Free-radical chain oxidation of rat red blood cells by molecular oxygen and its inhibition by α-tocopherol

Miki, Masayuki; Tamai, Hiroshi; Mino, Makoto; Yamamoto, Yorihiro; Niki, Etsuo

doi:10.1016/0003-9861(87)90358-4

Cited by 343 publications

(195 citation statements)

References 30 publications

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“…The oxidation of cellular components can cause apoptosis [118]. It has been thought that the HMWPs are formed by the cross-linking of LMWPs and oxidized lipids in membranes [119,120]. In the present study, Ala, Cit and Pro markedly prevented the • OH-induced formation of HMWPs and decrease of LMWPs in carp erythrocytes.…”

Section: Effects Of Ala Cit and Pro On • Oh-induced Apoptosissupporting

confidence: 52%

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Jiang

Liu

et al. 2016

Free Radical Biology and Medicine

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a b s t r a c tThe present study explored the apoptosis pathways in hydroxyl radicals ( • OH)-induced carp erythrocytes. Carp erythrocytes were treated with the caspase inhibitors in physiological carp saline (PCS) or Ca 2 þ -free PCS in the presence of 40 μM FeSO 4 /20 μM H 2 O 2 . The results showed that the generation of reactive oxygen species (ROS), the release of cytochrome c and DNA fragmentation were caspase-dependent, and Ca 2 þ was involved in calpain activation and phosphatidylserine (PS) exposure in • OH-induced carp erythrocytes. Moreover, the results suggested that caspases were involved in PS exposure, and Ca 2 þ was involved in DNA fragmentation in • OH-induced fish erythrocytes. These results demonstrated that there might be two apoptosis pathways in fish erythrocytes, one is the caspase and cytochrome c-dependent apoptosis that is similar to that in mammal nucleated cells, the other is the Ca 2 þ -involved apoptosis that was similar to that in mammal non-nucleated erythrocytes. So, fish erythrocytes may be used as a model for studying oxidative stress and apoptosis in mammal cells. Furthermore, the present study investigated the effects of glutamine (Gln)'s metabolites [alanine (Ala), citrulline (Cit), proline (Pro) and their combination (Ala 10 Pro 4 Cit 1 )] on the pathways of apoptosis in fish erythrocytes. The results displayed that Ala, Cit, Pro and Ala 10 Pro 4 Cit 1 effectively suppressed ROS generation, cytochrome c release, activation of caspase-3, caspase-8 and caspase-9 at the physiological concentrations, prevented Ca 2 þ influx, calpain activation, PS exposure, DNA fragmentation and the degradation of the cytoskeleton and oxidation of membrane and hemoglobin (Hb) and increased activity of anti-hydroxyl radical (AHR) in • OH-induced carp erythrocytes. Ala 10 Pro 4 Cit 1 produced a synergistic effect of inhibited oxidative stress and apoptosis in fish erythrocytes. These results demonstrated that Ala, Cit, Pro and their combination can protect mammal erythrocytes and nucleated cells against oxidative stress and apoptosis. The studies supported the use of Gln, Ala, Cit and Pro as oxidative stress and apoptosis inhibitors in mammal cells and the hypothesis that the inhibited effects of Gln on oxidative stress and apoptosis are at least partly dependent on that of its metabolites in mammalian.

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Section: Effects Of Ala Cit and Pro On • Oh-induced Apoptosissupporting

confidence: 52%

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Jiang

Liu

et al. 2016

Free Radical Biology and Medicine

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“…The antioxidant activity of the leaves' methanolic extracts was measured as the inhibition of erythrocyte hemolysis (Miki, Tamia, Mino, Yamamoto, & Niki, 1987). Blood was obtained from a male ram of body weight $67 kg.…”

Section: Assay For Erythrocyte Hemolysis Mediated By Peroxyl Free Radmentioning

confidence: 99%

Antioxidant activity and phenolic contents of Olea europaea L. leaves sprayed with different copper formulations

et al. 2007

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Olive trees (Olea europaea L. Cv. Cobrançosa) from the northeast of Portugal were sprayed with three different copper formulations [bordeaux mixture (copper sulphate + calcium hydroxide À 20% Cu), copper hydroxide (40% Cu) and copper oxychloride (50% Cu)] to control olive fungal diseases. The residues of copper in olive leaves, harvested at different times, were evaluated by atomic absorption spectrometry. At all the collection times, treated olive leaves had significantly higher copper contents, compared to the control. The different copper amounts in pesticide formulations lowered the leaves contents in total phenols and hence their antioxidant properties. Olive leaves sprayed with copper oxychloride possessed the highest copper levels and the lowest content in phenols, which influenced its antioxidant activity (higher EC 50 values for reducing power, scavenging effect on DPPH radicals and inhibition of erythrocyte hemolysis). Leaves without copper residues proved to be a good natural source of antioxidants, giving values comparable to the reference compounds.

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“…The antioxidant activity of the methanol solutions from compounds was measured as the inhibition of erythrocyte hemolysis. 21 Blood was obtained from male ram (churra galega transmontana) of body weight $67 kg. Erythrocytes separated from the plasma and the buffy coat were washed three times with 10 mL of 10 mM phosphate-buffered-saline (PBS; 10 mM, 10 mL) at pH 7.4, prepared by mixing NaH 2-PO 4 and Na 2 HPO4 (10 mM) and NaCl (125 mM) in 1 L of distilled water, and centrifuged at 1500g for 5 min.…”

Section: 23mentioning

confidence: 99%

Synthesis and antioxidant activity evaluation of new 7-aryl or 7-heteroarylamino-2,3-dimethylbenzo[b]thiophenes obtained by Buchwald–Hartwig C–N cross-coupling

Queiroz

Ferreira

Calhelha

et al. 2007

Bioorganic & Medicinal Chemistry

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Abstract-New 7-aryl or 7-heteroarylamino-2,3-dimethylbenzo [b]thiophenes were prepared by palladium-catalyzed Buchwald-Hartwig cross-coupling of 7-bromo or 7-amino-2,3-dimethylbenzo[b]thiophenes, previously prepared by us, with substituted (4-methoxy or 3,4-dimethoxy) anilines and 3-aminopyridine or with substituted (3-methoxy or 4-cyano) bromobenzenes and 2-bromopyridine, respectively, using Pd(OAc) 2 , rac-BINAP or Xantphos as ligands, and Cs 2 CO 3 as base. Their antioxidant properties were evaluated by several methods: reducing power, scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, inhibition of erythrocyte hemolysis and inhibition of lipid peroxidation using the b-carotene linoleate system. EC 50 values for all the methods were determined and it was possible to establish some structure-activity relationships (SARs) based on the presence and position of different substituents on the phenyl ring (1 or 2 OMe and C"N), on the presence of a pyridine ring and on the position of its nitrogen atom relative to the N-H bond.

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Free-radical chain oxidation of rat red blood cells by molecular oxygen and its inhibition by α-tocopherol

Cited by 343 publications

References 30 publications

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Antioxidant activity and phenolic contents of Olea europaea L. leaves sprayed with different copper formulations

Synthesis and antioxidant activity evaluation of new 7-aryl or 7-heteroarylamino-2,3-dimethylbenzo[b]thiophenes obtained by Buchwald–Hartwig C–N cross-coupling

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