Frecuencia y patotipos de Escherichia coli diarrogénicas en niños peruanos con y sin diarrea

Ochoa, Theresa J.; Mercado, Erik H.; Durand, David; Rivera, Fulton P.; Mosquito, Susan; Contreras, Carmen; Riveros, Maribel; Lluque, Angela; Barletta, Francesca; Prada, Ana; Ruiz, Joaquı́m

doi:10.1590/s1726-46342011000100003

Cited by 34 publications

(17 citation statements)

References 25 publications

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“…Although many studies have found a significant association of EPEC with infant diarrhea compared to control samples (14–16), several studies have found EPEC with similar frequency among diarrheal and control samples (11, 17–19). Healthy carriage of enteric pathogens in general is very common in developing countries.…”

Section: Epec Epidemiologymentioning

confidence: 95%

“…We have recently studied diarrheagenic E. coli prevalence in Peru using 8,000 E. coli strains previously isolated from 8 different studies in children mainly younger than 36 months of age and primarily from cohort studies in peri-urban Lima (11). Diarrheagenic E. coli were detected by a multiplex real-time PCR using specific primers for each of the six diarrheagenic E. coli (12, 13).…”

Section: Epec Epidemiologymentioning

confidence: 99%

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Enteropathogenic Escherichia coli infection in children

Ochoa

Contreras

2011

Current Opinion in Infectious Diseases

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244

193

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Purpose of review Enteropathogenic Escherichia coli (EPEC) are important diarrheal pathogens of young children. Since the diagnosis of EPEC is now based mainly on molecular criteria, there has been an important change in their prevalence. The purpose of this paper is to review the current epidemiology of EPEC infection and the new insights into its physiopathology. Recent findings Recent epidemiological studies indicate that atypical-EPEC (aEPEC) are more prevalent than typical-EPEC (tEPEC) in both developed and developing countries, and that aEPEC are important in both pediatric endemic diarrhea and diarrhea outbreaks. Therefore, it is important to further characterize the pathogenicity of these emerging strains. The virulence mechanisms and physiopathology of the attaching and effacing lesion (A/E) and the type-three-secretion-system (T3SS) are complex but well studied. A/E strains use their pool of locus of enterocyte effacement (LEE)-encoded and non-LEE-encoded effector proteins to subvert and modulate cellular and barrier properties of the host. However, the exact mechanisms of diarrhea in EPEC infection are not completely understood. Summary Remarkable progress has been made to identify virulence determinants required to mediate the pathogenesis of EPEC. However, fast, easy and inexpensive diagnostic methods are needed in order to define optimal treatment and prevention for children in endemic areas.

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Section: Epec Epidemiologymentioning

confidence: 95%

Section: Epec Epidemiologymentioning

confidence: 99%

Enteropathogenic Escherichia coli infection in children

Ochoa

Contreras

2011

Current Opinion in Infectious Diseases

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244

193

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“…In endemic areas, the colostrum of puerperal women is rich in sIgA against EPEC (48-50). A study in Peru showed that EPEC prevalence increased with age within the first two years of age (51). EPEC was found in 3% of diarrhea samples in children < 6 months old, in 11% of children 6-12 months old, and in 16% of children 13-24 months old.…”

Section: Asymptomatic Hosts Carrying Epecmentioning

confidence: 99%

Enteropathogenic Escherichia coli: foe or innocent bystander?

Torres

2015

Clinical Microbiology and Infection

147

142

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Enteropathogenic Escherichia coli (EPEC) remain one the most important pathogens infecting children and they are one of the main causes of persistent diarrhea worldwide. Historically, typical EPEC (tEPEC), defined as those isolates with the attaching and effacement (A/E) genotype (eae+), which possess bfpA+ and lack the stx- genes are found strongly associated with diarrheal cases. However, occurrence of atypical EPEC (aEPEC; eae+ bfpA- stx-) in diarrheal and asymptomatic hosts has made investigators question the role of these pathogens in human disease. Current epidemiological data is helping answering the question whether EPEC is mainly a foe or an innocent bystander during infection.

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“…El diagnostico de ECD es difícil, para identificarlas correctamente deben diferenciarse de miembros no patógenos de la biota normal, generalmente por determinación de los serotipos, lo cual no es determinante, pues muchas de estas bacterias pueden intercambiar material genético y con ello genes de virulencia, por lo que en los últimos años se ha elegido los métodos moleculares como la reacción en cadena de la polimerasa (PCR) para la detección con resultados rápidos, fiables, con alta sensibilidad y especificidad (7,8) basada en la identificación de la presencia de genes de virulencia específicos, que están ausentes en cepas no patógenas (9,10).…”

Section: Introductionunclassified

Molecular detection of diarrheogenic Escherichia coli in pediatric patients with acute diarrheal syndrome in Paraguay

Weiler

Orrego

Álvarez

et al. 2017

Mem. Inst. Investig. Cienc. Salud

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R E S U M E NLa Escherichia coli diarreogénica (ECD) se ha clasificado con base en criterios clínicos, epidemiológicos y moleculares en cinco grupos, cada uno con factores de virulencia específicos. El objetivo fue determinar la prevalencia de ECD en pacientes pediátricos con enfermedad diarreica aguda del Laboratorio Central de Salud Publica en el periodo 2012-2015. Se procesaron muestras de heces con síndrome diarreico agudo, provenientes de pacientes pediátricos, en los cuales se buscó algún gen de virulencia ECD utilizando métodos convencionales de siembra y screening molecular, mediante PCR múltiple con cebadores diseñados específicamente para amplificar los genes de virulencia elt, est, eae, stx, ipaH y aggR. Del total de muestras analizadas, 13% (180/1379) de las muestras presentó algún factor de virulencia compatible con algún patotipo ECD con mayor predominio en niños de 1 a 3 años. La frecuencia de los distintos patotipos fue la siguiente: 61 (34%) ETEC, 40 (22%) EAEC, 41 (23%) EPEC, 27 (15%) EIEC, 7 (4%) STEC y 3 (2%) ETEC/EAEC, 1 (0.5%) ETEC/EAEC/EIEC. El porcentaje de E. coli diarreogénicas detectado tiene similitud con lo reportado en otros países de la región, lo que nos indica que estos patógenos son parte importante de la etiología de la enfermedad diarreica aguda infecciosa en la población infantil en nuestro país. Se debe destacar que para el diagnóstico de las diferentes categorías ECD, es necesario disponer de un procedimiento diagnóstico específico dirigido a la detección de los factores de virulencia utilizando métodos moleculares o métodos inmunológicos.Palabras clave: Escherichia coli diarreogénicas, Enfermedad Diarreica Aguda, PCR.

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Frecuencia y patotipos de Escherichia coli diarrogénicas en niños peruanos con y sin diarrea

Abstract: DEC are a major cause of diarrhea in Peruvian children. These pathogens are highly heterogeneous. Additional studies are required to determine the prevalence in rural areas of Peru and in severe diarrhea cases.

Cited by 34 publications

References 25 publications

Enteropathogenic Escherichia coli infection in children

Enteropathogenic Escherichia coli infection in children

Enteropathogenic Escherichia coli: foe or innocent bystander?

Molecular detection of diarrheogenic Escherichia coli in pediatric patients with acute diarrheal syndrome in Paraguay

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