Fractionation of Cerebellar Cells and Synapses: Certain Properties Related to Neurotransmission

Balázs, R.

doi:10.1007/978-3-642-68560-6_17

Cited by 2 publications

(1 citation statement)

References 87 publications

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“…When astroglial uptake was compared, HS subcultures were significantly different from primary culture and FCS subculture, both cultures. Cerebellar astrocytes have been shown to take up GABA, although less actively than glutamate (Balazs, 1982). Figure 6 shows the autoradiographic bright-field pictures of [3H]GABA uptake by neuronal-astroglial cultures, under the Same conditions as those used for the rate determinations.…”

Section: Gaba Uptakementioning

confidence: 99%

Plasticity of Astroglial Glutamate and γ‐Aminobutyric Acid Uptake in Cell Cultures Derived from Postnatal Mouse Cerebellum

Voisin

Viratelle

Girault

et al. 1993

Journal of Neurochemistry

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The plasticity of astroglial glutamate and gamma-aminobutyric acid (GABA) uptakes was investigated using mouse cerebellar cell cultures. The influence of external factors, such as different sera and/or the presence of neurons, was examined. Control autoradiography experiments showed that after short-term exposure to radioactive amino acids, granule cells took up neither glutamate nor GABA, and beta-alanine predominantly inhibited astroglial GABA uptake. Astroglial uptake was quantified by measuring the radioactivity taken up by the cells in the culture and relating this measurement to the number of glial fibrillary acidic protein-positive cells present. Glutamate uptake was investigated in astroglial cultures and subcultures and in neuronal-astroglial cultures derived from postnatal day 4 mouse cerebella. In the absence of neurons, glutamate uptake increased during the first 9 days after plating and then leveled off. At 14 days in vitro in horse serum, which favors the differentiation of fibrous-like astrocytes, glutamate uptake related to astrocyte number was twice as high as in fetal calf serum. In the presence of cerebellar neurons, this rate was even higher. The specificity of the responsiveness of astrocytes to neurons with respect to glutamate uptake was investigated by comparing GABA uptake in the different culture conditions. Neurons also increased the rate of GABA uptake by astrocytes. Another component of the astroglial plasma membrane, the density of beta-adrenergic receptors, was, however, not markedly affected by the presence of neurons. Hence, these results showed that in astrocytes plated from postnatal day 4 mouse cerebella, the level of neurotransmitter uptake can be regulated in vitro by factors present in sera and by cerebellar neurons in the culture. However, this plasticity declined during development because astrocytes plated from postnatal day 8 cerebella and cultured under identical conditions were less active in glutamate uptake and were insensitive to the presence of horse serum. The latter observation suggested that the metabolic plasticity of astrocytes is restricted to a period defined early in cerebellar development and is no longer evident by postnatal day 8.

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Section: Gaba Uptakementioning

confidence: 99%

Plasticity of Astroglial Glutamate and γ‐Aminobutyric Acid Uptake in Cell Cultures Derived from Postnatal Mouse Cerebellum

Voisin

Viratelle

Girault

et al. 1993

Journal of Neurochemistry

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Cholinergic innervation of the human cerebellum

Lacalle

Hersh

Saper

1993

J of Comparative Neurology

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Cholinergic innervation of the human cerebellum was investigated immunocytochemically by using a polyclonal rabbit antiserum against choline acetyltransferase. Immunoreactive structures were found throughout the cerebellar cortex but were localized predominantly in the vermis, flocculus, and tonsilla. These included 1) a population of Golgi cells in the granular layer; 2) a subpopulation of mossy fibers and glomerular rosettes; 3) thin, varicose fibers closely associated with the Purkinje cell layer and the molecular layer; and 4) a relatively dense network of fibers and terminals contributing to the glomerular formations in the granular layer. In the cerebellar nuclei, some cells stained positively for choline acetyltransferase, and a terminal field pattern could be detected with a distinct but sparse network of varicose fibers. Acetylcholine appears to be a primary transmitter in the vestibulocerebellar pathways at several levels, which may account for the potent effects of muscarinic antagonists in diminishing vestibular vertigo in humans.

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Fractionation of Cerebellar Cells and Synapses: Certain Properties Related to Neurotransmission

Cited by 2 publications

References 87 publications

Plasticity of Astroglial Glutamate and γ‐Aminobutyric Acid Uptake in Cell Cultures Derived from Postnatal Mouse Cerebellum

Plasticity of Astroglial Glutamate and γ‐Aminobutyric Acid Uptake in Cell Cultures Derived from Postnatal Mouse Cerebellum

Cholinergic innervation of the human cerebellum

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