Free-flow biomolecular concentration and separation of proteins and nucleic acids using teíchophoresis

Doria, Steven; Yost, Jarad; Gagnon, Zachary

doi:10.1016/j.talanta.2022.124198

Cited by 3 publications

(1 citation statement)

References 33 publications

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“…Other recent applications of various electromigration methods in the free-flow instrumental format for middle scale isolation of biomolecules including peptides are reported in recent reviews [291,292]. FFZE implemented in microfluidic devices can also be used for microscale isolation of peptides and other biomolecules [293].…”

Section: Micropreparative Separationsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Section: Micropreparative Separationsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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Teíchophoresis-enabled electrokinetic sample preparation and detection of calcium in natural plant samples

Doria,

Islam,

Gagnon

2024

Talanta

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A Review of Bioanalytical Applications of Microextraction Techniques Combined with Derivatization

Manousi,

Ntorkou,

Tzanavaras

et al. 2023

Bioanalysis

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Microextraction techniques have attracted the attention of many researchers working in the field of bioanalysis due to their unique advantages, mainly in downsizing the scale of sample preparation steps. In parallel, analytical derivatization offers a powerful combination in terms of additional sensitivity, selectivity and compatibility with modern separation techniques. The aim of this review is to discuss the most recent advances in bioanalytical sample preparation based on the combination of microextraction and analytical derivatization. Both innovative fundamental reports and analyte-targeted applications are included and discussed. Dispersive liquid–liquid extraction and solid-phase microextraction are the most common techniques that typically combined with derivatization, while the development of novel and greener protocols is receiving substantial consideration in the field of analytical chemistry.

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Free-flow biomolecular concentration and separation of proteins and nucleic acids using teíchophoresis

Cited by 3 publications

References 33 publications

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Teíchophoresis-enabled electrokinetic sample preparation and detection of calcium in natural plant samples

A Review of Bioanalytical Applications of Microextraction Techniques Combined with Derivatization

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