/Kg/day intraperitoneally for 20 days. At the end of treatment, the animals were sacrificed and the blood collected for luteinizing hormone (LH) and testosterone assays. The testis was processed for histological analysis. The results showed a high serum concentration of LH and testosterone in lead-treated animals compared to controls. Histological examination of testis showed deformities in testicular morphology of lead intoxicated animals with gross damage within the somniferous tubules. A strong correlation was established between LH and testosterone suggesting an alteration in the endocrine components of the gonadotropic axis. Histological examination of pituitary gland showed some degenerative changes in endocrine cells of lead group. Changes in LH and testosterone levels suggest that Pb exposure during the critical time of sexual differentiation induces reproductive axis abnormalities in adulthood. In conclusion, lead has a gonadotoxic effect by decreasing LH and testosterone levels and damaging the testis seminiferous tubules. Catalase activity was significantly reduced in the lead group following 65 days of exposure which possibly indicates that lead might had other mechanisms of action, such as increasing oxidative damage.

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Free-Radical Oxidation and Antioxidants

Cited by 211 publications

References 28 publications

Preparation of keratinocyte culture medium for the clinical applications of regenerative medicine

Preparation of keratinocyte culture medium for the clinical applications of regenerative medicine

Oxygen-dependent growth declining and effect of vitamin E for human diploid fibroblasts in serum-free, BSA-containing culture.

Lead Toxicity and The Hypothalamic-Pituitary-Testicular Axis

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