FOXO3a Gene Polymorphism Associated with Asthma in Indian Population

Barkund, Shravani; Shah, Tejas; Ambatkar, Nikhil; Gadgil, Maithili S.; Joshi, Kalpana

doi:10.1155/2015/638515

Cited by 20 publications

(25 citation statements)

References 23 publications

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“…10 The association between FOXO3a polymorphisms and different types of inflammatory diseases as idiopathic pulmonary fibrosis, lung cancer, bronchiolitis, Crohn's disease, rheumatoid arthritis and Sjogren's syndrome has been demonstrated in previous studies. [16][17][18]23,24 To our knowledge, the association between FOXO3a gene polymorphism and asthma has only been explored in two previous studies 19,20 . Herein, we focused on exploring the association between FOXO3a gene polymorphism and asthma, atopy and asthma severity in Egyptian population.…”

Section: Discussionmentioning

confidence: 99%

“…Generally, CT genotype represented the highest prevalent genotype in both patients and controls while the TT genotype was the least. On the other hand, Barkund et al (2015) determined that in their asthmatic patients, the TT mutant genotype had the highest frequency (51.75%) and the T mutant allele showed the highest prevalence (71%). 19 Similar results were shown by Amarin et al (2017) where the frequency of mutant TT genotype was the highest (49%) and the mutant T allele was present in 64% of asthmatics.…”

Section: Discussionmentioning

confidence: 99%

“…Genotyping of FOXO3a polymorphism was performed using PCR-RFLP technique. The primer sequences used were as follows: Forward Primer 5′-CTC CTT GGT CAG TTT GGT G 3′; Reverse Primer: 5′-ATG AGT GAA GAT GGA AGT AAG C -3′ 19 . Amplification was performed using 2X PCR master mix solution (iTaqTM) (iNtRON biotechnology, Korea) in total volume of 20μl.…”

Section: Detection Of Foxo3a Polymorphism Using Pcr-rflpmentioning

confidence: 99%

“…However, studies in our Egyptian population are still lacking. Recently, a significant association between the FOXO3a single-nucleotide polymorphism (rs13217795) and susceptibility to asthma and was described by Barkund et al (2015) and Amarin et al (2017). 19,20 Furthermore, the study by Barkund et al (2015) revealed that FOXO3a SNP (rs13217795) is significantly associated with asthmatics plausibly contributing to the hyperactivity of T cells, neutrophils, and mast cells, increased production of proinflammatory cytokines, and downregulation of anti-inflammatory cytokines 19 .…”

Section: Introductionmentioning

confidence: 99%

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FOXO3a gene polymorphism and bronchial asthma in Egyptian children

Elrifai

Al-Wakeel

Osman

et al. 2019

Egypt J Pediatr Allergy Immunol

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Background: FOXO3a proteins play multiple crucial roles in immune response. FOXO3 inhibits T cell proliferation, induces T cell apoptosis via upregulation of proapoptotic proteins and it suppresses T cell activation preventing autoimmunity. The role of FOXO3a gene in the pathogenesis of bronchial asthma has been studied in few ethnic groups and revealed its implication in asthma pathogenesis. Objectives: The aim of the current study is to detect the association between single nucleotide polymorphism of the FOXO3a gene (rs13217795) and bronchial asthma, atopy and asthma severity in Egyptian children. Methods: The current cross-sectional case-control study was performed on 75 asthmatic children aged 2 to 12 years following up in the pulmonology outpatient clinic in Children's hospital, Cairo University and 75 age and sex matched healthy controls. Candidates were subjected to clinical evaluation in addition to genotyping for the FOXO3a gene polymorphism using PCR-RFLP technique. Results: The highest frequency was for the heterozygous type CT in both cases and controls groups. The genotype frequencies of mutant type TT for cases and controls were 12 % and 16% respectively, and the T allele frequencies were 37.2% in cases and 46.7% in the control group while CC genotype was present in 37.3% of asthmatic patients and 22.6% in the controls and the C allele was detected in 62.8% and 53.3% for cases and controls respectively. No statistically significant differences were observed between asthmatic patients and controls regarding the different genotypes of the FOXO3a gene polymorphism (p=0.161). No significant association was detected between the different genotypes of the FOXO3a gene polymorphism and the atopic status (p=0.536) or the different grades of asthma severity (p= 0.545). Conclusions: The study of FOXO3a gene polymorphism (rs13217795) in asthmatic Egyptian children revealed low frequency of the mutant TT genotype among cases and controls. In the current study, FOXO3a polymorphism has no role in the pathogenesis of asthma or atopy. Moreover, it has no relation to degree of disease severity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Detection Of Foxo3a Polymorphism Using Pcr-rflpmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

FOXO3a gene polymorphism and bronchial asthma in Egyptian children

Elrifai

Al-Wakeel

Osman

et al. 2019

Egypt J Pediatr Allergy Immunol

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“…The C>T allele at rs13217795 in FOXO3a was found to be associated with asthma in an Indian population. In fact, the risk for asthma development in a population with the CT allele was found to be 5.54 times higher and those with the CC genotype and 21.45 times higher than those with the TT allele (Barkund et al, 2015)…”

Section: Introductionmentioning

confidence: 99%

Immune-related gene polymorphisms in pulmonary diseases

et al. 2017

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Between the DNA sequences of two randomly-selected human genomes, which consist of over 3 billion base pairs and twenty five thousand genes, there exists only 0.1% variation and 99.9% sequence identity. During the last couple of decades, extensive genome-wide studies have investigated the association between single-nucleotide polymorphisms (SNPs), the most common DNA variations, and susceptibility to various diseases. Because the immune system's primary function is to defend against myriad infectious agents and diseases, the large number of people who escape serious infectious diseases underscores the tremendous success of this system at this task. In fact, out of the third of the global human population infected with Mycobacterium tuberculosis during their lifetime, only a few people develop active disease, and a heavy chain smoker may inexplicably escape all symptoms of chronic obstructive pulmonary disease (COPD), lung cancer, and other smoke-associated lung diseases. This may be attributable to the genetic makeup of the individual(s), including their SNPs, which provide some resistance to the disease. Pattern recognition receptors (PRRs), transcription factors, cytokines and chemokines all play critical roles in orchestrating immune responses and their expression/activation is directly linked to human disease tolerance. Moreover, genetic variations present in the immune-response genes of various ethnicities may explain the huge differences in individual outcomes to various diseases and following exposure to infectious agents. The current review focuses on recent advances in our understanding of pulmonary diseases and the relationship of genetic variations in immune response genes to these conditions.

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Analysis of FOXO3a Gene Polymorphism Associated with Asthma

Shah

Joshi

2018

FOXO Transcription Factors

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FOXO3a Gene Polymorphism Associated with Asthma in Indian Population

Cited by 20 publications

References 23 publications

FOXO3a gene polymorphism and bronchial asthma in Egyptian children

FOXO3a gene polymorphism and bronchial asthma in Egyptian children

Immune-related gene polymorphisms in pulmonary diseases

Analysis of FOXO3a Gene Polymorphism Associated with Asthma

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