Free-living amoebae in sediments from the Lascaux Cave in France

García‐Sánchez, Ángela María; Ariza, C.; Úbeda, J. M.; Martin‐Sanchez, Pedro M.; Jurado, Valme; Bastian, Fabiola; Alabouvette, Claude; Sáiz‐Jiménez, Cesáreo

doi:10.5038/1827-806x.42.1.2

Cited by 18 publications

(9 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reyranella massiliensis , a species close to R. soli , was found on the paintings of an Etruscan tomb (Diaz‐Herraiz et al ., ) and represented 18% of all Alphaproteobacteria detected. The presence of R. massiliensis in subterranean environments could be related to the abundance of amoebae, from which it is an endosymbiont (Pagnier et al ., ), as also found for some other genera, including Legionella in Lascaux Cave (France; Garcia‐Sanchez et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Origin of abundant moonmilk deposits in a subsurface granitic environment

et al. 2018

Self Cite

View full text Add to dashboard Cite

Subsurface granitic environments are scarce and poorly investigated. A multidisciplinary approach was used to characterize the abundant moonmilk deposits and associated microbial communities coating the granite walls of the 16th Century Paranhos spring water tunnel in Porto city (north-west Portugal). It is possible that this study is the first record of moonmilk in an urban subsurface granitic environment. The morphology and texture, mineralogical composition, stable isotope composition and microbial diversity of moonmilk deposits have been studied to infer the processes of moonmilk formation. These whitish secondary mineral deposits are composed of very fine needlefibre calcite crystals with different morphologies and density. Calcified filaments of fungal hyphae or bacteria were distinguished by field emission scanning electron microscopy. Stable isotope analysis revealed a meteoric origin of the needle-fibre calcite, with an important contribution of atmospheric CO 2 , soil respiration and HCO À 3 from weathering of Ca-bearing minerals. The DNAbased analyses revealed the presence of micro-organisms related to urban contamination, including Actinobacteria, mainly represented by Pseudonocardia hispaniensis, Thaumarchaeota and Ascomycota, dominated by Cladosporium. This microbial composition is consistent with groundwater pollution and contamination sources of the overlying urban area, including garages, petrol stations and wastewater pipeline leakage, showing that the Paranhos tunnel is greatly perturbed by anthropogenic activities. Whether the identified microorganisms are involved in the formation of the needle-fibre calcite or not is difficult to demonstrate, but this study evidenced both abiotic and biogenic genesis for the calcite moonmilk in this subsurface granitic environment.

show abstract

Section: Discussionmentioning

confidence: 97%

Origin of abundant moonmilk deposits in a subsurface granitic environment

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although FLA appear to be omnipresent in soils [15,16], few studies are currently available. So far, FLA have been detected in deserts [17], wet soils [18,19], in sediments [20,21] and in soil from agricultural and mining sites [22].…”

Section: Introductionmentioning

confidence: 99%

Cartography of Free-Living Amoebae in Soil in Guadeloupe (French West Indies) Using DNA Metabarcoding

et al. 2020

View full text Add to dashboard Cite

Free-living amoebae (FLA) are ubiquitous protists. Pathogenic FLA such as N. fowleri can be found in hot springs in Guadeloupe, soil being the origin of this contamination. Herein, we analyzed the diversity and distribution of FLA in soil using a targeted metataxonomic analysis. Soil samples (n = 107) were collected from 40 sites. DNA was extracted directly from soil samples or from FLA cultivated at different temperatures (30, 37 and 44 °C). Metabarcoding studies were then conducted through FLA 18SrDNA amplicons sequencing; amplicon sequence variants (ASV) were extracted from each sample and taxonomy assigned against SILVA database using QIIME2 and SHAMAN pipelines. Vermamoeba were detected in DNA extracted directly from the soil, but to detect other FLA an amoebal enrichment step was necessary. V. vermiformis was by far the most represented species of FLA, being detected throughout the islands. Although Naegleria were mainly found in Basse-Terre region, N. fowleri was also detected in Grand Terre and Les Saintes Islands. Acanthamoeba were mainly found in areas where temperature is approx. 30 °C. Vannella and Vahlkampfia were randomly found in Guadeloupe islands. FLA detected in Guadeloupe include both pathogenic genera and genera that can putatively harbor microbial pathogens, therefore posing a potential threat to human health.

show abstract

“…The diversity of protists was studied in mostly aquatic habitats like subterranean streams, lakes and various smaller water pools that formed inside the caves (Vandel 1965;Hoover 1969, 1970). Some data refer to cave sediments and transitional habitats characterized by water layers present on solid surfaces, biofilms and fungal mycelia biomass (Golemansky and Bonnet 1994;Coppellotti and Guidolin 1999;Coppellotti Krupa and Guidolin 2003;Bastian et al 2009;Garcia-Sanchez et al 2013). Epibiotic protists were registered on cave-dwelling animals (Matjašič 1962;Dovgal and Vargovitsh 2010).…”

Section: Introductionmentioning

confidence: 99%

Transitional and small aquatic cave habitats diversification based on protist assemblages in the Veternica cave (Medvednica Mt., Croatia)

Baković¹,

Kepčija²,

Siemensma³

2022

View full text Add to dashboard Cite

Protists in caves are scarcely researched. Most cave studies address the diversity of protists, but very little is known about their habitats and spatio-temporal dynamics. The aim of this study was to investigate the diversity and abundance of protists in the Veternica cave in the Medvednica mountain in Croatia on hygropetric and sinter and clay pools during six months. During this study, 47 protists taxa were distinguished belonging to the groups of heterotrophic flagellates, ciliates, heliozoans, stramenopiles and testate and naked amoebae. The highest taxon richness was found at the sinter pool site richest in bats guano (36 taxa). Most diverse were testate amoebae and ciliates. The number of taxa and their abundance differed significantly between the sampling sites. The prospected habitats supported different protist assemblages, proved by ordination analysis. The most distinctive habitat was the hygropetric. Habitat heterogeneity could be attributed to the presence of inorganic and organic sediments at the sites and habitat microhydrology (lotic or lentic system). Kendall’s concordance coefficient showed a good synchronicity between the habitats in the Veternica cave, based on taxon richness and abundance of protists indicating similar seasonal trends. Seasonality in the studied habitats is attributed to the hydro-meteorological conditions in the Veternica cave drainage area. This study is one of the few studies of spatio-temporal diversity and abundance of protists in caves. Despite the similar appearance of small transitional and aquatic habitats in caves, an example of this study showed specific habitat diversification.

show abstract

Free-living amoebae in sediments from the Lascaux Cave in France

Cited by 18 publications

References 19 publications

Origin of abundant moonmilk deposits in a subsurface granitic environment

Origin of abundant moonmilk deposits in a subsurface granitic environment

Cartography of Free-Living Amoebae in Soil in Guadeloupe (French West Indies) Using DNA Metabarcoding

Transitional and small aquatic cave habitats diversification based on protist assemblages in the Veternica cave (Medvednica Mt., Croatia)

Contact Info

Product

Resources

About

Free-living amoebae in sediments from the Lascaux Cave in France

Cited by 18 publications

References 19 publications

Origin of abundant moonmilk deposits in a subsurface granitic environment

Origin of abundant moonmilk deposits in a subsurface granitic environment

Cartography of Free-Living Amoebae in Soil in Guadeloupe (French West Indies) Using DNA Metabarcoding

﻿Transitional and small aquatic cave habitats diversification based on protist assemblages in the Veternica cave (Medvednica Mt., Croatia)

Contact Info

Product

Resources

About

Transitional and small aquatic cave habitats diversification based on protist assemblages in the Veternica cave (Medvednica Mt., Croatia)