Fractalkine/CX3CR1 induces apoptosis resistance and proliferation through the activation of the AKT/NF‐κB cascade in pancreatic cancer cells

Wang, Hui; Cai, Jin; Du, Shaoxia; Guo, Zhongkui; Xin, Baoping; Wang, Juan; Wei, Wei; Shen, Xiaohong

doi:10.1002/cbf.3278

Cited by 40 publications

(27 citation statements)

References 28 publications

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“…The apoptosis of MDA-MB-231 and HCC1937 cells treated with sh-NC, sh-PUF60, OE-NC, or OE-PUF60 was determined by flow cytometry assay with Annexin V (FITC)/propidium iodide (PI) apoptosis detection kit (KeyGEN Biotech, Jiangsu, China) according to previous study 19…”

Section: Methodsmentioning

confidence: 99%

PUF60 accelerates the progression of breast cancer through down-regulation of PTEN expression

Sun

Lei

Hou

et al. 2019

CMAR

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BackgroundPUF60 is a splicing variant of far upstream element binding protein 1-interacting repressor, which is abnormally expressed in a variety of tumors and is closely involved in their progression. However, whether PUF60 participates in the occurrence and development of breast cancer remains unknown. Therefore, the objective of the current study is to explore the effects and mechanism of PUF60 in the progression of breast cancer.MethodsPUF60 expression patterns in breast cancer tissues and cells were determined by RT-PCR and Western blotting. The relationship between PUF60 expression and patients’ clinical features and outcome was evaluated to assess the potential of PUF60 as a marker for progression and prognosis prediction. CCK-8, flow cytometry, transwell and in vivo tumor formation assays were used to detect cell proliferation, apoptosis, migration, invasion and tumorigenesis. The effects of PUF60 on the activation of PTEN/PI3K/AKT were also evaluated by Western blotting and immunofluorescence assays.ResultsThe expression of PUF60 was elevated in breast cancer tissue samples and cell lines, and its high expression was closely associated with the high incidence of lymph node metastasis and advanced TNM stage. Besides, upregulation of PUF60 with lentivirus infection significantly increased the growth, migration, and invasion and repressed the apoptosis of breast cancer HCC1937 and MDA-MB-231 cells, while silencing of PUF60 with shRNA showed the opposite results. Moreover, PUF60 upregulation promoted the expression of p-AKT, PI3K, and mTOR, while decreased PTEN expression through inhibiting its stability and enhancing its ubiquitination. Furthermore, upregulation of PUF60 promoted the tumorigenesis in vivo, whereas this effect was impaired when PTEN expression was upregulated in MDA-MB-231 and HCC1937 cells.ConclusionThis study demonstrates that PUF60 is highly expressed in breast cancer; upregulation of PUF60 accelerates the progression of breast cancer through PTEN inhibition.

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Section: Methodsmentioning

confidence: 99%

PUF60 accelerates the progression of breast cancer through down-regulation of PTEN expression

Sun

Lei

Hou

et al. 2019

CMAR

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“…In another PNI tumor, CCA, studies showed that NF-κB signaling could be activated by the CCL5/CCR5 axis through increasing the expression of MMP2 and MMP9, thus contributing to the invasion and metastasis of tumor cells, which could be inhibited by CCL5 neutralizing antibody or CCR5 inhibitor maraviroc (94). Wang et al (95) found that CX3CL1/CX3CR1 could promote the invasion of pancreatic cancer through the activation of the NF-κB signaling pathway (Fig. 3).…”

Section: Reciprocal Interaction Between Other Factors and Chemokines mentioning

confidence: 98%

Functions of chemokines in the perineural invasion of tumors (Review)

et al. 2018

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The perineural invasion (PNI) of malignant tumors is a form of tumor progression in which cancer cells encroach along nerves. PNI hinders curative resection. Residual tumor cells in or around nerves can bring about local recurrence, infiltration and metastasis. This behavior is usually associated with a poor clinical prognosis. Therefore, it is necessary to investigate novel ligand-receptor crosstalk between nerves and tumor cells that promote the process of PNI. Chemokines are regarded as one of pivotal factors involved in the process of PNI. The present review collates information provided by previous studies with regard to the role of chemokines in PNI. The study presents a definition of PNI in cancer, generalizes the biological characteristics and the expression of chemokines and their receptors in cancer types associated with PNI, and discusses the underlying molecular mechanisms of chemokines, the reciprocal interactions between chemokines and other factors in PNI, and the interconnectivity of the microenvironment and chemokines. The aim of the review is to thoroughly illustrate the molecular cues of chemokines in cancer with PNI and to identify novel antitumor targets.

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“…Importantly, chemokines can promote tumor cell survival by regulating the balance between NF-κB-associated pro-and antiapoptosis proteins. CCR5 and CX3CL1 were demonstrated to promote the expression of antiapoptotic and tumor-promoting proteins such as Bcl-xl, Bcl-2, and C-IAP1, as well as to reduce the expression of apoptotic proteins including cleaved caspase-3 and -9, PARP, and Bax via the NF-κB pathway [99,100].…”

Section: Roles Of Chemokines In Tumor Growth and Proliferationmentioning

confidence: 99%

“…Furthermore, in HCC patients, CX3CL1/CX3CR1 regulates the cancer cell cycle and tumor progression via the autocrine or paracrine systems, which is associated with positive outcomes [211]. In contrast, CX3CL1/CX3CR1 has been proven to induce tumor growth, proliferation, and apoptosis resistance through activating the AKT/NF-κB [100] and JAK/STAT signalling pathways in PDAC [212]. Therefore, a study in PDAC patients showed evidence that high expression of the CX3CL1/CX3CR1 axis in tumor tissues led to a poor prognosis for OS [213].…”

Section: Cx3cl1/cx3cr1mentioning

confidence: 99%

Chemokines and their Receptors: Multifaceted Roles in Cancer Progression and Potential Value as Cancer Prognostic Markers

Thu

Lee

Cho

2020

Cancers

148

131

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Chemokines are chemotactic cytokines that mediate immune cell chemotaxis and lymphoid tissue development. Recent advances have indicated that chemokines and their cognate receptors play critical roles in cancer-related inflammation and cancer progression. On the basis of these findings, the chemokine system has become a new potential drug target for cancer immunotherapy. In this review, we summarize the essential roles of the complex network of chemokines and their receptors in cancer progression. Furthermore, we discuss the potential value of the chemokine system as a cancer prognostic marker. The chemokine system regulates the infiltration of immune cells into the tumor microenvironment, which induces both pro- and anti-immunity and promotes or suppresses tumor growth and proliferation, angiogenesis, and metastasis. Increasing evidence indicates the promising prognostic value of the chemokine system in cancer patients. While CCL2, CXCL10, and CX3CL1/CX3CR1 can serve as favorable or unfavorable prognostic factors depending on the cancer types, CCL14 and XCL1 possess good prognostic value. Other chemokines such as CXCL1, CXCL8, and CXCL12 are poor prognostic markers. Despite vast advances in our understanding of the complex nature of the chemokine system in tumor biology, knowledge about the multifaceted roles of the chemokine system in different types of cancers is still limited. Further studies are necessary to decipher distinct roles within the chemokine system in terms of cancer progression and to validate their potential value in cancer prognosis.

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Fractalkine/CX3CR1 induces apoptosis resistance and proliferation through the activation of the AKT/NF‐κB cascade in pancreatic cancer cells

Cited by 40 publications

References 28 publications

PUF60 accelerates the progression of breast cancer through down-regulation of PTEN expression

PUF60 accelerates the progression of breast cancer through down-regulation of PTEN expression

Functions of chemokines in the perineural invasion of tumors (Review)

Chemokines and their Receptors: Multifaceted Roles in Cancer Progression and Potential Value as Cancer Prognostic Markers

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