Fractionate analysis of the phytochemical composition and antioxidant activities in advanced breeding lines of high-lycopene tomatoes

Abstract

This study investigates the antioxidant components [lycopene, total phenolics, total flavonoids, ascorbic acid (AsA) and dehydroascorbic acid (DHA)] as well as antioxidant activities of the hydrophilic and lipophilic fractions (AAHF and AALF) of peel, pulp and seed fractions isolated from red-ripe berries of the ordinary tomato cultivar Rio Grande and the two high-lycopene tomato breeding lines HLT-F61 and HLT-F62 simultaneously grown in an open-field of Northern Tunisia. Significant differences (p < 0.05) were found among cultivars for each trait studied. All fractions isolated from the red-ripe berries of HLT lines showed higher lycopene, total phenolics and total flavonoid contents, as well as higher AAHF and AALF, than those isolated from Rio Grande. Regardless of the fraction, HLT-F61 had the highest lycopene content (893.0 mg per kg fw, 280.0 mg per kg fw, and 47.5 mg per kg fw in peel, pulp and seed fractions, respectively) and total phenolics at least 2-fold and 3-fold higher than HLT-F62 and Rio Grande, respectively. Peel and seed fractions from HLT-F61 red-ripe tomato berries had the highest AsA content (345 mg per kg fw and 115 mg per kg fw, respectively), while no significant difference was found in the seed fraction between HLT-F62 and Rio Grande. The HLT-F62 pulp fraction showed the highest content of AsA (186 mg per kg fw) and DHA (151 mg per kg fw) among all the assayed cultivars. Except for the peel fraction, where HLT-F61 had similar AAHF values to HLT-F62, the high-lycopene line HLT-F61 showed higher AAHF values than HLT-F62 and Rio Grande. Regardless of the fraction, the highest AALF values were recorded in HLT-F61 berries. Thus, both HLT tomato lines are promising for the introduction, as advanced hybrids, in either fresh market or processing industry.

Influence of genotype and crop year on carotenoids content of peels from Bulgarian tomato cultivars

“…In the first crop year the highest contents of lycopene (97.16±0.81 mg/100g) and lutein (21.22±0.10 mg/100g) were found in the peels of the "Stella" cultivar of tomato and those of "Carobeta" cultivar had the highest content of beta-carotene (108.48±1.36 mg/100g), whereas in the second year of survey most of the lycopene (241.14±1.24 mg/100g) was contained in the "Aquarius F1" peels, and the beta-carotene (293.36±2.00 mg/100g) and lutein (13.58±0.15 mg/100g) in the "Stella" tomato peels. The results obtained for carotenoids content in the tomato peels (Table 2) were in agreement with data reprted from other reserches [9,10,14,15] and showed differences in carotenoids content for different tomato cultivars which may be due to the genotype features. Differences in carotenoids content among tomato cultivars has been previously reported [7,11].…”

Dynamic Changes in Health‐Promoting Properties and Eating Quality During Off‐Vine Ripening of Tomatoes

“…At the red ripe stage, "HLY13" and "HLY18" tomatoes exhibited the high-est level of β-carotene (19.8 and 19.3 mg/kg FW,respectively) indicating that, in these varieties, high lycopene amounts were associated with high β-carotene levels. Such contrasting differences between high-lycopene and ordinary tomato cultivars were ascribed to genotypic differences and growing conditions (Dumas et al, 2003;Ilahy et al, 2016Ilahy et al, , 2017Ilahy et al, , 2018. High-pigment tomato cultivars carry spontaneous mutations leading to exaggerated lightresponsiveness and deeply red-pigment mature fruits compared to ordinary cultivars (Atanassova, Stoeva-Popova, & Balacheva, 2007; Dynamic changes in health-promoting properties .…”

Antioxidant and anti-inflammatory effects of polyphenols extracted fromIlex latifoliaThunb

“…5 Synthetic antioxidants have been considered as endocrine disrupters or even carcinogenic agents, and interest in developing natural nutritional antioxidants has been increasing due to their welldocumented impact on human health. [6][7][8] Dietary supplements are becoming attractive alternatives as disease-preventive agents. Among the secondary metabolites of various plant species, polyphenols have been extensively studied because of their antioxidant, anti-cancer, anti-inammatory and antihyperglycemic effects in humans.…”