Free and Conjugated Amines in Human Lumbar Cerebrospinal Fluid

Tyce, Gertrude M.; Rorie, Duane K.; Byer, David E.; Danielson, David R.

doi:10.1111/j.1471-4159.1985.tb07149.x

Cited by 35 publications

(15 citation statements)

References 11 publications

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“…26 showing a decrease in conjugated CSF-DA concentrations in successive samples of human lumbar CSF covering a total amount of 90 ml. Moreover slight support for our results is found in the studies by Sharpless et al 2~ Tyce et al 25 suggesting higher levels of conjungated DA at the lumbar than at the ventricular level. Moreover, the high correlation between L-CSF-DA and V-CSF-DA suggests that DA concentrations measured at the lumbar levels states the concentrations measured at the ventricular level.…”

Section: Discussionsupporting

confidence: 57%

Do concentrations of neurotransmitters measured in lumbar cerebrospinal fluid reflect the concentrations at brain level?

Gjerris

Sørensen

et al. 1988

Acta neurochir

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CSF concentrations of vasoactive intestinal polypeptide (VIP), cholecystokinin (CCK), noradrenaline (NA) and dopamine (DA) were measured in the lateral ventricles and at the lumbar level in patients with normal pressure hydrocephalus (NPH). The concentrations of VIP (n = 15), NA (n = 10) and DA (n = 10) were significantly higher at the lumbar level than at the ventricular level, whereas the concentrations of CCK (n = 9) were similar at the two sites. A significant positive correlation between the concentrations measured at the two levels was found for VIP (rs = 0.65; p less than or equal to 0.01) and DA (rs = 0.94; p less than or equal to 0.001). The results indicate that the concentrations of transmitter substances measured in CSF at the lumbar level not necessarily are indicative for concentrations measured more centrally. The negative correlations between Evans ratio and L-CSF VIP (rs = -0.76; p less than or equal to 0.001), and between resistance to outflow and V-CSF as well as L-CSF CCK (rs = -0.75); p less than or equal to 0.05) might be explained by a reduction in number of cortical neurons or by disturbances in CSF dynamics in patients with NPH.

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Section: Discussionsupporting

confidence: 57%

Do concentrations of neurotransmitters measured in lumbar cerebrospinal fluid reflect the concentrations at brain level?

Gjerris

Sørensen

et al. 1988

Acta neurochir

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“…Initially, a considerable amount of acid-hydrolysable conjugates of dopamine, norepinephrine (NE) and 5-HT were detected in lumbar CSF of normal individuals. The amounts of conjugated amines were small in comparison to the amounts of homovanillic acid and 5-hydroxyindoleaceticacid [9]. In a further study performed with CSF from humans and ventriculocisternal perfusion of African green monkeys found that the sulphates of NE, dopamine and 5-HT are present in the CSF of laboratory animals and humans.…”

Section: Literature Evidence Related To 5-ht-so 4 Appearance In Animamentioning

confidence: 92%

Application of 5-HT-SO4 in Biomarker Research

Lozda¹

2017

Serotonin - A Chemical Messenger Between All Types of Living Cells

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A serotonin catabolite, serotonin O-sulphate (5-HT-SO 4 ), is hypothesised to accentuate the intensity of serotonin metabolism in the central nervous system (CNS). We hypothesised that serotonin O-sulphate could be quantiied in human plasma using modern liquid chromatography-mass spectrometry. To test our hypothesis, we performed a critical literature review and a three-stage trial. First, a suitable liquid chromatography-mass spectrometry (LC-MS/MS) method for detection of 5-HT-SO 4 in human plasma samples was developed. Second, a pilot phase involving four healthy volunteers was executed. Finally, nine healthy volunteers were selected for the main study, where a basal plasma level of 5-HT-SO 4 was measured before and after serotonergic stimulation of the central nervous system. One h after stimulation, six study subjects showed a decrease in 5-HT-SO 4 levels, while three subjects showed an increase. This was the irst study in which naturally occurring 5-HT-SO 4 was detected by liquid chromatography-mass spectrometry (LC-MS/MS) in the samples of human plasma obtained from healthy volunteers. The method developed was speciic to the measurement of 5-HT-SO 4 and opens up new possibilities to evaluate minor pathways or serotonin metabolism by minimally invasive methods.

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“…Homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5HIAA) were measured after deproteinization with perchloric acid by high-performance liquid chromatography with electrochemical detection (Palfreyman et al, 1982;Silverstein et al, 1984;Tyce et al, 1985). Twenty microliters of the supernatant were injected directly into the system which included a precolumn (BST-GMK) and a 4 x 250 mm, 7 gm reverse phase column (Separon C-18).…”

Section: Methodsmentioning

confidence: 99%

Effect of adjuvant reserpine treatment on catecholamine metabolism in schizophrenic patients under long-term neuroleptic treatment

Bagdy

Perenyi

Frecska

et al. 1988

J. Neural Transmission

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The clinical and biochemical effects of adjuvant reserpine treatment were investigated in 12 chronic schizophrenic patients on long-term neuroleptic medication. The global severity of the symptoms using the Brief Psychiatric Rating Scale did not change significantly in the whole group, however, a moderate decrease in positive symptoms (factors though disturbance, activation and hostile-suspiciousness) was observed for 5 patients. Cerebrospinal fluid (CSF) noradrenaline levels showed a consistent decrease, but other biochemical parameters (CSF dopamine metabolites, platelet MAO and serum dopamine-beta-hydroxylase activities) did not change significantly. The changes of clinical symptoms and biochemical parameters did not show any correlation.

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Free and Conjugated Amines in Human Lumbar Cerebrospinal Fluid

Cited by 35 publications

References 11 publications

Do concentrations of neurotransmitters measured in lumbar cerebrospinal fluid reflect the concentrations at brain level?

Do concentrations of neurotransmitters measured in lumbar cerebrospinal fluid reflect the concentrations at brain level?

Application of 5-HT-SO4 in Biomarker Research

Effect of adjuvant reserpine treatment on catecholamine metabolism in schizophrenic patients under long-term neuroleptic treatment

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