Fractionation and Characterization of Three Main Components from Pennisetum sinese Roxb. (P. sinese) by Microwave-assisted H2O2-NaOH Extraction

Abstract

Fractionation of lignocellulosic components is a prerequisite for maximizing valorization of plant biomass in an integrated biorefinery. In this study, microwave-assisted H2O2-NaOH extraction was developed for fractionation of Pennisetum sinese Roxb. (P. sinese), a highly productive energy crop. Different reaction conditions including solid-liquid ratio, NaOH concentration, H2O2 concentration, temperature, and time were tested for their effects on P. sinese fractionation. The cellulose, hemicellulose, and lignin obtained under optimal fractionation conditions were characterized by Fourier transform infrared spectrometry (FT-IR) and nuclear magnetic resonance (NMR). The optimal fractionation conditions were a solid-liquid ratio of 1:25 using 0.5% H2O2 and 4% NaOH at 75 °C for 4 h, which gave cellulose, hemicellulose, and lignin yields of 42.8%, 21.9%, and 15.2%, respectively. FT-IR and NMR analyses of the fractionated components clearly confirmed their structural integrity and representation. The work demonstrates the potential of the alternative approach for efficient fractionation of lignocellulosic biomass components for further valorization.

Chemical and Biological Delignification of Biomass: A Review

“…293 Lignin yield increased with solid-to-liquid ratio and increasing alkaline dosage. 293 3.9. Ultrasound Assisted Extraction.…”

“…The yields of cellulose, hemicellulose, and lignin were 43 wt %, 22 wt %, and 15 wt %, respectively . Lignin yield increased with solid-to-liquid ratio and increasing alkaline dosage …”

“…Microwave NaOH and sulfuric acid pretreatments (at 453 K and 2 mol L –1 or 4 mol L –1 ) removed 86.5% to 91.6% of lignin from maize stems in 5 min . Tian et al optimized the microwave-assisted fractionation of lignocellulosic components from Pennisetum sinese with a solid-to-liquid ratio of 1:25 with 0.5 vol % H 2 O 2 and 4 wt % NaOH at 348 K for 4 h . The yields of cellulose, hemicellulose, and lignin were 43 wt %, 22 wt %, and 15 wt %, respectively .…”

“…290 Phragmites australis, commonly known as Reed, is composed of 23 wt % lignin�steam explosion followed by γvalerolactone microwave-assisted extraction achieved a delignification of 75 wt % at 473 K. 291 Microwave NaOH and sulfuric acid pretreatments (at 453 K and 2 mol L −1 or 4 mol L −1 ) removed 86.5% to 91.6% of lignin from maize stems in 5 min. 292 Tian et al optimized the microwave-assisted fractionation of lignocellulosic components from Pennisetum sinese with a solidto-liquid ratio of 1:25 with 0.5 vol % H 2 O 2 and 4 wt % NaOH at 348 K for 4 h. 293 The yields of cellulose, hemicellulose, and lignin were 43 wt %, 22 wt %, and 15 wt %, respectively. 293 Lignin yield increased with solid-to-liquid ratio and increasing alkaline dosage.…”

“…292 Tian et al optimized the microwave-assisted fractionation of lignocellulosic components from Pennisetum sinese with a solidto-liquid ratio of 1:25 with 0.5 vol % H 2 O 2 and 4 wt % NaOH at 348 K for 4 h. 293 The yields of cellulose, hemicellulose, and lignin were 43 wt %, 22 wt %, and 15 wt %, respectively. 293 Lignin yield increased with solid-to-liquid ratio and increasing alkaline dosage. 293 3.9.…”