Fowlpox Virus Host Range Restriction: Gene Expression, DNA Replication, and Morphogenesis in Nonpermissive Mammalian Cells

Somogyi, P; Frazier, Judith A.; Skinner, Michael A.

doi:10.1006/viro.1993.1608

Cited by 106 publications

(56 citation statements)

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“…33,34 Although replication-competent vaccinia virus vectors have been used in cancer immunotherapy trials in humans, [45][46][47][48] their use is undesirable due to the risks of skin and central nervous system complications and potential spread to unvaccinated contacts. [29][30][31] Such risks are particularly acute in adjuvant cancer immunotherapy protocols, where patients may be in an immunocompromised state because of their disease or primary therapy.…”

Section: Figure 7 Human DC Infected With Fowlpox Virus Retain Viabilimentioning

confidence: 99%

“…The host range restriction may not be so complete for MVA as for FWPV. 34,50 Moreover, vaccinia viruses encode a battery of gene products which are involved in immune evasion. 37,38 While it may be anticipated that FWPV also contains similar immune evasion genes, such as the SER-PIN gene homologue SPI-3, 51 their functions may be compromised in a heterologous host.…”

Section: Figure 7 Human DC Infected With Fowlpox Virus Retain Viabilimentioning

confidence: 99%

“…However, they can initiate an abortive infection in mammalian cells, Gene Therapy demonstrating early and in some cases late gene expression without production of viable progeny. 27,33,34 Immunisation of mice with recombinant FWPV expressing LacZ has been shown to protect mice from challenge against a tumour line which had been transfected with the LacZ gene. 35 In this study we used a murine model system to investigate whether recombinant FWPV could infect DC and stimulate an MHC class I restricted immune response.…”

Section: Introductionmentioning

confidence: 99%

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Dendritic cells infected with recombinant fowlpox virus vectors are potent and long-acting stimulators of transgene-specific class I restricted T lymphocyte activity

et al. 2000

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The identification of dendritic cells (DC) as the major antigen-presenting cell type of the immune system, combined with the development of procedures for their ex vivo culture, has opened possibilities for tumour immunotherapy based on the transfer of recombinant tumour antigens to DC. It is anticipated that the most effective type of response would be the stimulation of specific, MHC class I restricted cytotoxic T lymphocytes capable of recognising and destroying tumour cells. In order to make this approach possible, methods must be developed for the transfer of recombinant antigen to the DC in such a way that they will initiate an MHC class I restricted response. Here, we demonstrate that murine DC infected with a recombinant fowlpox virus (rFWPV) vector stimulate a powerful, MHC class I restricted response against a recombinant antigen. A rFWPV containing the OVA gene was constructed and used to infect the DC line

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Section: Figure 7 Human DC Infected With Fowlpox Virus Retain Viabilimentioning

confidence: 99%

Section: Figure 7 Human DC Infected With Fowlpox Virus Retain Viabilimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dendritic cells infected with recombinant fowlpox virus vectors are potent and long-acting stimulators of transgene-specific class I restricted T lymphocyte activity

et al. 2000

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“…It has been reported that replication of this poxvirus is blocked in mammalian cells Somogyi et al, 1993). Recombinant fowlpox viruses have been used in expression of rabies virus glycoprotein , tumour-associated antigen (Wang et al, 1995) and cytokines (Leong et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Expression of target genes by coinfection with replication-deficient viral vectors.

Yap

Ishii

Aizaki

et al. 1998

Journal of General Virology

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An in vivo transcription system was developed by coinfection of cells with replication-deficient viral vectors. Recombinant baculovirus (AcT7HCVLuc) and fowlpox virus (FPVT7HCVLuc) carrying a cDNA of the hepatitis C virus (HCV) minigene encoding the HCV 5h untranslated region (UTR), a luciferase gene and the 3h UTR, including the 98 nt extra sequence, under the control of the T7 promoter were constructed. The HCV minigene was synthesized in various cells by coinfection with one of these two viruses and recombinant baculovirus (AcCAT7) or adenovirus (AdexCAT7) expressing T7 RNA polymerase under the control of a mammalian promoter. Only a low level of luciferase expression was ob-

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“…In mammalian cells FPV replication is abortive with no evidence of production of infectious virus, though late CPE are observed at high m.o.i. (Somogyi et al, 1993). The point at which replication is blocked appears to depend on cell type rather than on species.…”

mentioning

confidence: 99%

Expression of bacteriophage T7 RNA polymerase in avian and mammalian cells by a recombinant fowlpox virus

Britton¹,

Green²,

Kottier³

et al. 1996

Journal of General Virology

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The bacteriophage T7 RNA polymerase gene was integrated into the fowlpox virus genome under the control of the vaccinia virus early/late promoter, P7.5-The recombinant fowlpox virus, fpEFLT7pol, stably expressed T7 RNA polymerase in avian and mammalian cells, allowing transient expression of transfected genes under the control of the T7 promoter. The recombinant fowlpox virus expressing T7 RNA polymerase offers an alternative to the widely used vaccinia virus vTF7-3, or the recently developed modified vaccinia virus Ankara (MVA) T7 RNA polymerase recombinant, a highly attenuated strain with restricted host-range. Recombinant fowlpox viruses have the advantage that as no infectious virus are produced from mammalian cells they do not have to be used under stringent microbiological safety conditions. Recombinant vaccinia viruses (rVV) have been produced expressing a variety of bacteriophage DNA-dependent RNA polymerases: T7 RNA polymerase (Fuerst et al., 1986), T3 RNA polymerase (Rodriguez et al., 1990) and SP6 RNA polymerase (Usdin et al., 1993). Cells infected with these rVVs can express biologically active products transiently from genes under the control of the appropriate RNA polymerase promoter. The most utilized system is an rVV expressing T7 RNA polymerase, vTF7-3. Translation of the T7 transcripts was inefficient, as only 5-10 % were capped, but inclusion of an internal ribosome entry signal (IRES) sequence, from encephalomyocarditis virus, at the 5' end of the T7-derived RNA transcripts, resulted in mRNAs that were CAP-independent and efficiently translated (Elroy-Stein et al., 1989).In addition to transient expression, a gene under the control of the T7 RNA polymerase promoter can also be integrated into the genome of a second rVV allowing high level expression of the gene product in cells dually infected with vTF7-3 and the second rVV (Fuerst et al., 1987). The vTF7-3 system has been utilized for the expression of RNA per se, e.g. a defective RNA (D-

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Fowlpox Virus Host Range Restriction: Gene Expression, DNA Replication, and Morphogenesis in Nonpermissive Mammalian Cells

Cited by 106 publications

References 0 publications

Dendritic cells infected with recombinant fowlpox virus vectors are potent and long-acting stimulators of transgene-specific class I restricted T lymphocyte activity

Dendritic cells infected with recombinant fowlpox virus vectors are potent and long-acting stimulators of transgene-specific class I restricted T lymphocyte activity

Expression of target genes by coinfection with replication-deficient viral vectors.

Expression of bacteriophage T7 RNA polymerase in avian and mammalian cells by a recombinant fowlpox virus

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