Fragmentation patterns of five types of phospholipids by ultra-high-performance liquid chromatography electrospray ionization quadrupole time-of-flight tandem mass spectrometry

Pi, Juanjuan; Wu, Xia; Feng, Yifan

doi:10.1039/c5ay00776c

Cited by 54 publications

(60 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These characteristic ions are summarized in Table 2 , which are consistent with the fragmentation patterns of phospholipids previously reported. 27 , 28 Representative fragmentation schemes of these synthetic [ 13 C 6 ]Amadori-phospholipids are shown in Figure 8 , taking [ 13 C 6 ]Amadori-PE(16:0/18:1(9Z)) and [ 13 C 6 ]Amadori-PS(16:0/18:1(9Z)) as examples.…”

Section: Resultsmentioning

confidence: 99%

Synthesis, Purification, and Mass Spectrometric Characterization of Stable Isotope-Labeled Amadori-Glycated Phospholipids

Zhang

2018

ACS Omega

View full text Add to dashboard Cite

Nonenzymatic glycation of lipids plays an important role in several physiological and pathological processes, such as normal aging and complications of diabetes mellitus. To develop liquid chromatography coupled with mass spectrometric (LC-MS) methods for accurate analysis of Amadori compound-glycated lipids from biological samples, it is essential to obtain isotope-labeled Amadori-lipid standards. Herein, we report optimized methods for the preparation of six stable isotope-labeled Amadori-glycated lipid standards covering four types of lipids, including [13C6]Amadori-phosphatidyl ethanolamine (PE), -phosphatidyl serine (PS), -LysoPE, and -LysoPS. Optimal conditions for the synthesis and purification of these four types of Amadori-glycated lipids were detailed in this study. LC-MS and LC-UV analyses showed that destination products were highly purified (>95%). Accurate mass and MS/MS fragmentation in both positive- and negative-ion modes further validated the identification of these six synthetic [13C6]Amadori-glycated lipid standards. Successful preparation of these highly purified isotope-labeled standards makes it possible to develop targeted LC-MS/MS methods for accurate analysis of Amadori-glycated phospholipids from biological samples.

show abstract

Section: Resultsmentioning

confidence: 99%

Synthesis, Purification, and Mass Spectrometric Characterization of Stable Isotope-Labeled Amadori-Glycated Phospholipids

Zhang

2018

ACS Omega

View full text Add to dashboard Cite

show abstract

“…The ratio of [FA 1 -H] − /[FA 2 -H] − has been used for sn-positional assignments; however, relative intensities of the two carboxylate anions can vary depending on length and unsaturation, as well as the collision energy used in fragmentation [27,31,41,42]. In this respect, it has been reported that different fragmentation patterns originated from different cleavage sites could be formed as a result of very different collision energies [43], or the ratio of [FA 1 -H] − /[FA 2 -H] − could be altered with the collision energy [29,31]. Hence, to confidently identify the sn-position assignment of fatty acyls, we studied the relative ratio of Results from mixed-mode LC separation showed that there were no noticeable lipid classlevel changes between healthy and T1D groups or between the early and late time points of each group.…”

Section: Discussionmentioning

confidence: 99%

“…However, because this species was from the PC fraction, we limited the database search space of LipidSearch to PC when processing the RPLC-MS/MS raw data of this fraction. Although fragment ion ratio of [FA 1 -H] − /[FA 2 -H] − < 1 has been commonly used to assign the sn-positions, there are reported exceptions to this rule and it casts doubt on solely relying on this ratio to assign the sn--position when using different collision energies [28][29][30][31].…”

Section: Rplc-ms/ms Analysis Of Lipid Class Fractions Improved Confidmentioning

confidence: 99%

Accurate mass and retention time library of serum lipids for type 1 diabetes research

Narváez-Rivas

Chen

et al. 2019

Anal Bioanal Chem

View full text Add to dashboard Cite

Dysregulated lipid species are linked to various disease pathologies and implicated as potential biomarkers for type 1 diabetes (T1D). However, it is challenging to comprehensively profile the blood specimen lipidome with full structural details of every lipid molecule. The commonly used reversed-phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS)-based lipidomics approach is powerful for the separation of individual lipid species, but lipids belonging to different classes may still co-elute and result in ion suppression and misidentification of lipids. Using offline mixed-mode and RPLC-based two-dimensional separations coupled with MS/MS, a comprehensive lipidomic profiling was performed on human sera pooled from healthy and T1D subjects. The elution order of lipid molecular species on RPLC showed good correlations to the total number of carbons in fatty acyl chains and total number of double bonds. This observation together with fatty acyl methyl ester analysis was used to enhance the confidence of identified lipid species. The final T1D serum lipid library database contains 753 lipid molecular species with accurate mass and RPLC retention time uniquely annotated for each of the species. This comprehensive human serum lipid library can serve as a database for high-throughput RPLC-MSbased lipidomic analysis of blood samples related to T1D and other childhood diseases.

show abstract

“…Choline-containing phospholipid species, Phosphatidylcholine (PCs) and Sphingomyelin (SMs), both show a characteristic m / z 184 phosphocholine head group peak, as well as an [M + H-59] + peak corresponding to the neutral loss of (CH 3 ) 3 N. Whereas fragmentation of phosphatidylethanolamines exclusively yields one peak, an [M + H-141] + ion from the neutral loss of the phosphoethanolamine head group and fragmentation of phosphatidylserines yields an [M + H-185] + ion from the neutral loss of the phosphatidylserine head group (Pi et al 2016). …”

Section: Methodsmentioning

confidence: 99%

Ultra high performance liquid chromatography–high resolution mass spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets

et al. 2017

View full text Add to dashboard Cite

Introduction and objectivesThe purpose of this study was to use high accurate mass metabolomic profiling to investigate differences within a phenotypically diverse canine population, with breed-related morphological, physiological and behavioural differences. Previously, using a broad metabolite fingerprinting approach, lipids appear to dominate inter- and intra- breed discrimination. The purpose here was to use Ultra High Performance Liquid Chromatography–High Resolution Mass Spectrometry (UHPLC–HRMS) to identify in more detail, inter-breed signatures in plasma lipidomic profiles of home-based, client-owned dogs maintained on different diets and fed according to their owners’ feeding regimens.MethodsNine dog breeds were recruited in this study (Beagle, Chihuahua, Cocker Spaniel, Dachshund, Golden Retriever, Greyhound, German Shepherd, Labrador Retriever and Maltese: 7–12 dogs per breed). Metabolite profiling on a MTBE lipid extract of fasted plasma was performed using UHPLC-HRMS.ResultsMultivariate modelling and classification indicated that the main source of lipidome variance was between the three breeds Chihuahua, Dachshund and Greyhound and the other six breeds, however some intra-breed variance was evident in Labrador Retrievers. Metabolites associated with dietary intake impacted on breed-associated variance and following filtering of these signals out of the data-set unique inter-breed lipidome differences for Chihuahua, Golden Retriever and Greyhound were identified.ConclusionBy using a phenotypically diverse home-based canine population, we were able to show that high accurate mass lipidomics can enable identification of metabolites in the first pass plasma profile, capturing distinct metabolomic variability associated with genetic differences, despite environmental and dietary variability.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-016-1152-0) contains supplementary material, which is available to authorized users.

show abstract

Fragmentation patterns of five types of phospholipids by ultra-high-performance liquid chromatography electrospray ionization quadrupole time-of-flight tandem mass spectrometry

Abstract: The fragmentation regularities of five phospholipids have been studied by ultra-high-performance liquid chromatography electrospray ionization quadrupole time-of-flight tandem mass spectrometry.

Cited by 54 publications

References 37 publications

Synthesis, Purification, and Mass Spectrometric Characterization of Stable Isotope-Labeled Amadori-Glycated Phospholipids

Synthesis, Purification, and Mass Spectrometric Characterization of Stable Isotope-Labeled Amadori-Glycated Phospholipids

Accurate mass and retention time library of serum lipids for type 1 diabetes research

Ultra high performance liquid chromatography–high resolution mass spectrometry plasma lipidomics can distinguish between canine breeds despite uncontrolled environmental variability and non-standardized diets

Contact Info

Product

Resources

About