Free fatty acid metabolic profile and biomarkers of isolated post-challenge diabetes and type 2 diabetes mellitus based on GC–MS and multivariate statistical analysis

Elevated circulating stearic acid leads to a major lipotoxic effect on mouse pancreatic beta cells in hyperlipidaemia via a miR-34a-5p-mediated PERK/p53-dependent pathway

“…No difference was observed in the total SFA content between the HSD and HPD groups, excluding the possibility that the different extent of lipotoxicity was caused by something other than differences in lipid concentrations. Additionally, these results are also consistent with our previous findings [7,31], indicating that stearic acid appears to be a more important risk factor for type 2 diabetes than other NEFA. Therefore, great attention should be paid to elevated serum stearic acid, as it † † † † SA siRNA-NC siRNA-p53 appears to have a greater negative impact on beta cell function.…”

Fasting Serum Lipid and Dehydroepiandrosterone Sulfate as Important Metabolites for Detecting Isolated Postchallenge Diabetes: Serum Metabolomics via Ultra-High-Performance LC-MS

“…The cutoff values for FPG and 2 h-PG used to diagnose IPD were 108 mg/dL (6.0 mmol/L) and 200 mg/dL (11.1 mmol/L), respectively. The patient samples in the current study were different from those used previously (13 ). Enrolled patients were not on any medication before sample collection.…”

“…On the basis of the findings of earlier studies, we hypothesized that considerable metabolic variation exists in the fasting serum glucose concentrations of patients with IPD, supporting the possibility of identifying clinically significant metabolites to facilitate IPD diagnosis. In keeping with this theory, our group recently showed that free fatty acid (FFA) profiles in fasting serum of patients with diabetes are useful for IPD diagnosis in large populations (13 ). Notably, FFA profiles represent only a small proportion of the metabolite fingerprint.…”

The profile of plasma non-esterified fatty acids in children with different terms of type 1 diabetes mellitus

“…Composition and quantitative content of non-esterified fatty acids (NEFA) were investigated in plasma samples of healthy children (12) and children with type 1 diabetes mellitus (DM1) (31) by gas chromatography (GC) after preliminary NEFA solid-phase extraction from plasma lipids. There was a significant (p<0.001) 1.6-fold increase in the total level of NEFA regardless of the disease duration.…”

“…Результаты многочисленных исследований свидетельствуют об увеличении содержания НЭЖК в плазме крови, которое обычно связывают с риском развития сахарного диабета 2 типа [9][10][11][12]. Изменения НЭЖК плазмы крови при развитии сахарного диабета 1 типа (СД1) изучены не столь хорошо [13][14].…”