Fractionation of synaptic plasma membrane glycoproteins by lectin affinity chromatography

Gurd, James W.; Mahler, Henry R.

doi:10.1021/bi00722a022

Cited by 73 publications

(28 citation statements)

References 40 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The diffuse labeling of axoplasmic material, presynaptic boutons, and ribosomes support earlier findings by Castejon and Castejon [1972, 19761 which indicated that proteoglycans and possibly glycosaminoglycans were associated with these structures in a manner very similar to that presented here. The binding of wheat germ agglutinin to proteins of synaptic complexes has also been characterized by biochemical methods [Gurd, 1977a[Gurd, , 1977bGurd and Mahler, 1974;Zanetta et al, 19811. It is not clear which of these are involved in the histochemical preparations shown here.…”

Section: Disc Usslo Nmentioning

confidence: 99%

Sonication‐enhanced histochemistry of wheat germ agglutinin binding to intracellular sites of rat layer V cortical neurons and spinal motoneurons

Wells¹,

Bernstein²

1982

J of Neuroscience Research

View full text Add to dashboard Cite

Cellular surface carbohydrates have been studied extensively in relation to cell interactions. However, the presence of intracellular carbohydrates not associated with cell surface membranes has not been well-defined histochemically. We describe a technique for the ultrastructural localization of intracytoplasmic carbohydrates using a wheat germ agglutinin peroxidase conjugate. The ultrastructural aspects of wheat germ agglutinin (WGA) binding to intracellular structures of rat somatomotor cortex layer V neurons and spinal cord motoneurons were examined using sonication to enhance penetration of the lectin-peroxidase conjugate. In addition to the membrane-associated structures previously described by others, WGA binding was observed on neuronal nuclear chromatin, nuclear pores, ribosomes, microtubules, vesicular organelles, and to synaptic complexes, primarily in the presynaptic bouton. The results support data indicating that complex carbohydrates may be important in neuronal molecular processing.

show abstract

Section: Disc Usslo Nmentioning

confidence: 99%

Sonication‐enhanced histochemistry of wheat germ agglutinin binding to intracellular sites of rat layer V cortical neurons and spinal motoneurons

Wells¹,

Bernstein²

1982

J of Neuroscience Research

View full text Add to dashboard Cite

show abstract

“…Crude microsomal fraction P3 was further fractionated into four subfractions on discontinuous sucrose gradients as described by Curd et al (1974). These authors refer to the least dense fraction (Fraction 1) as smooth microsomes (SMic) and the most dense fraction (Fraction 4) as rough microsomes (RMic), based on the distribution of RNA and NADPH:cytochrome c reductase in similar fractions isolated from liver (Glauman and Dallner, 1970).…”

Section: Subcellular Fractionsmentioning

confidence: 99%

Distribution of Six Synaptic Membrane Antigens in Subcellular Fractions of Rat Brain Cortex

Mahadik

Korenovsky

Ciccarone

et al. 1981

Journal of Neurochemistry

View full text Add to dashboard Cite

The subcellular distribution in rat brain cortex of six synaptic membrane antigens (56K, 58K, 62K, 63K, 64K, 66K) was studied by rocket immunoelectrophoresis, using antiserum to a highly purified synaptic plasma membrane fraction. Initial analysis of the insoluble portion of subcellular fractions showed that these antigens were also present in smooth microsomes, rough microsomes, and synaptic vesicles; that only traces were present in synaptic junctions; and that none was present in nuclei, mitochondria, and myelin. A trace amount of activity was also present in synaptic vesicle cytosol, but none in whole brain cytosol. Quantitative measurements of synaptic plasma membranes, smooth microsomes, and synaptic vesicles showed that all six antigens were present in synaptic plasma membranes and smooth microsomes, but that the 66K antigen was absent from synaptic vesicles. The 56K, 58K, 62K, 63K, and 64K antigens were present in highest concentration in synaptic plasma membranes, whereas the 66K antigen content was highest in smooth microsomes. Only the 58K, 62K, and 63K antigens were detectable in the membrane fraction of whole brain. Their enrichments in synaptic plasma membranes were 10.9, 5.4, and 5.9, respectively. We conclude that the 56K, 58K, 62K, 63K and 64K antigens are primary components of synaptic plasma membranes. The presence of synaptic plasma membrane antigens in smooth microsomes and synaptic vesicles probably represents material being actively transported, consistent with the hypothesis that proteins of synaptic plasma membranes and synaptic vesicles are transported via smooth endoplasmic reticulum.

show abstract

“…Natori et ai'. (1977) (Gurd & Mahler, 1974 Immunogenicities were compared by calculating the antigenic ratio (Basombrio, 1970) …”

mentioning

confidence: 99%

Partial purification of tumour-specific transplantation antigens from methylcholanthrene-induced murine sarcomas by immobilized lectins

Sikora¹,

Koch²,

Brenner³

et al. 1979

Br J Cancer

View full text Add to dashboard Cite

Summary.-Plasma membranes isolated from two immunogenic, non-crossprotecting, MC sarcomas were shown to retain the specific rejection antigens of whole cells as well as serologically detected H-2 antigens. Solubilization of the membranes with sodium deoxycholate gave quantitative release of H-2 and retained the rejection specificity of the tumour from which it was derived. Polyacrylamide-gel electrophoresis (PAGE) (Basombrio, 1970

show abstract

Fractionation of synaptic plasma membrane glycoproteins by lectin affinity chromatography

Cited by 73 publications

References 40 publications

Sonication‐enhanced histochemistry of wheat germ agglutinin binding to intracellular sites of rat layer V cortical neurons and spinal motoneurons

Sonication‐enhanced histochemistry of wheat germ agglutinin binding to intracellular sites of rat layer V cortical neurons and spinal motoneurons

Distribution of Six Synaptic Membrane Antigens in Subcellular Fractions of Rat Brain Cortex

Partial purification of tumour-specific transplantation antigens from methylcholanthrene-induced murine sarcomas by immobilized lectins

Contact Info

Product

Resources

About