Fractionation of stable carbon isotopes of tissue fatty acids in Atlantic pollock (<i>Pollachius virens</i>)

Budge, Suzanne M.; AuCoin, Lacey R; Ziegler, Susan E.; Lall, Santosh P.

doi:10.1002/ecs2.1437

Cited by 17 publications

(36 citation statements)

References 59 publications

(51 reference statements)

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“…Diet tracing methods can be also combined non-independently, where diet information from one method is being integrated with another dietary technique . Recent advances in 2011), Budge et al (2016), Gladyshev et al (2012), Gladyshev et al (2016 and isotope data (Stock & Semmens, 2015) and with other biological data (Swanson et al, 2015). For instance, using dietary estimates of visual or molecular analyses as prior information for Bayesian isotope mixing models can improve quantitative diet estimates (Chiaradia et al, 2014).…”

Section: Integration Of Multiple Dietary Methodsmentioning

confidence: 99%

Diet tracing in ecology: Method comparison and selection

et al. 2017

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Determining diet is a key prerequisite for understanding species interactions, food web structure and ecological dynamics. In recent years, there has been considerable development in both the methodology and application of novel and more traditional dietary tracing methods, yet there is no comprehensive synthesis that systematically and quantitatively compares the different approaches. Here we conceptualise diet tracing in ecology, provide recommendations for method selection, and illustrate the advantages of method integration. We summarise empirical evidence on how different methods quantify diet mixtures, by contrasting estimates of dietary proportions from multiple methods applied to the same consumer‐resource datasets, or from experimental studies with known diet compositions. Our data synthesis revealed an urgent need for more experiential comparisons among the dietary methods. The comparison of diet quantifications from field observations showed that different techniques aligned well in cases with less than six diet items, but diverged considerably when applied to more complex diet mixtures. Efforts are ongoing to further advance dietary estimation, including how reliably compound specific stable isotope analyses and fatty acid profiles can quantify more prey items than bulk stable isotope analyses. Similarly, DNA analyses, which can depict trophic interactions at a higher resolution than any other method, are generating new ways to better quantify diets and differentiate among life‐stages of prey. Such efforts, combined with more empirical testing of each dietary method and establishment of open data repositories for dietary data, promise to greatly advance community and ecosystem ecology.

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Section: Integration Of Multiple Dietary Methodsmentioning

confidence: 99%

Diet tracing in ecology: Method comparison and selection

et al. 2017

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“…All rights reserved. deposition in the predator, their relative amounts may be altered owing to catabolism or selective deposition and mobilization [10][11][12]. Thus, a key aspect of this approach is the adjustment of the predator's FA profile for these modifications.…”

Section: Introductionmentioning

confidence: 99%

“…In QFASA applications, these modifications are taken into account using 'calibration coefficients' (CC) [13], defined as the ratio of the proportion of a FA in the predator's tissue relative to the proportion of the same FA in diet, after feeding the diet for a prolonged period [9]. While the basic biochemical processes that may occur are well known [12], the precise changes in FA concentration that arise from metabolism remain uncertain, making empirically derived CC essential.…”

Section: Introductionmentioning

confidence: 99%

Dietary fat concentrations influence fatty acid assimilation patterns in Atlantic pollock (Pollachius virens)

Budge

Townsend

Lall

et al. 2020

Phil. Trans. R. Soc. B

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A key aspect in the use of fatty acids (FA) to estimate predator diets using quantitative FA signature analysis (QFASA) is the ability to account for FA assimilation through the use of calibration coefficients (CC). Here, we tested the assumption that CC are independent of dietary fat concentrations by feeding Atlantic pollock ( Pollachius virens ) three formulated diets with very similar FA proportions but different fat concentrations (5–9% of diet) for 20 weeks. CC calculated using FA profiles of diet and triacylglycerols in pollock liver were significantly different for the three diets. To test the robustness of diet estimates to these differences, we used the CC set derived from feeding the diet with the lowest fat concentration, published prey FA profiles and realistic diet estimates of pollock to construct ‘pseudo-predators'. Application of QFASA to each pseudo-predator using the three sets of CC and the same prey FA profiles resulted in diet estimate biases of twofold for major prey items and approximately fivefold for minor prey items. This work illustrates the importance of incorporating diets with fat concentrations that are similar to natural prey when conducting feeding experiments to calculate CC. This article is part of the theme ‘The next horizons for lipids as ‘trophic biomarkers': evidence and significance of consumer modification of dietary fatty acids'.

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“…For tracing the dietary essential fatty acid origin, although their carbon stable isotope ratios would be helpful, information about isotopic fractionation of essential fatty acids between diet and consumers is limited. For instance, Budge et al [26] demonstrated that there was no isotopic fractionation in several essential fatty acids between the diet and serum of fish in a feeding experiment with Atlantic Pollock. Moreover, an almost isotopically unchanged transfer of 18:2ω6 and 18:3ω3 between diet and zebrafish Danio rerio was observed in a 100-day feeding experiment with constant dietary sources [27].…”

Section: Introductionmentioning

confidence: 99%

Origin of Carbon and Essential Fatty Acids in Higher Trophic Level Fish in Headwater Stream Food Webs

et al. 2019

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Dietary carbon sources in headwater stream food webs are divided into allochthonous and autochthonous organic matters. We hypothesized that: 1) the dietary allochthonous contribution for fish in headwater stream food webs positively relate with canopy cover; and 2) essential fatty acids originate from autochthonous organic matter regardless of canopy covers, because essential fatty acids, such as 20:5ω3 and 22:6ω3, are normally absent in allochthonous organic matters. We investigated predatory fish Salvelinus leucomaenis stomach contents in four headwater stream systems, which are located in subarctic region in northern Japan. In addition, stable carbon and nitrogen isotope ratios, fatty acid profile, and stable carbon isotope ratios of essential fatty acids were analyzed. Bulk stable carbon analysis showed the major contribution of autochthonous sources to assimilated carbon in S. leucomaenis. Surface baits in the stomach had intermediate stable carbon isotope ratios between autochthonous and allochthonous organic matter, indicating aquatic carbon was partly assimilated by surface baits. Stable carbon isotope ratios of essential fatty acids showed a positive relationship between autochthonous sources and S. leucomaenis across four study sites. This study demonstrated that the main supplier of dietary carbon and essential fatty acids was autochthonous organic matter even in headwater stream ecosystems under high canopy cover.

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Fractionation of stable carbon isotopes of tissue fatty acids in Atlantic pollock (Pollachius virens)

Cited by 17 publications

References 59 publications

Diet tracing in ecology: Method comparison and selection

Diet tracing in ecology: Method comparison and selection

Dietary fat concentrations influence fatty acid assimilation patterns in Atlantic pollock (Pollachius virens)

Origin of Carbon and Essential Fatty Acids in Higher Trophic Level Fish in Headwater Stream Food Webs

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