Free living amoebae and human disease

Bursle, Evan; Robson, Jennifer

doi:10.1071/ma16009

Cited by 5 publications

(3 citation statements)

References 35 publications

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“…In this study, Acanthamoeba infection diagnosis and Acanthamoeba keratitis prognosis in non-contact lens wearers were both greatly improved by the use of PCR tests. It allows more rapid diagnosis with small samples, and although it increases the sensitivity diagnostic, requires specialized laboratories 13,14 .…”

Section: Resultsmentioning

confidence: 99%

Isolation and molecular characterization of Acanthamoeba spp. from Iraqi patients with Keratitis

Aldin,

Ardalan,

Jassim

2024

Baghdad Sci.J

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Isolation and molecular characterization of Acanthamoeba spp. related corneal infections in Iraqi Patients. A total of 96 samples from corneal scraps and contact lenses were gathered from corneal patients for the current trial, which runs from November 2020 to May 2021. All plates were checked, classifying them as positive or negative morphologically identification of Acanthamoeba spp. where PCR analysis, which entails the targeted amplification of nucleic acids, has been carried out, and where The 18s rRNA gene's DF3 region was the focus of the isolated strains' sequences. Three patients had Acanthamoeba spp. infections, according to the findings. then the sequence analysis was performed using the NCBI tool, which showed the presence of 2 different genotypes, two strains were grouped into the T3 genotype, one from T4, then they were registered in GeneBank under number accession (MZ427912, MZ349042) respectively. To our knowledge, these are the first reported cases of Acanthamoeba keratitis in Iraq.

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Section: Resultsmentioning

confidence: 99%

Isolation and molecular characterization of Acanthamoeba spp. from Iraqi patients with Keratitis

Aldin,

Ardalan,

Jassim

2024

Baghdad Sci.J

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“…Main findings/outcomes Bastida et al (2016) Omics used for studying the catabolic pathways in Sphingomonadeles aid in compost bioremediation Yoneda et al (2016) To enhance the phenolic tolerance and accumulation of lipid in Rhodoccus opacus transcriptomics has been found quite an effective omics approach Bursle and Robson (2016) Nonculture diagnostic techniques allows detection of organism that are current difficult or impossible to culture Bargiela et al (2015) Metabolome-based techniques can be used to predict microbial activities; to comprehend their functional inputs to environments in which they thrive Krumsiek et al (2015) From such an investigation, it was revealed that metabolomics approach can significantly be applied to monitor the hazardous waste and the consequences associated with it microRNA levels can also be used to investigate the gene expression regulation. MicroRNAs are noncoding RNA molecules regulating the mRNA transcription process and are crucial for a variety of gene expression patterns (Pritchard et al, 2012).…”

Section: Referencementioning

confidence: 99%

Marine bacteria and omic approaches: A novel and potential repository for bioremediation assessment

Hassan

Sabreena

Khurshid

et al. 2022

Journal of Applied Microbiology

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Marine environments accommodating diverse assortments of life constitute a great pool of differentiated natural resources. The cumulative need to remedy unpropitious effects of anthropogenic activities on estuaries and coastal marine ecosystems has propelled the development of effective bioremediation strategies. Marine bacteria producing biosurfactants are promising agents for bio‐remediating oil pollution in marine environments, making them prospective candidates for enhancing oil recovery. Molecular omics technologies are considered an emerging field of research in ecological and diversity assessment owing to their utility in environmental surveillance and bioremediation of polluted sites. A thorough literature review was undertaken to understand the applicability of different omic techniques used for bioremediation assessment using marine bacteria. This review further establishes that for bioremediation of environmental pollutants (i.e. heavy metals, hydrocarbons, xenobiotic and numerous recalcitrant compounds), organisms isolated from marine environments can be better used for their removal. The literature survey shows that omics approaches can provide exemplary knowledge about microbial communities and their role in the bioremediation of environmental pollutants. This review centres on applications of marine bacteria in enhanced bioremediation, using the omics approaches that can be a vital biological contrivance in environmental monitoring to tackle environmental degradation. The paper aims to identify the gaps in investigations involving marine bacteria to help researchers, ecologists and decision‐makers to develop a holistic understanding regarding their utility in bioremediation assessment.

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“…Free-living amoebae (FLA) of the genus Acanthamoeba are cosmopolitan protozoans commonly found in natural environments, they play a significant ecological role in controlling bacterial populations. Some species of this genus, in addition to being ecologically relevant, are also important in the medical field, due to their ability to exist as free-living organisms and as parasites, becoming a threat to the health and life of the hosts [ 1 , 2 ]. In the cornea, Acanthamoeba are etiological agents of Acanthamoeba keratitis (AK), a painful, vision–threatening infection occurring primarily in immunocompetent persons and contact lens users [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Schwann Cell Autophagy and Necrosis as Mechanisms of Cell Death by Acanthamoeba

et al. 2020

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Amoebae of the genus Acanthamoeba are etiological agents of granulomatous amoebic encephalitis (GAE). Recently, through an in vivo GAE model, Acanthamoeba trophozoites were immunolocalized in contact with the peripheral nervous system (PNS) cells—Schwann cells (SC). In this study, we analyzed in greater detail the in vitro early morphological events (1, 2, 3, and 4 h) during the interaction of A. culbertsoni trophozoites (ATCC 30171) with SC from Rattus norvegicus (ATCC CRL-2941). Samples were processed for scanning and transmission electron microscopy as well as confocal microscopy. After 1 h of interaction, amoebae were observed to be adhered to the SC cultures, emitting sucker-like structures associated with micro-phagocytic channels. In addition, evidence of necrosis was identified since edematous organelles as well as multivesicular and multilamellar bodies characteristics of autophagy were detected. At 2 h, trophozoites migrated beneath the SC culture in which necrosis and autophagy persisted. By 3 and 4 h, extensive lytic zones were observed. SC necrosis was confirmed by confocal microscopy. We reported for the first time the induction of autophagic and necrotic processes in PNS cells, associated in part with the contact-dependent pathogenic mechanisms of A. culbertsoni trophozoites.

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Free living amoebae and human disease

Cited by 5 publications

References 35 publications

Isolation and molecular characterization of Acanthamoeba spp. from Iraqi patients with Keratitis

Isolation and molecular characterization of Acanthamoeba spp. from Iraqi patients with Keratitis

Marine bacteria and omic approaches: A novel and potential repository for bioremediation assessment

Schwann Cell Autophagy and Necrosis as Mechanisms of Cell Death by Acanthamoeba

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