Fractional CO2 Laser Pretreatment Facilitates Transdermal Delivery of Two Vitamin C Derivatives

Abstract

Background: Topical vitamin C derivatives have been used to treat melasma and used as a skin whitener. The aim of this study was to compare skin histology and permeation of L-ascorbic acid 2-phosphate sesquimagnesium salt (MAP-1) and magnesium L-ascorbic acid-2-phosphate (MAP-2) after fractional CO 2 laser pretreatment. Methods: The effect of fractional laser treatment on porcine skin was examined by scanning electron microscopy and confocal laser scanning electron microscopy. The effect of fractional CO 2 laser treatment of different fluencies and pass numbers on transdermal flux of the two vitamin C derivatives through porcine skin was examined in vitro using a Franz diffusion chamber. Results: Fluxes of MAP-1 and MAP-2 across fractional CO 2 laser-treated (5 W) skin were eight-to 13-fold, and 20-to 22-fold higher, respectively, than the fluxes of these compounds across intact skin. Fluxes of MAP-1 and MAP-2 across fractional CO 2 laser-treated (9 W) skin were 14-to 19-fold, and 30-to 42-fold higher, respectively, than their fluxes across intact skin. Conclusion: Fractional CO 2 laser treatment is an effective way of delivering vitamin C derivatives into the skin.

Antera 3D camera: A novel method for evaluating the therapeutic efficacy of fractional CO2 laser for surgical incision scars

“…Based on the pursuit of the better appearance of incision scars, laser technology is applied in the treatment of scars, among which fractional CO 2 laser is a kind of common technology used most widely. It uses microwaves technique to generate a great deal of matrix‐arranged ultrashort pulsed beam, the advantages of which are as follows: (a) With strong penetrating ability, it can easily penetrate into the dermis to stimulate self‐repair process, resulting in more collagen rearrangement, as Hsiao found that fractional CO 2 laser can facilitate transdermal delivery of vitamin C for treating melasma; (b) More fast postinjury microvascular healing process, as laser wound will be healed in 3‐6 days, without seepage, bleeding, or infection; and (c) By customizing individualized treatment program according to different needs, the diameter of the pores and the depth of irradiation can be adjusted, and a single treatment often causes a visible effect. With the promotion of clinical application, the combined applications of a variety of scar restraining means are proposed to obtain better therapeutic results …”

Advances in fractional technology for skin rejuvenation, skin tightening, drug delivery, and treating scars and skin defects

“…Most preclinical studies have demonstrated an increased flux and accumulation of the drug with increasing numbers of passes. [127][128][129][130] Four passes of a fractional CO2 laser showed an equivalent flux to that achieved with a fully ablative laser with the ex vivo application of both tranexamic acid and ascorbic acid 2-glucoside on porcine skin. 127,129 Vitamin C derivatives have been shown to have a 13-to 42-fold higher transdermal flux across porcine skin after fractional CO2 pretreatment, depending on the fluence and the particular derivative.…”

“…127,129 Vitamin C derivatives have been shown to have a 13-to 42-fold higher transdermal flux across porcine skin after fractional CO2 pretreatment, depending on the fluence and the particular derivative. 130 A single fractional CO2 treatment increased the flux of tretinoin by 9-fold in mouse skin. 128 In general, AFLs produce increased transdermal flux of active agents in comparison with NAFLs.…”

“…125,126 Drugs found to have a successful permeation with AFLs include tranexamic acid, tretinoin, multiple vitamin C derivatives, autologous cells, and PRP. [127][128][129][130][131][132] When used for drug delivery, laser fluences are typically lower than those used for skin rejuvenation. Most preclinical studies have demonstrated an increased flux and accumulation of the drug with increasing numbers of passes.…”

The Applications of Fractional Lasers in Taiwan

“…Another fractional 10,600nm CO2 laser (150XJ, Sharplan Laser Inc., Yokneam, Israel) for transdermal absorption of ascorbic acid 2-glucoside (AA2G) was researched by Hsiao, et al, with the setting of 5-9 W, MTZ 150μm, and 2% coverage for a total of four passes [3]. The percutaneous absorption of L-ascorbic acid 2-phosphate sesquimagnesium salt (MAP-1) was 8 to 13-fold increased, while that of magnesium L-ascorbic acid-2-phosphate (MAP-2) was 20 to 22-fold higher after 5W irradiation by the fractional CO2 laser.…”