“…PC presented a high degree of hydrolysis in all performed conditions on the hydrolysis processes. These results can be correlated with the ones described by Penci, 2010. In that work, it was reported that phosphatidylcholine is the phospholipid with higher tendency to be hydrolyzed when using a porcine pancreatic PLA 2 with a very low amount of sunflower lecithins (1 mg).…”
Section: Compositional Analysis Of Modified Lecithinssupporting
“…PC presented a high degree of hydrolysis in all performed conditions on the hydrolysis processes. These results can be correlated with the ones described by Penci, 2010. In that work, it was reported that phosphatidylcholine is the phospholipid with higher tendency to be hydrolyzed when using a porcine pancreatic PLA 2 with a very low amount of sunflower lecithins (1 mg).…”
Section: Compositional Analysis Of Modified Lecithinssupporting
“…Besides of fatty acids, plant oils/fats and animal oils/fats contain triglycerides with the main constituent. Fatty acids are formed during enzymatic hydrolysis of triglyceride especially when the oils are kept in humid atmosphere [22][23][24][25][26][27]. Fatty acid can also be formed during purification of vegetable oils and fats [28][29][30].…”
“…The long-chain nonesterified fatty acids were extracted from the reaction mixture with a two-phase heptane-isopropyl alcohol-water system. The extraction of free fatty acids with the two-phase system was much more simple and convenient than separation by TLC (6,16). The method for determination of the positional specificity reported in the present study is rapid and convenient.…”
Section: Kinetic Propertiesmentioning
confidence: 76%
“…AF-1 isolated from an oil mill was purified and the characteristics of the purified enzyme were studied. Furthermore, we determined the type of phospholipase with a two-phase heptane-isopropyl alcoholwater system which provides sufficient analytical specificity for separation of long-chain non-esterified fatty acids under standard conditions before GC analysis (6,16).…”
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