Free insulin growth factor-I and vascular endothelial growth factor in the vitreous fluid of patients with proliferative diabetic retinopathy

Simó, Rafael; Lecube, Albert; Segura, Ramón; Arumí, José García; Hernández, Cristina

doi:10.1016/s0002-9394(02)01538-6

Cited by 76 publications

(42 citation statements)

References 47 publications

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“…SRIF levels in the vitreous of patients with diabetic retinopathy are significantly lower than those in nondiabetic control subjects (Hernandez et al, 2005;Simo et al, 2002). This observation suggests that the intravitreous deficit of SRIF may contribute to the process of retinal neovascularisation typical of proliferative retinopathy and supports the concept that adequate levels of SRIF are needed for the maintenance of retinal homeostasis.…”

Section: Diabetic Retinopathysupporting

confidence: 53%

“…Angiogenesis is a complex process, but it may be regarded as the result of changes in the equilibrium between anti-and proangiogenic factors. For instance, the insulin-like growth factor-1 (IGF-1), stimulated by growth hormone, increases in the vitreous of diabetic retinopathy patients (Simo et al, 2002), and it is a major pro-angiogenic factor (DeBosch et al, 2001). SRIF analogues are powerful inhibitors of growth hormone release and, therefore, decrease the blood IGF-1 concentration.…”

Section: Diabetic Retinopathymentioning

confidence: 99%

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Physiology and pathology of somatostatin in the mammalian retina: A current view

Cervia

Casini

Bagnoli

2008

Molecular and Cellular Endocrinology

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. Physiology and pathology of somatostatin in the mammalian retina: A current view. Molecular and Cellular Endocrinology, Elsevier, 2008, 286 (1-2) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Diabetic Retinopathysupporting

confidence: 53%

Section: Diabetic Retinopathymentioning

confidence: 99%

Physiology and pathology of somatostatin in the mammalian retina: A current view

Cervia

Casini

Bagnoli

2008

Molecular and Cellular Endocrinology

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“…Second, the disruption of the bloodretina barrier that occurs in diabetic retinopathy produces an increase in proteins in the vitreous body of diabetic patients. Indeed, we have repeatedly detected three-to fourfold higher levels of intravitreal proteins in diabetic patients than in non-diabetic subjects [2][3][4]. Therefore, an elevated intravitreal level of a particular protein does not necessarily indicate an increase in intraocular production, and might simply reflect a non-specific increase in protein levels due to serum diffusion.…”

Section: Introductionmentioning

confidence: 85%

“…The exclusion criteria were as follows: (1) previous vitreoretinal surgery; (2) photocoagulation in the preceding 6 months; (3) recent vitreous haemorrhage (<3 months before vitrectomy) or intravitreous haemoglobin >5 mg/ml; (4) renal failure (creatinine ≥120 μmol/l); and (5) other chronic diseases apart from diabetes. Details of vitrectomy and sample collection have been described previously elsewhere [3].…”

Section: Methodsmentioning

confidence: 99%

Proteomic analysis of human vitreous fluid by fluorescence-based difference gel electrophoresis (DIGE): a new strategy for identifying potential candidates in the pathogenesis of proliferative diabetic retinopathy

Garcia-Ramírez

Canals

Hernández³

et al. 2007

Diabetologia

147

134

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Aims/hypothesis The aim of this study was to compare the protein profile of vitreous fluid from diabetic patients with proliferative diabetic retinopathy (PDR) with that from nondiabetic patients with idiopathic macular holes (MH). The mRNA of proteins differentially produced was also assessed in the retinas from diabetic and non-diabetic donors. Materials and methods Vitreous humour from type 1 diabetic patients with PDR (n=8) and from non-diabetic patients with MH (n=10) closely matched in terms of age were studied. The comparative proteomic analysis was performed using fluorescence-based difference gel electrophoresis (DIGE). Differentially produced proteins (abundance ratio >1.4, p<0.05) were identified by mass spectrometry. Expressions of mRNA were measured by real-time RT-PCR in retinas from ten human eyes obtained at post-mortem (five eyes from diabetic subjects and five eyes from non-diabetic subjects). Results Eight proteins were highly produced in PDR patients in comparison with non-diabetic subjects: zinc-α 2 -glycoprotein (ZAG), apolipoprotein (apo) A1, apoH, fibrinogen A, and the complement factors C3, C4b, C9 and factor B). We found three proteins that were underproduced in PDR subjects: pigment epithelial derived factor (PEDF), interstitial retinol-binding protein (IRBP) and inter-α-trypsin inhibitor heavy chain (ITIH2). There was no overlap in the vitreous levels of the above-mentioned proteins between PDR patients and non-diabetic control subjects. The differential production of ZAG, C3, factor B, PEDF and IRBP was further confirmed by western blot, and was in agreement with mRNA levels detected in the retina. Conclusions/interpretation Proteomic analysis by DIGE, which permits an accurate quantitative comparison, was useful in identifying new potential candidates involved in the pathogenesis of PDR.

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“…The vitreous provides a means of indirectly exploring the events that are taking place in the retina. Previous reports of vitreous proteins in PDR used the conventional enzyme linked immunosorbent assay (ELISA) method [3-5]. In these studies, however, only a certain set of targeted proteins from vitreous samples were identified because the amount of available vitreous was limited, which makes it difficult to evaluate the changes in the vitreous protein profiles and to identify novel marker proteins of PDR pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of the vitreous proteome in proliferative diabetic retinopathy

Wang

Hu³

et al. 2012

Proteome Sci

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BackgroundDiabetes can lead to serious microvascular complications such as proliferative diabetic retinopathy (PDR), which results in severe vision loss. The diabetes-induced alterations in the vitreous protein composition in diabetic patients with PDR may be responsible for the presence of PDR. The vitreous humour can be utilised in a variety of studies aimed toward the discovery of new targets for the treatment or prevention of PDR and the identification of novel disease mechanisms. The aim of this study was to compare the protein profile of vitreous humour from diabetic patients with PDR with that of vitreous humour from normal human eyes donated for corneal transplant.ResultsVitreous humour from type 2 diabetic patients with PDR (n = 10) and from normal human eyes donated for corneal transplant (n = 10) were studied. The comparative proteomic analysis was performed using two-dimensional fluorescence difference gel electrophoresis (2-D DIGE). Differentially produced proteins (abundance ratio > 2 or < -2, p < 0.01) were identified by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and MALDI-TOF tandem mass spectrometry. A total of 1242 protein spots were detected on the 2-D master gel of the samples, and 57 spots that exhibited statistically significant variations were successfully identified. The spots corresponded to peptide fragments of 29 proteins, including 8 proteins that increased and 21 proteins that decreased in PDR. Excluding the serum proteins from minor vitreous haemorrhage, 19 proteins were found to be differentially produced in PDR patients compared with normal subjects; 6 of these proteins have never been reported to be differentially expressed in PDR vitreous: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (DDAH 1), tubulin alpha-1B chain, gamma-enolase, cytosolic acyl coenzyme A thioester hydrolase, malate dehydrogenase and phosphatidylethanolamine-binding protein 1 (PEBP 1). The differential production of pigment epithelium-derived factor (PEDF) and clusterin was confirmed by Western blot analysis.ConclusionsThese data provide an in-depth analysis of the human vitreous proteome and reveal protein alterations that are possibly involved in the pathogenesis of PDR. Further investigation of these special proteins may provide potential new targets for the treatment or the prevention of PDR.

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Free insulin growth factor-I and vascular endothelial growth factor in the vitreous fluid of patients with proliferative diabetic retinopathy

Cited by 76 publications

References 47 publications

Physiology and pathology of somatostatin in the mammalian retina: A current view

Physiology and pathology of somatostatin in the mammalian retina: A current view

Proteomic analysis of human vitreous fluid by fluorescence-based difference gel electrophoresis (DIGE): a new strategy for identifying potential candidates in the pathogenesis of proliferative diabetic retinopathy

Characterisation of the vitreous proteome in proliferative diabetic retinopathy

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