Free-radical damage by UV or hypoxanthine-xanthine oxidase in cultured human skin fibroblasts: Protective effects of two human plasma fractions

Masini, V.; Noël-Hudson, Marie-Sophie; Wépierre, J.

doi:10.1016/0887-2333(94)90188-0

Cited by 3 publications

(3 citation statements)

References 37 publications

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“…Similar to nonmelanoma skin cancer, melanoma also undergoes a multistage development towards the fully malignant phenotype. A failure of proper detoxification of ROS by antioxidant enzymes results in an increase of the load of ROS that had been shown to be involved in all three stages of carcinogenesis (Cerutti et al, 1994), and is produced by both the UVA and the UVB components of sunlight (Masini et al, 1994). As well as causing permanent genetic changes involving protooncogenes and tumor suppressor genes, ROS activate cytoplasmic signal transduction pathways that are related to growth, differentiation, senescence, and tissue degradation (Cerutti, 1994;Brenneisen et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…As to the regulation of the induced synthesis and activity of MnSOD upon UVA irradiation, it is most likely that O 2 •and IL-1α and IL-1β, both released following UVA irradiation, qualify to mediate UVA effects (Kupper et al, 1987;Masini et al, 1994;Wlaschek et al, 1994). At least, both O 2 •and IL-1α and IL-1β are well-known stimuli of the MnSOD (Marklund, 1992;Akashi et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

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Adaptive Antioxidant Response of Manganese-Superoxide Dismutase Following Repetitive UVA Irradiation

Poswig

Wenk

Brenneisen

et al. 1999

Journal of Investigative Dermatology

108

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In response to the attack of reactive oxygen species, the skin has developed a complex antioxidant defense system including among others the manganese-superoxide dismutase (MnSOD). MnSOD dismutates the superoxide anion (O2*-) derived from the reduction of molecular oxygen to hydrogen peroxide (H2O2), which is detoxified by glutathione peroxidase to water and molecular oxygen. We have addressed the question whether MnSOD is inducible upon UVA irradiation and whether repetitive UV exposure, as practiced for the light-hardening during phototherapy of various photodermatoses, can even enhance the adaptive antioxidant response. Single exposure of four different strains of fibroblasts to UVA irradiation resulted in a dose- and time-dependent increase in specific MnSOD mRNA levels. Interestingly, repetitive UVA exposure at days 1, 2, and 3 at a dose rate of 200 kJ per m2 resulted in a 5-fold induction of specific MnSOD mRNA levels following the third UVA exposure. Similar results were obtained for MnSOD activity. This adaptive response in terms of upregulation of the antioxidant enzyme MnSOD correlates with the protection against high UV doses, if cells were preexposed to sublethal UV doses. Importantly, MnSOD substantially differed between the tested individuals in both mRNA and activity levels. Taken together, we here provide evidence for the increasing induction of MnSOD upon repetitive UVA irradiation that may contribute to the effective adaptive UVA response of the skin during light hardening in phototherapy. Interindividual differences in the inducibility of MnSOD might account for differences in the susceptibility to develop photodermatologic disorders related to photosensitivity, photoaging, and skin cancer. The molecular basis for interindividual differences in the inducibility of antioxidant enzymes remains to be elucidated.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Adaptive Antioxidant Response of Manganese-Superoxide Dismutase Following Repetitive UVA Irradiation

Poswig

Wenk

Brenneisen

et al. 1999

Journal of Investigative Dermatology

108

View full text Add to dashboard Cite

show abstract

“…36,37 In fact, IL-1␣, IL-1␤, and TNF-␣ are well-known stimuli of the MnSOD. 16,38 In addition, our data show that neutralizing antibodies against IL-1␣, IL-1␤, and TNF-␣ can significantly suppress the induction of MnSOD activity in fibroblasts after transfer of supernatants from previously UV-B-irradiated epidermal cells.…”

Section: Commentmentioning

confidence: 99%

Induction of Manganese Superoxide Dismutase in Human Dermal Fibroblasts

Naderi-Hachtroudi¹,

Peters²,

Brenneisen³

et al. 2002

Arch Dermatol

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Immunochemical quantitation of UV-induced oxidative and dimeric DNA damage to human keratinocytes

Cooke

Mistry

Ladapo

et al. 2000

Free Radical Research

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There is growing evidence to suggest that solar radiation-induced, oxidative DNA damage may play an important role in skin carcinogenesis. Numerous methods have been developed to sensitively quantitate 8-oxo-2'deoxyguanosine (8-oxodG), a recognised biomarker of oxidative DNA damage. Immunoassays may represent a means by which the limitations of many techniques, principally derived from DNA extraction and sample workup, may be overcome. We report the evaluation of probes to thymine dimers and oxidative damage in UV-irradiated cells and the DNA derived therefrom. Thymine dimers were most readily recognised, irrespective of whether in situ in cells or in extracted DNA. However, using antibody-based detection the more subtle oxidative modifications required extraction and, in the case of 8-oxodG, denaturation of the DNA prior to successful recognition. In contrast, a recently described novel probe for 8-oxodG detection showed strong recognition in cells, although appearing unsuitable for use with extracted DNA. The probes were subsequently applied to examine the relative induction of lesions in cells following UV irradiation. Guanine-glyoxal lesions predominated over thymine dimers subsequent to UVB irradiation, whereas whilst oxidative lesions increased significantly following UVA irradiation, no induction of thymine dimers was seen. These data support the emerging importance of oxidative DNA damage in UV-induced carcinogenesis.

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Free-radical damage by UV or hypoxanthine-xanthine oxidase in cultured human skin fibroblasts: Protective effects of two human plasma fractions

Cited by 3 publications

References 37 publications

Adaptive Antioxidant Response of Manganese-Superoxide Dismutase Following Repetitive UVA Irradiation

Adaptive Antioxidant Response of Manganese-Superoxide Dismutase Following Repetitive UVA Irradiation

Induction of Manganese Superoxide Dismutase in Human Dermal Fibroblasts

Immunochemical quantitation of UV-induced oxidative and dimeric DNA damage to human keratinocytes

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