Free-living Amoebae Promote Growth and Survival of Helicobacter pylori

Winiecka-Krusnell, Jadwiga; Wreiber, Karin; Euler, Ann von; Engstrand, Lars; Linder, Ewert

doi:10.1080/00365540110080052

Cited by 97 publications

(85 citation statements)

References 4 publications

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“…Amoebae feed on mixed communities of bacteria within biofilms including, pathogenic bacteria such as species of Legionella, Mycobacterium, P. aeruginosa, Vibrio cholerae, Helicobacter pylori [6,12,45,46,59]. Once inside the amoebal cell, some bacteria will survive or escape the adverse conditions presented by digestive vacuoles, but can also find sanctuary from unfavourable environmental conditions and can multiply [25].…”

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confidence: 99%

Risk Assessment for the Spread of Serratia marcescens Within Dental-Unit Waterline Systems Using Vermamoeba vermiformis

et al. 2015

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(2015) Risk assessment for the spread of Serratia marcescens within dental unit waterline systems using Vermamoeba vermiformis. Current Microbiology, 71 (4). pp. 434 442.It is advisable to refer to the publisher's version if you intend to cite from the work.http://dx.doi.org/10.1007/s00284-015-0872-0For more information about UCLan's research in this area go to http://www.uclan.ac.uk/researchgroups/ and search for . Conflict of interest disclosure statementAll named authors declare that there is no conflict of interest.2 Title: Risk assessment for the spread of Serratia marcescens within dental-unit waterline systems using Vermamoeba vermiformis Abstract:Vermamoeba vermiformis is associated with the biofilm ecology of dental-unit waterlines (DUWLs). This study investigated whether V. vermiformis is able to act as a vector for potentially pathogenic bacteria and so aid their dispersal within DUWL systems. Clinical dental water was initially examined for Legionella species by inoculating it onto Legionella selective-medium plates. The molecular identity/profile of the glassy colonies obtained indicated none of these isolates were Legionella species. During this work bacterial colonies were identified as a non-pigmented Serratia marcescens. As the water was from a clinical DUWL which had been treated with Alpron™ this prompted the question as to whether S. marcescens had developed resistance to the biocide. Exposure to Alpron™ indicated that this dental biocide was effective, under laboratory conditions, against S. marcescens at up to 1x10 8 colony forming units/millilitre (cfu/ml). V. vermiformis was cultured for eight weeks on cells of S. marcescens and Escherichia coli. Subsequent electron microscopy showed that V.vermiformis grew equally well on S. marcescens and E. coli (p = 0.0001). Failure to detect the presence of S.marcescens within the encysted amoebae suggests that V. vermiformis is unlikely to act as a vector supporting the growth of this newly isolated, nosocomial bacterium.

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Risk Assessment for the Spread of Serratia marcescens Within Dental-Unit Waterline Systems Using Vermamoeba vermiformis

et al. 2015

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“…It has also been suggested that H. pylori may survive in water distribution networks by becoming associated with autochthonous microorganisms present in biofilms formed in such systems (5,18,29). Park et al (22) reported the detection of H. pylori for the first time in drinking water biofilms taken from a public water distribution system.…”

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confidence: 99%

Shear Stress, Temperature, and Inoculation Concentration Influence the Adhesion of Water-Stressed Helicobacter pylori to Stainless Steel 304 and Polypropylene

Azevedo

Pinto

Reis

et al. 2006

Appl Environ Microbiol

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Although molecular techniques have identified Helicobacter pylori in drinking water-associated biofilms, there is a lack of studies reporting what factors affect the attachment of the bacterium to plumbing materials. Therefore, the adhesion of H. pylori suspended in distilled water to stainless steel 304 (SS304) coupons placed on tissue culture plates subjected to different environmental conditions was monitored. The extent of adhesion was evaluated for different water exposure times, using epifluorescence microscopy to count total cell numbers. High shear stresses-estimated through computational fluid dynamics-negatively influenced the adhesion of H. pylori to the substrata (P < 0.001), a result that was confirmed in similar experiments with polypropylene (P < 0.05). However, the temperature and inoculation concentration appeared to have no effect on adhesion (P > 0.05). After 2 hours, H. pylori cells appeared to be isolated on the surface of SS304 and were able to form small aggregates with longer exposure times. However, the formation of a three-dimensional structure was only very rarely observed. This study suggests that the detection of the pathogen in well water described by other authors can be related to the increased ability of H. pylori to integrate into biofilms under conditions of low shear stress. It will also allow a more rational selection of locations to perform molecular or plate culture analysis for the detection of H. pylori in drinking water-associated biofilms.

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“…There is only one published study about the interactions between H. pylori and amoebae [23]. In this paper, the authors showed that H. pylori was able to survive within trophozoites of Acanthamoeba castellanii under experimental conditions.…”

Section: H Pylorimentioning

confidence: 71%

“…Although further research regarding bacteriaamoeba interactions is necessary, the current knowledge for all bacteria present in the CCL [7,13,23,26,30,41,49] indicates that amoebae could play a key role in bacterial resistance to disinfection, and survival and spread in drinking water systems. Additionally, bacteria-amoeba interactions suggest that, after a contamination episode, the presence of fecal-related pathogens, such as C. jejuni, E. coli O157:H7, H. pylori, S. enterica and S. sonnei, could not be detected using only conventional fecal indicators, as some enteric bacterial pathogens may survive within a moebae and still be present at lower concentrations.…”

Section: Discussionmentioning

confidence: 99%

Amoeba-Related Health Risk in Drinking Water Systems: Could Monitoring of Amoebae Be a Complementary Approach to Current Quality Control Strategies?

Codony¹,

Pérez

Adrados

et al. 2011

Future Microbiol.

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Culture-based methods for fecal indicator microorganisms are the standard protocol to assess potential health risk from drinking water systems. However, these traditional fecal indicators are inappropriate surrogates for disinfection-resistant fecal pathogens and the indigenous pathogens that grow in drinking water systems. There is now a range of molecular-based methods, such as quantitative PCR, which allow detection of a variety of pathogens and alternative indicators. Hence, in addition to targeting total Escherichia coli (i.e., dead and alive) for the detection of fecal pollution, various amoebae may be suitable to indicate the potential presence of pathogenic amoeba-resisting microorganisms, such as Legionellae. Therefore, monitoring amoeba levels by quantitative PCR could be a useful tool for directly and indirectly evaluating health risk and could also be a complementary approach to current microbial quality control strategies for drinking water systems.

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Free-living Amoebae Promote Growth and Survival of Helicobacter pylori

Cited by 97 publications

References 4 publications

Risk Assessment for the Spread of Serratia marcescens Within Dental-Unit Waterline Systems Using Vermamoeba vermiformis

Risk Assessment for the Spread of Serratia marcescens Within Dental-Unit Waterline Systems Using Vermamoeba vermiformis

Shear Stress, Temperature, and Inoculation Concentration Influence the Adhesion of Water-Stressed Helicobacter pylori to Stainless Steel 304 and Polypropylene

Amoeba-Related Health Risk in Drinking Water Systems: Could Monitoring of Amoebae Be a Complementary Approach to Current Quality Control Strategies?

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