Frameshift mutations induced by three classes of acridines in the <i>lacZ</i> reversion assay in <i>Escherichia coli</i>: Potency of responses and relationship to slipped mispairing models

Hoffmann, George; Calciano, Margaret A.; Lawless, Bryan M.; Mahoney, Kathleen M.

doi:10.1002/em.10182

Cited by 37 publications

(40 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Drugs inhibit DNA synthesis by two mechanisms that are generally associated: the drug either binds to DNA by intercalation and stops the replication ; or interferes directly with molecules required for DNA polymerization and/or initiation of replication (Pommier et al, 1998;Bruning & Mylonas et al, 2011). The ability of such drugs to intercalate into DNA can induce mutations in normal cells (Hoffmann et al, 2003). The accumulation of several random mutations can lead to aberrations in the cells and conversion of non-carcinogenic cells into carcinogenic cells (Ferguson & Denny, 1995;Raynaud et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

Assessment of estrogenic, mutagenic and antimutagenic activity of nemorosone

Camargo¹,

Varela²,

Oliveira³

et al. 2011

Rev. bras. farmacogn.

View full text Add to dashboard Cite

Currently, a wide range of research involving natural products is focused on the discovery of new drugs in many different therapeutic areas. A great number of the synthetic compounds on the market were derived from natural products, especially plants. Nemorosone is the major constituent of the floral resin of Clusia rosea Jacq., Clusiaceae, and in Cuban propolis. In vitro studies have shown cytotoxic activity in this substance against various tumor cell lines, including those resistant to various cytotoxic drugs, whereas it has low cytotoxicity to non-tumoral cells. Therefore, in order to characterize the biological activity of nemorosone, a substance with potential antitumor activity, and in view of preclinical testing of the toxicity of drug candidate compounds, the main aim of this study was to determine the mutagenic and antimutagenic activity of nemorosone by the Ames test, using the strains TA97a, TA98, TA100 and TA102 of Salmonella typhimurium. Secondly, to characterize the estrogenic activity in an experimental recombinant yeast model (Recombinant Yeast Assay) mutagenic activity was observed at in any of the concentrations in any of the test strains. To evaluate the antimutagenic potential, direct and indirect mutagenic agents were used: 4 nitro-o-phenylenediamine (NPD), mitomycin C (MMC) and aflatoxin B1 (AFL). Nemorosone showed moderate antimutagenic activity (inhibition level 31%), in strain TA100 in the presence of AFL, and strong antimutagenic activity in TA102 against MMC (inhibition level 53%). Estrogenic activity was observed, with an EEq of 0.41±0.16 nM at various tested concentrations.

show abstract

Section: Resultsmentioning

confidence: 99%

Assessment of estrogenic, mutagenic and antimutagenic activity of nemorosone

Camargo¹,

Varela²,

Oliveira³

et al. 2011

Rev. bras. farmacogn.

View full text Add to dashboard Cite

show abstract

“…Through this way, 9-AA induces frameshift mutations at hot spots where a single base, especially guanine, is repeated (Hoffmann et al, 2003).…”

Section: Genotoxic and Antigenotoxic Activity Evaluationmentioning

confidence: 99%

Two antigenotoxic chalcone glycosides fromMentha longifoliasubsp.longifolia

Güvenalp

Özbek

Karadayı

et al. 2014

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Mentha L. (Labiatae) species (mint) with their flavoring properties have been used in food industries for centuries. Besides they have a great importance in drug development and medicinal applications due to various bioactive compounds of several members of the genus. Objective: The aim of this study was to isolate bioactive compounds with antimutagenic potential by bio-guided fractionation and determine their structures by spectroscopic methods. Materials and methods: The structural elucidation of the isolated compounds was done based on spectroscopic methods, including MALDI-MS, UV, IR, and 2D NMR experiments, and the bioguided fractionation process was done by using the Ames/Salmonella test system. Henceforth, solely genotoxic and antigenotoxic potential of the new compounds were also confirmed up to 2 mM/plate by using the same test system. According to the antimutagenicity results, both new test compounds significantly inhibited the mutagenic activity of 9-aminoacridine in a dose-dependent manner at the tested concentrations from 0.8 to 2 mM/plate. (bR)-b,4,2 0 ,6 0 -Tetrahydroxy-4 0 -O-rutinosyldihydrochalcone showed the maximum inhibition rate as 75.94% at 2 mM/plate concentration. Conclusions: This is the first report that two new chalcone glycosides were isolated from Mentha longifolia subsp. longifolia and their antimutagenic potentials by using mutant bacterial tester strains. In conclusion, the two new chalcone glycosides showed a significant antigenotoxic effect on 9-aminoacridine-induced mutagenesis at tested concentrations.

show abstract

“…9-AA used in this study is a simple intercalator. Through intercalation, 9-AA induces frame-shift mutations at hotspots in which a single base, especially guanine, is repeated [40,[46][47][48]. The antimutagenicity assay performed with S. typhimurium TA1537 and 9-aminoacridine depends on the inhibition of this mechanism by test substances, which were thought as antimutagenic.…”

mentioning

confidence: 99%