“…In isolation, each of the techniques FP, FRET, ITC, and SPR act as a unique lens through which to study 14-3-3 PPIs, but these methods alone do not provide a full picture. Other approaches not discussed here (e.g., microscale thermophoresis (Centorrino et al, 2022;Kozeleková et al, 2022), differential scanning fluorimetry (Cossar et al, 2021;Waløen et al, 2021;Joshi et al, 2022), analytical ultracentrifugation (Horvath et al, 2021;Leysen et al, 2021;Neves et al, 2021;Pohl et al, 2021;Srdanović et al, 2022), single angle x-ray scattering (Kast and Dominguez, 2019;Leysen et al, 2021) also contribute new understanding and support hypotheses. NMR (Kuusk et al, 2019;Neves et al, 2021) and native mass spectrometry (Bellamy-Carter et al, 2021) are also important techniques that shows great promise for studying 14-3-3 PPIs.…”