Free Radical Lipid Peroxidation in the Nuclei of Mouse Liver Cells under Normal Conditions and after Partial Hepatectomy

Abstract

The dynamics and periodicity of LPO processes in the nuclei and chromatin were detected during regenerative processes in mouse liver. LPO processes were enhanced in transcriptionally active and replicating chromatin. Phosphatidylcholine, phosphatidylinositol, esterified cholesterol, and free fatty acids were maximally oxidized during active transcription, and cardiolipin was oxidized during active replication. These processes were accompanied by periodical changes in activity of antioxidant enzymes. High activity of SOD during transcriptional activity of liver cells was typical for nuclear matrix-attached chromatin, but this parameter decreased during replication. Catalase activity significantly increased in the fractions of high salt chromatin and nuclear matrix-bound chromatin during replication.

The Study of Modifications of Genomic DNA in Endotoxemia

“…Earlier we have shown that under conditions of increased activity of lipid peroxidation, cells respond by modification of chromatin lipids, coupled with changes in the amount of separate lipids and degree of their oxidation [25].…”