FOXP3 and IL-10 overexpression: A novel diagnostic biomarker in Iraqi patients having hyperthyroidism treated with radioactive iodine

The study conducted at Center for the Early Detection of Breast Tumors at Medical City’s oncology teaching hospital carried 90 patients who participated in this study. The research was carried out between February 15, 2021, and July 20, 2021. The diagnosis was made by the consultant medical team using the triple assessment technique, which includes physical breast examination, ultrasonography, mammography, and fine needle aspiration cytology. Women patients clasified into 3 groups (benign tumor, control and malignant tumor) benign B (34 women) divided in/to two sub groups such as (benign premenopausal age B1 (17) and benign postmenopausal age B2 (17) and malignant M (34), malignant premenopausal age M1 (17) and malignant postmenopausal age M2 (17), and control group C includes (11) premenopausal age C1 and (11) C2. An ELISA approach was used to measure the level of soluble PDL, PD L1, and CD8 expression in serum. PD1 shows significant increase (p ≤ 0.05) in pre- and post-menopausal malignant groups M5, M6 when compared with C1, C2, B1, B2. PDL-1 levels were non-significant (p ≥ 0.05) in pre and postmenopausal control and benign groups (C1, C2, B1 and B2) while they were increased significantly (p ≤ 0.05) in M1, M2 in comparison with B2,C1,C2, B1. CD8 in the M1 increased significantly (p ≤ 0.05) when compere with group1, group 2, group bening1, group benign 2 and malignant 2. Conclusions from the current study that the values of PD1and PDL1in pre and postmenopausal malignant women breast cancer showed an increase compere with the other groups, the levels of CD8+ increase in the premenopausal malignant breast cancer women in comparison with other groups.

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“…Several other drugs that target PD-1 or PD-L1 have demonstrated notable response rates in clinical trials in a variety of tumor types. 17,18…”

Section: Resultsmentioning

confidence: 99%

Updating of PD-1, PDL-1 and CD8+ in Benign and Malignant Breast Cancer in Iraqi Women

Hameedi¹,

Sultan²,

Mahdi³

2022

IJDDT

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“…qRT-PCR was performed using a Real-time PCR machine that had been preconfigured with qPCR soft software before the experiment began (BioMolecular, Australia). By employing the threshold cycle (Ct) approach, the relative levels of gene expression as well as the fold changes that occurred were determined (10). There were two identical reactions that were carried out.…”

Section: Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

IL-38 gene expression: A new player in Graves’ ophthalmopathy patients in Iraq

Jawad¹,

Numan²,

Ahmed³

et al. 2023

Biomedicine

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Introduction and Aim: Thyroid eye illness is widely recognized as one of the most significant concerns confronting the medical profession today. The thyroid-related ophthalmopathy also known as Graves’ ophthalmopathy is an autoimmune disorder that cannot be reversed and hence, research pertaining to the identification of novel markers that can explain both the prognosis and the recovery of the condition is needed. In this study, we aimed to investigate the IL-38 gene expression levels among Graves’ ophthalmopathy patients in Iraq. Methodology: The TSH levels were measured with an enzyme-linked immunosorbent assay (ELISA). The level of IL-38 gene expression in patient blood samples and normal healthy controls was measured qualitatively using RT-PCR. Results: Patients who were diagnosed with Graves’ ophthalmopathy were observed to have abnormally low levels of thyrotropin (TSH) in their serum. In these patients, the interleukin IL-38 gene expression was observed to be significantly greater compared to healthy controls (P<0.01), the tendency of which continued even after one year of therapy with anti-thyroid drugs. Conclusion: The findings of this study indicate that the IL-38 transcript is important in the autoimmune response. The identification of IL-38 expression levels could contribute in the early clinical diagnosis and treatment of thyroid eye disease

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