Fractionation of Oxygen Isotopes during Respiration

Lane, G.A.; Dole, Malcolm

doi:10.1126/science.123.3197.574

Cited by 144 publications

(69 citation statements)

References 15 publications

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“…Note, for clarity, the large number (~70) of measurements of 18  for human respiration are not shown (Epstein and Zeiri, 1988;Zanconato et al, 1992;Barkan and Luz, 2005). Data from Lane and Dole (1956) are not included, given the poor reproducibility of replicate measurements microbial experiments (1 of 6-12‰). 18  vs.  for wild type E. coli grown on glycerol from 15-37°C vs. mutants with only a single terminal O 2 reductase grown on glycerol at 37°C.…”

Section: Figures and Tablementioning

confidence: 99%

Effects of temperature and carbon source on the isotopic fractionations associated with O2 respiration for 17O/16O and 18O/16O ratios in E. coli

Stolper

Fischer

Bender

2018

Geochimica et Cosmochimica Acta

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Section: Figures and Tablementioning

confidence: 99%

Effects of temperature and carbon source on the isotopic fractionations associated with O2 respiration for 17O/16O and 18O/16O ratios in E. coli

Stolper

Fischer

Bender

2018

Geochimica et Cosmochimica Acta

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“…The units for ␦ are usually expressed in terms of parts/thousand or "parts/mil" (‰). For a process that transforms a substrate or source from one isotope composition (R S ) to a product with composition R P , the isotope effect or fractionation factor (␣) is as in Equation 3 (19).…”

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confidence: 99%

Differences in Carbon Isotope Discrimination of Three Variants of D-Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Reflect Differences in Their Catalytic Mechanisms

McNevin

Badger

Whitney

et al. 2007

Journal of Biological Chemistry

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The carboxylation kinetic (stable carbon) isotope effect was measured for purified D-ribulose-1,5-bisphosphate carboxylases/oxygenases (Rubiscos) with aqueous CO 2 as substrate by monitoring Rayleigh fractionation using membrane inlet mass spectrometry. This resulted in discriminations (⌬) of 27.4 ؎ 0.9‰ for wild-type tobacco Rubisco, 22.2 ؎ 2.1‰ for Rhodospirillum rubrum Rubisco, and 11.2 ؎ 1.6‰ for a large subunit mutant of tobacco Rubisco in which Leu 335 is mutated to valine (L335V). These ⌬ values are consistent with the photosynthetic discrimination determined for wild-type tobacco and transplastomic tobacco lines that exclusively produce R. rubrum or L335V Rubisco. The ⌬ values are indicative of the potential evolutionary variability of ⌬ values for a range of Rubiscos from different species: Form I Rubisco from higher plants; prokaryotic Rubiscos, including Form II; and the L335V mutant. We explore the implications of these ⌬ values for the Rubisco catalytic mechanism and suggest that Rubiscos that are associated with a lower ⌬ value have a less product-like carboxylation transition state and/or allow a decarboxylation step that evolution has excluded in higher plants.

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“…Isotope fractionation refers to the effects on chemical and physical properties produced by changes in mass. Indeed, small isotopic fractionation effects producing a difference in the order of 1.5% in favor of 160160 utilization as' compared to 180160 utilization have been reported in a number of biological systems (14). It might be expected that biological systems would then show preferential utilization of 180180 as compared to 160160.…”

Section: Discussionmentioning

confidence: 98%