Fragmentation patterns study of iridoid glycosides in Fructus Gardeniae by HPLC‐Q/TOF‐MS/MS

Fu, Zhiwen; Xue, Rui; Li, Zhixiong; Chen, Mingcang; Sun, Zhaolin; Hu, Yiyang; Huang, Chenggang

doi:10.1002/bmc.3223

Cited by 28 publications

(31 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Relying on the comparable fragmentation pattern given by Fu et al. , compound 23 was proposed as geniposide.…”

Section: Resultsmentioning

confidence: 79%

Determination of phenolic compounds and antioxidant activities from peel, flesh, seed of guava (Psidium guajava L.)

et al. 2018

Electrophoresis

View full text Add to dashboard Cite

White-flesh guava is widely planted in tropical or subtropical areas of Southeast Asia. Despite of folk statements on specific function, few researches are focused on the description of its plant secondary metabolites. In the present work, contents of total phenolics and flavonoids as well as antioxidant activity from different parts (peel, flesh, and seed) were determined. The constituents of ethanol extracts were characterized by HPLC-QTOF-MS. A total of 69 phenolic compounds as well as nine polar compounds were detected, with flavonoids, hydrolyzable tannins, phenolic acid derivatives, and benzophenones of the four predominant phenolic compounds. Moreover, the presence of other phenolics (lignan, phenylethanoid, stilbenoid, and dihydrochalcones) was revealed. Simultaneously, the polar compounds, such as triterpenoids, iridoid were identified. Benzophenones and triterpenoids were proved to be marked constitutes of peel and flesh, respectively. The existence of isoflavonoids, lignan, phenylethanoid were firstly reported for edible parts or by-products of guava. The results showed that white-flesh guavas, particularly peel parts, were superior resources of antioxidant compounds, with exploitation value.

show abstract

“…Relying on the comparable fragmentation pattern given by Fu et al. , compound 23 was proposed as geniposide.…”

Section: Resultsmentioning

confidence: 79%

Determination of phenolic compounds and antioxidant activities from peel, flesh, seed of guava (Psidium guajava L.)

et al. 2018

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…In comparison with the conventional HPLC method, ultra‐performance liquid chromatography (UPLC) has the advantages of shorter retention time, higher analytical sensitivity and excellent peak capacity (Liu et al ., ; Yan et al ., ). In addition, a quadrupole time‐of‐flight (Q/TOF) mass analyzer operating in MS E mode allows the selection and isolation of precursor ions with high efficiency, sensitivity and mass accuracy (Fu et al ., ; Zhao et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Rapid screening and identification of lycodine‐type alkaloids in Lycopodiaceae and Huperziaceae plants by ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry

Shan

Luo

Pan

et al. 2016

Biomedical Chromatography

View full text Add to dashboard Cite

Lycodine-type alkaloids have gained significant interest owing to their unique skeletal characteristics and acetylcholinesterase activity. This study established a rapid and reliable method using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q/TOF-MS/MS) for comprehensive characterization of lycodine-type alkaloids for the first time. The lycodine-type alkaloids were detected successfully from Lycopodiastrum casuarinoides, Huperzia serrata and Phlegmarirus carinatus in seven plants of the Lycopodiaceae and Huperziaceae families, based on the established characteristic MS fragmentation of five known alkaloids. Furthermore, a total of 13 lycodine-type alkaloids were identified, of which three pairs of isomers were structurally characterized and differentiated. This study further improves mass analysis of lycodine-type alkaloids and demonstrates the superiority of UPLC with a high-resolution mass spectrometer for the rapid and sensitive structural elucidation of other trace active compounds. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

“…In the present study, HPLC‐DAD‐ESI‐Q‐TOF MS/MS was used to provide characteristic fragmentation patterns that allowed rapid structural identification of potential HOCl scavengers from GJE. Twenty‐six compounds from GJE (Table ) were characterized by MS data and UV spectra, using comparisons with published data .…”

Section: Resultsmentioning

confidence: 99%

A novel strategy for evaluation of natural products acting on the myeloperoxidase/hypochlorous acid system by combining high‐performance liquid chromatography‐photodiode array detection‐chemiluminescence and ultrafiltration‐mass spectrometry techniques

Wang

Fang

et al. 2018

J of Separation Science

View full text Add to dashboard Cite

Myeloperoxidase and its product hypochlorous acid are endogenous substances that are involved in the pathogenesis of many inflammatory diseases. In the present study, a novel evaluation strategy has been developed to screen for myeloperoxidase inhibitors and hypochlorous acid scavengers in natural products. The evaluation strategy uses two methods: a multi-hyphenated high-performance liquid chromatography-photodiode array detection-chemiluminescence online method, and an ultrafiltration-high-performance liquid chromatography-mass spectrometry method. Both were used for screening hypochlorous acid scavengers and myeloperoxidase inhibitors. After optimization of operating conditions, including pH of mobile phase and concentrations of myeloperoxidase, hypochlorous acid and luminol, the evaluation strategy was applied to Gardenia jasminoides Ellis (Rubiaceae) extract. Gardenia jasminoides Ellis (Rubiaceae) extract was found to both scavenge hypochlorous acid and inhibit myeloperoxidase. These different bioactivities originate from iridoid glycosides and quinic acid derivatives (25 hypochlorous acid scavengers) and crocetin derivatives (4 myeloperoxidase inhibitors). In a mouse model of acute lung injury, pulmonary histopathology showed that different constituents of Gardenia jasminoides Ellis (Rubiaceae) extract could also attenuate lung injury. Although the screening strategy has two arms, it is still simple, rapid, and effective. The strategy can also potentially be used to discriminate different activities of different constituents contained in the same natural product.

show abstract

Fragmentation patterns study of iridoid glycosides in Fructus Gardeniae by HPLC‐Q/TOF‐MS/MS

Cited by 28 publications

References 21 publications

Determination of phenolic compounds and antioxidant activities from peel, flesh, seed of guava (Psidium guajava L.)

Determination of phenolic compounds and antioxidant activities from peel, flesh, seed of guava (Psidium guajava L.)

Rapid screening and identification of lycodine‐type alkaloids in Lycopodiaceae and Huperziaceae plants by ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry

A novel strategy for evaluation of natural products acting on the myeloperoxidase/hypochlorous acid system by combining high‐performance liquid chromatography‐photodiode array detection‐chemiluminescence and ultrafiltration‐mass spectrometry techniques

Contact Info

Product

Resources

About