Free Amino Acids in Semen: Measurement and Significance in Normal and Oligozoospermic Men

Silvestroni, L.; Morisi, G; Malandrino, F.; Frajese, Gaetano

doi:10.3109/01485017908987322

Cited by 8 publications

(5 citation statements)

References 8 publications

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“…Moreover, sperm concentration was increased significantly (P<0.05) with supplementing diet with 0.2 % Gly (T3) compared with control (T1), whereas, the data declare that all treatments did not show any significant change in sperm motility %, live sperm %, dead sperm %, abnormality sperm % and pH of M cocks among all experiment treatments. These results are in agreement with Silvestroni et al (1979) who found that semen plasma amino acids are lower in infertile patients compared to fertile persons, so, it was proposed that amino acids can protect sperms in the hostile vaginal environment. Glycine is the second highest concentration of free amino acids in bull semen plasma (Assumpção et al, 2005).…”

Section: Results and Discussion Semen Qualitysupporting

confidence: 92%

“…Ohta et al (1999) indicated that, amino acids administration in ovo at a late stage of incubation may boost the status of chicken embryo amino acid. Silvestroni et al, (1979) found that semen plasma amino acids are lower in infertile patients compared to fertile persons, so, it was proposed that amino acids can protect sperms in the hostile vaginal environment. Glycine is the second highest concentration of free amino acids in bull semen plasma (Assumpção et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Glycine Supplementation of Mandarah Local Chickens Dietary on Physiological and Reproductive Performance

Amin

Yassein

Attia

et al. 2022

EPSJ

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This study was conducted to evaluate the effect of dietary supplemental of glycine (Gly) on modulating physiological and reproduction performance of Mandarah (M) local chickens. A total of 135 hens and 18 cocks, from 28 to 40 weeks of age were randomly assigned to 3 treatments, and each treatment (T) include 45 hens and 6 cocks divided in 3 replicates of 15 hens and 2 cocks each. The 1 st group (T1) was fed the basal diet and served as a control group (without supplementation). The 2 nd group and 3 rd group were fed the basal diet supplemented with 0.1 and 0.2 %, Gly (1 and 2 gm/ kg diet), respectively.). The study showed that the cocks Gly treatments (0.1 and 0.2 %) were significantly (P<0.05) increased in the ejaculate volume, total sperm output, semen quality factor, total motile sperm, thyroxine (T4) and folliclestimulating hormones (FSH) compared with control treatment. Chickens that received diet supplemented with 0.1 % Gly had significant higher sperm concentration, total antioxidant capacity (TAC), triiodothyronine (T3) than those of control T1. No significant differences among T2, T3 and control (T1) in sperm motility %, live sperm %, dead sperm %, abnormal sperm %, fertility, hatchability, catalase, glutathione enzyme and luteinizing hormone (LH). Conclusively, it could be recommended to supply layer diets with Gly for better physiologically performance during the laying period.

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Section: Results and Discussion Semen Qualitysupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Effect of Glycine Supplementation of Mandarah Local Chickens Dietary on Physiological and Reproductive Performance

Amin

Yassein

Attia

et al. 2022

EPSJ

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“…This finding coincided with those of Hopwood and Gassner (1962) who reported that increased concentration of seminal total free amino acids was related to the increased quality and fertility of semen, and that poor semen quality was related to amino acids imbalance. Furthermore, a quantitative reduction in the concentration of all seminal plasma free amino acids has been observed in cases of azoospermia (Chaudhury et al, 2002;Papp et al, 1983) and oligospermia (Silvestroni et al, 1979).…”

Section: Amino Acidsmentioning

confidence: 98%

Molecular approach of gossypol-induced reproductive toxicity in male rabbits. Changes in seminal plasma amino acids and fatty acids

Taha

Shaaban

El-Nouty

et al. 2008

Animal

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This study was done to evaluate the effects of two sublethal doses of gossypol (4 and 20 mg/kg of BW, every other day) on some amino and fatty acid concentrations in male rabbit seminal plasma. Rabbits were chosen as an experimental animal owing to the fact that they are excellent model for reproductive toxicological effects. The experiment lasted 16 weeks and included two periods: a treatment period (first 8 weeks) where the animals were given the tested product, and a recovery period (second 8 weeks) where all drugs were withdrawn. Results showed that total amino acids (TAA), total essential amino acids (EAA), total non-essential amino acids (non-EAA) and EAA/non-EAA ratio were decreased in a dose-dependent manner during gossypol treatment. The deleterious effect on TAA concentrations was mainly due to the reduction in total EAA. However, these concentrations regained their normal values after gossypol cessation. Basic, acidic, neutral amino acids and basic/acidic amino acids ratio decreased in a dose-dependent manner by gossypol treatment. Additionally, gossypol administration caused decreases in total unsaturated fatty acids (USFA) and increases in total saturated fatty acids (SFA) and the SFA/USFA ratio in a dose-dependent manner. During the recovery period, total SFA and USFA showed significant reduction and significant increase, respectively, after gossypol withdrawal. In conclusion, gossypol administration affected rabbit seminal plasma concentrations of amino and fatty acids in a dose-dependant manner. Gossypol reduced TAA, total EAA and total non-EAA. Additionally, gossypol caused decreases in total USFA and increases in total SFA. These deleterious effects were associated with poor-quality semen observed in our previous studies.

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“…At present, very limited data on the metabolome of healthy human semen are available. For human seminal plasma, some data on metabolites are available in the literature [5,6]. The correlation analysis of the spermiogram parameters obtained by a computer-assisted sperm analysis (CASA) and sperm metabolites have also been reported [6].…”

Section: Introductionmentioning

confidence: 99%

Metabolomic profiling reveals correlations between spermiogram parameters and the metabolites present in human spermatozoa and seminal plasma

et al. 2019

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In 50% of all infertility cases, the male is subfertile or infertile, however, the underlying mechanisms are often unknown. Even when assisted reproductive procedures such as in vitro fertilization and intracytoplasmic sperm injection are performed, the causes of male factor infertility frequently remain elusive. Since the overall activity of cells is closely linked to their metabolic capacity, we analyzed a panel of 180 metabolites in human sperm and seminal plasma and elucidated their associations with spermiogram parameters. Therefore, metabolites from a group of 20 healthy donors were investigated using a targeted LC-MS/MS approach. The correlation analyses of the amino acids, biogenic amines, acylcarnitines, lysophosphatidylcholines, phosphatidylcholines, sphingomyelins and sugars from sperm and seminal plasma with standard spermiogram parameters revealed that metabolites in sperm are closely related to sperm motility, whereas those in seminal plasma are closely related to sperm concentration and morphology. This study provides essential insights into the metabolome of human sperm and seminal plasma and its associations with sperm functions. This metabolomics technique could be a promising screening tool to detect the factors of male infertility in cases where the cause of infertility is unclear.

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Free Amino Acids in Semen: Measurement and Significance in Normal and Oligozoospermic Men

Cited by 8 publications

References 8 publications

Effect of Glycine Supplementation of Mandarah Local Chickens Dietary on Physiological and Reproductive Performance

Effect of Glycine Supplementation of Mandarah Local Chickens Dietary on Physiological and Reproductive Performance

Molecular approach of gossypol-induced reproductive toxicity in male rabbits. Changes in seminal plasma amino acids and fatty acids

Metabolomic profiling reveals correlations between spermiogram parameters and the metabolites present in human spermatozoa and seminal plasma

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