Free Radical-Mediated Effects on Skeletal Muscle Protein in Rats Treated with Fe-Nitrilotriacetate

Nagasawa, Takashi; Hatayama, Takahiro; Watanabe, Yoshiaki; Tanaka, Miho; Niisato, Yushi; Kitts, David D.

doi:10.1006/bbrc.1996.6034

Cited by 49 publications

(24 citation statements)

References 27 publications

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“…2A, B, i.e., both force production and protein concentrations were progressively decreased under hypoxicfatigue conditions. Although we have not yet been able to specifically identify all these proteins, it should be noted that our results are in agreement with those reported by other laboratories [26,27,28,29]. These groups have respectively demonstrated that hypoxemia, ischemia and ROS overload (caused by iron injection) are all able to induce protein changes by oxidation, proteolysis and degradation.…”

Section: Discussionsupporting

confidence: 91%

“…Band 55 kDa was decreased only in hypoxic-fatigue conditions, suggesting specificity of protein degradation instead of a more general degradation pattern under hypoxic/no-fatigue and hypoxicfatigue conditions. Nagasawa et al [28] have described myosin and actin modifications after ferric nitrilotriacetate (Fe-NTA) injection into rats. These authors also observed a 66-kDa band that was strongly detected by anti-dinitrophenylhydrazone (anti-DNP).…”

Section: Discussionmentioning

confidence: 99%

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Hypoxia and fatigue-induced modification of function and proteins in intact and skinned murine diaphragm muscle

Brotto

Leyen

Nosek

et al. 2000

Pflugers Arch - Eur J Physiol

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Fatigue studies of isolated, intact muscles typically utilize solutions saturated with O2. However, under in vivo fatiguing conditions, less oxygen is delivered to the muscles and they actually experience hypoxia. No studies to date have correlated the effects of acute hypoxia on the isometric contractile properties of intact muscles, skinned fibers isolated from the same muscles, and the cellular content of specific muscle proteins. Therefore, we have studied the effects of in vitro acute hypoxia on the fatigability of intact diaphragm muscle strips and on the isometric contractile properties of single Triton-skinned fibers isolated from control and hypoxic diaphragm muscles. We found that hypoxia and fatiguing stimulation per se affect the tetanic force of intact muscle strips without exhibiting any significant deleterious effects on the calcium-activated force of skinned muscle fibers dissected from the intact muscles. In contrast, fatiguing stimulation under hypoxic conditions decreased both the tetanic force of muscle strips and the calcium-activated force of skinned muscle fibers. Gel electrophoresis of muscles subjected to hypoxia and hypoxic-fatigue revealed that there is a significant reduction in three protein bands when compared to control muscles. Protein modification may be the underlying mechanism of muscle fatigue under physiologic conditions.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Hypoxia and fatigue-induced modification of function and proteins in intact and skinned murine diaphragm muscle

Brotto

Leyen

Nosek

et al. 2000

Pflugers Arch - Eur J Physiol

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“…An important point is that oxidative stress has been reported to significantly modify the conformation and increase the degradation of actin and myosin in rat skeletal muscle [113]. These alterations may decrease the troponic-C affinity for calcium, so that a given increase in calcium in the vicinity of the troponin complex will have less of an activating effect on muscle contraction.…”

Section: Myofilamentsmentioning

confidence: 99%

From muscle disuse to myopathy in COPD: potential contribution of oxidative stress

Couillard

Préfaut²

2005

European Respiratory Journal

164

125

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Evidence has been accumulating that chronic inactivity leading to muscle disuse is unlikely to be the only explanation for the peripheral muscle dysfunction of chronic obstructive pulmonary disease (COPD) patients.Although a new concept of myopathy was recently proposed, the question of disuse and/or a form of myopathy is still being debated. This review proposes definitions for the terms used in this debate, discusses the relevant studies and concludes that the evidence points to a myopathy associated with muscle disuse in COPD.COPD myopathy implies pharmacological and/or pathophysiological mechanisms that need to be identified in order to optimally orient therapeutic strategies. The literature indicates that corticosteroids, inflammation, hypoxaemia and oxidative stress are among the factors contributing to COPD muscle dysfunction, but their relative contributions have not been fully elucidated.This review presents the advances in understanding each of these mechanisms, especially the data showing that muscle oxidative stress occurs and contributes to muscle dysfunction in chronic obstructive pulmonary disease. The current review also reports the studies that have elucidated the molecular mechanisms underlying this stress in chronic obstructive pulmonary disease by demonstrating alterations in oxidant and/or antioxidant systems. Finally, the review considers how inflammation and hypoxaemia may trigger oxidative stress in chronic obstructive pulmonary disease muscles and presents the therapeutic modalities that should be proposed to prevent it.

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“…Therefore the free radicals may act the occurrence of muscular dystrophy (37). Skeletal muscle proteins could be damaged (38) or modified (39) by oxidative stress. We reported that the serum gluco corticoid concentration increased in rats that were ex posed to suspension hypokinesia (40).…”

Section: Resultsmentioning

confidence: 99%

Effect of Different Dietary Protein Composition on Skeletal Muscle Atrophy by Suspension Hypokinesia/Hypodynamia in Rats.

Tada

Yokogoshi

2002

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Free Radical-Mediated Effects on Skeletal Muscle Protein in Rats Treated with Fe-Nitrilotriacetate

Cited by 49 publications

References 27 publications

Hypoxia and fatigue-induced modification of function and proteins in intact and skinned murine diaphragm muscle

Hypoxia and fatigue-induced modification of function and proteins in intact and skinned murine diaphragm muscle

From muscle disuse to myopathy in COPD: potential contribution of oxidative stress

Effect of Different Dietary Protein Composition on Skeletal Muscle Atrophy by Suspension Hypokinesia/Hypodynamia in Rats.

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