Free, Conjugated, and Bound Polyamines during the Cell Cycle of Synchronized Cell Suspension Cultures of Nicotiana tabacum

Pfosser, Martin; Königshofer, Helga; Kandeler, Riklef

doi:10.1016/s0176-1617(11)80216-6

Cited by 45 publications

(20 citation statements)

References 19 publications

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“…Developmental processes in plants that are, in part, controlled by cytokinin, may also be affected by PAs, and the promotive effect of cytokinins on endogenous PAs levels, especially on free putrescine, is pronounced (Cho 1983, Hemanataranjan and Vaishampayan 1984, Walker et al 1988. Putrescine is the most abundant PA in cycling cells (Maki et al 1991, Pfosser 1993, Pfosser et al 1990). The precise part a lowering of endogenous PA levels may play in the response to cytokinin starvation has yet to be investigated.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical study of cell cycle control by cytokinin in cultured soybean cells

Mader

Hanke

1996

J Plant Growth Regul

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An immunocytochemical method was used to determine the proportion of cells in the DNA synthesis (S phase) of the mitotic cycle in suspension cultures of soybean (Glycine max (L.) Merr. cv. Acme) callus of cotyledonary origin, the stably cytokinin-dependent tissue used in the cytokinin bioassay devised by Carlos O.Miller. A standard cell synchronization protocol involving hydroxyurea was used to demonstrate the applicability of the immunocytochemical method to this cell culture. Cells were brought to mitotic arrest by cytokinin withdrawal, and the cell division cycle was restarted by the addition of cytokinin. We have followed the pattern of resumption of S phase after the readdition of cytokinin. This pattern reveals the existence of three subpopulations of cells in cytokinin-starved cultures, consistent with the occurrence of three cytokinin-requiring events in the cell cycle: one in mitosis, one in S phase, and one in the G~ phase.

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Section: Discussionmentioning

confidence: 99%

Immunocytochemical study of cell cycle control by cytokinin in cultured soybean cells

Mader

Hanke

1996

J Plant Growth Regul

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“…The acid-soluble fraction of PAs was extracted, benzoylated, and analyzed for free and conjugated PAs as described previously by Pfosser et al (1990) with minor modifications. The benzoylated PAs were extracted in diethyl ether only once but in a larger volume (i.e.…”

Section: Analysis Of Polyamines By Hplcmentioning

confidence: 99%

Polyamine Sparing May Be Involved in the Prolongation of Cell Division Due to Inhibition of Phenylpropanoid Synthesis in Cytokinin-Starved Soybean Cells

Mader

Hanke

1997

J Plant Growth Regul

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Effects on growth, mostly of an inhibitory nature, have been attributed to phenolic compounds in vivo and in vitro. This suggests that L-␣-aminooxy-␤-phenylpropionic acid (L-AOPP), a competitive inhibitor of phenylalanine ammonia-lyase (PAL), the enzyme controlling the first step in phenylpropanoid synthesis, might stimulate growth in soybean suspension cultures (Glycine max, cv. Acme). The promotive effect of L-AOPP, measured as an increase in cell number, was more clearly detected in the growth-limiting condition of cytokinin starvation. At least one more cell division cycle was completed in the presence of L-AOPP before growth by division ceased and growth continued by expansion only. Phenolic acids are known to conjugate with polyamines, modulating the free levels of these plant growth substances. Thus, the effect of L-AOPP on the titers of free and conjugated polyamines (putrescine, spermidine, and spermine) was investigated by high performance liquid chromatography in the course of cytokinin starvation. An increased level of free putrescine was detected in the presence of L-AOPP relative to controls, especially in the initial period before growth became restricted to cell expansion. The decrease in free putrescine associated with the cessation of cell division was temporarily delayed, suggesting that an interaction between phenolic acids and polyamines is involved in the mechanism of growth promotion by L-AOPP.

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“…The endogenous polyamines are known to be essential for normal growth and development and to play several important roles in growth and differentiation through their binding to DNA, RNA, and proteins due to their polycationic nature (Tabor and Tabor, 1984;Kaminska et al, 1990;Pfosser et al, 1990;Kotzabasis and Senger, 1994;Scoccianti et al, 1995). In addition, exogenous polyamines have been shown to stimulate growth and cell division in a wide range of plants (Smith, 1985;Wu and Kuniyuki, 1985).…”

Section: Introductionmentioning

confidence: 99%

Detection of free polyamine in coastal seawater using ion exchange chromatography

Nishibori

2001

ICES Journal of Marine Science

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Polyamines are widely distributed in living organisms and are known to be essential elements for normal growth and development. Although they may affect phytoplankton growth their existence and concentration in seawater is unclear. In this paper the polyamine concentrations in coastal seawater are measured by high performance liquid chromatography (HPLC) using cation exchange resin and OPA reagent. This method seems sufficient for their detection in seawater. Putrescine and spermidine were found to be the major polyamines in the coastal seawater, though their respective concentrations varied widely. In addition to them cadaverine, norspermidine, norspermine, and spermine were also detected. International Council for the Exploration of the Sea

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Free, Conjugated, and Bound Polyamines during the Cell Cycle of Synchronized Cell Suspension Cultures of Nicotiana tabacum

Cited by 45 publications

References 19 publications

Immunocytochemical study of cell cycle control by cytokinin in cultured soybean cells

Immunocytochemical study of cell cycle control by cytokinin in cultured soybean cells

Polyamine Sparing May Be Involved in the Prolongation of Cell Division Due to Inhibition of Phenylpropanoid Synthesis in Cytokinin-Starved Soybean Cells

Detection of free polyamine in coastal seawater using ion exchange chromatography

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