FoxO directly regulates the expression of TOR/S6K and vitellogenin to modulate the fecundity of the brown planthopper

Dong, Yi; Chen, Weiwen; Kang, Kui Dong; Pang, Rui; Dong, Yipei; Li, Kai; Zhang, Wenqing

doi:10.1007/s11427-019-1734-6

Cited by 18 publications

(22 citation statements)

References 53 publications

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“…In this study, our results indicated that the expression levels of genes ( SlPI3K , SlAkT , SlTOR , SlS6K and SlCREB ) were increased but the SlFoxO gene expression was reduced when the insulin pathway was activated by bovine insulin, while the transcriptions were exactly inverse when the SlInR gene was knocked down ( Figure 1 B and Figure 2 B). Similar results were observed in many other insects, such as Nilaparvata lugens [ 51 ] and Blattella germanica [ 53 ]. However, a recent report opposed this, as the expression of FoxO was reduced when the InR gene was knocked down in B. dorsalis [ 44 ].…”

Section: Discussionsupporting

confidence: 87%

“…Then, the phosphoinositide 3-kinase (PI3K)-serine/threonine kinase (Akt) signaling pathway is activated, in turn [ 21 , 22 ]. Further, the downstream genes ( TOR , FoxO , CREB , S6K ) will be activated or have a suppressed response to this process [ 28 , 50 , 51 , 52 ]. In this study, our results indicated that the expression levels of genes ( SlPI3K , SlAkT , SlTOR , SlS6K and SlCREB ) were increased but the SlFoxO gene expression was reduced when the insulin pathway was activated by bovine insulin, while the transcriptions were exactly inverse when the SlInR gene was knocked down ( Figure 1 B and Figure 2 B).…”

Section: Discussionmentioning

confidence: 99%

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Effect of Insulin Receptor on Juvenile Hormone Signal and Fecundity in Spodoptera litura (F.)

Pan

Pei

Zhang

et al. 2022

Insects

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Insulin signaling can regulate various physiological functions, such as energy metabolism and reproduction and so on, in many insects, including mosquito and locust. However, the molecular mechanism of this physiological process remains elusive. The tobacco cutworm, Spodoptera litura, is one of the most important pests of agricultural crops around the world. In this study, phosphoinositide 3-kinase (SlPI3K), protein kinase B (SlAKT), target of rapamycin (SlTOR), ribosomal protein S6 kinase (SlS6K) and transcription factor cAMP-response element binding protein (SlCREB) genes, except transcription factor forkhead box class O (SlFoxO), can be activated by bovine insulin injection. Then, we studied the influence of the insulin receptor gene (SlInR) on the reproduction of S. litura using RNA interference technology. qRT-PCR analysis revealed that SlInR was most abundant in the head. The SlPI3K, SlAKT, SlTOR, SlS6K and SlCREB genes were decreased, except SlFoxO, after the SlInR gene knockdown. Further studies revealed that the expression of vitellogenin mRNA and protein, Methoprene-tolerant gene (SlMet), could be down-regulated by the injection of dsRNA of SlInR significantly. Furthermore, a depletion in the insulin receptor by RNAi significantly decreased the content of juvenile hormone III (JH-III), total proteins and triacylgycerol. These changes indicated that a lack of SlInR could impair ovarian development and decrease fecundity in S. litura. Our studies contribute to a comprehensive insight into reproduction, regulated by insulin and the juvenile hormone signaling pathway through nutrition, and a provide theoretical basis for the reproduction process in pest insects.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Effect of Insulin Receptor on Juvenile Hormone Signal and Fecundity in Spodoptera litura (F.)

Pan

Pei

Zhang

et al. 2022

Insects

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“…When secreted into the host rice plant, Vitellogenin (Vg), regulated by NlFoxO (Dong et al, 2021), can directly interact with the rice transcription factor OsWRKY71, thus effectively attenuating H 2 O 2 -mediated plant defense and improving insect feeding performance (Ji et al, 2021). Nevertheless, the effector perceived by NLRs or PRRs has not been identified in insects.…”

Section: Rice Insect Effectorsmentioning

confidence: 99%

Rice functional genomics: decades’ efforts and roads ahead

Chen

Deng

Ding

et al. 2021

Sci. China Life Sci.

139

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Rice (Oryza sativa L.) is one of the most important crops in the world. Since the completion of rice reference genome sequences, tremendous progress has been achieved in understanding the molecular mechanisms on various rice traits and dissecting the underlying regulatory networks. In this review, we summarize the research progress of rice biology over past decades, including omics, genome-wide association study, phytohormone action, nutrient use, biotic and abiotic responses, photoperiodic flowering, and reproductive development (fertility and sterility). For the roads ahead, cutting-edge technologies such as new genomics methods, high-throughput phenotyping platforms, precise genome-editing tools, environmental microbiome optimization, and synthetic methods will further extend our understanding of unsolved molecular biology questions in rice, and facilitate integrations of the knowledge for agricultural applications.

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“…Based on our previous results [46,48], we measured the fecundity of dsRNA-injected BPHs within the first 7 days to evaluate the effect on BPH fertility of dsRNA administration. Briefly, awakened female adults treated with dsRNA were paired with two untreated males and transferred to the rice plants described above.…”

Section: Bioassaysmentioning

confidence: 99%

Genome-Wide Analysis of the Amino Acid Auxin Permease (AAAP) Gene Family and Identification of an AAAP Gene Associated with the Growth and Reproduction of the Brown Planthopper, Nilaparvata lugens (Stål)

Yue

Pang

Tian

et al. 2021

Insects

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Amino acids play a vital role in several biological processes in organisms and are mainly acquired through diet by most insects. The amino acid auxin permease (AAAP) transporter family is an important amino acid transporter gene family in insects for the transportation of amino acids into and out of cells across the plasma membrane. Here, we identified 21 putative AAAP family members in the genome of the brown planthopper (BPH), Nilaparvata lugens, a devastating pest that feeds only on the phloem sap of rice plants. Molecular characteristic analysis indicated large variations in protein features and amino acid sequences among the predicted AAAP family members in BPH. Phylogenetic analysis clustered these AAAP transporters into three subgroups, with the members in the same group sharing a similar pattern of conserved motif distribution. Through ortholog gene recognition and spatiotemporal gene expression analysis, the AAAP gene NlAAAP07, which was predicted to regulate BPH larval growth and female fecundity, was identified. RNA interference (RNAi)-mediated suppression of NlAAAP07 significantly postponed the duration of 3rd instar nymphs developing into adults from 7.4 days to 9.0 days, and decreased the oviposition amount and egg hatching rate of females by 30.7% and 11.0%, respectively. Our results provide a foundation for further functional analysis of AAAP transporters in BPH.

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FoxO directly regulates the expression of TOR/S6K and vitellogenin to modulate the fecundity of the brown planthopper

Cited by 18 publications

References 53 publications

Effect of Insulin Receptor on Juvenile Hormone Signal and Fecundity in Spodoptera litura (F.)

Effect of Insulin Receptor on Juvenile Hormone Signal and Fecundity in Spodoptera litura (F.)

Rice functional genomics: decades’ efforts and roads ahead

Genome-Wide Analysis of the Amino Acid Auxin Permease (AAAP) Gene Family and Identification of an AAAP Gene Associated with the Growth and Reproduction of the Brown Planthopper, Nilaparvata lugens (Stål)

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