Fourth International Workgroup on Genotoxicity testing: Results of the in vivo Comet assay workgroup

Burlinson, Brian; Tice, Raymond R.; Speit, Günter; Agurell, Eva; Brendler‐Schwaab, Susanne; Collins, Andrew; Escobar, Patricia; Honma, Masamitsu; Kumaravel, T. S.; Nakajima, Masahiro; Sasaki, Yu; Thybaud, Véronique; Uno, Yoshifumi; Vasquez, Marie; Hartmann, Andreas

doi:10.1016/j.mrgentox.2006.08.011

Cited by 472 publications

(251 citation statements)

References 24 publications

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“…The images of 100 randomly chosen nuclei (50 cells from each of two replicate slides) were analysed visually by manual scoring 14 ( Figure 1). Each image was classified according to the intensity of the fluorescence in the comet tail and was given a value of either of 0, 1, 2, 3, or 4 (from undamaged class 0 to maximally damaged class 4), so that the total scores of slide could be between 0 and 400 arbitrary units (AU).…”

Section: Hundred Cells Were Scored and The Number Of Viable Cells (Shmentioning

confidence: 99%

Total antioxidant status and oxidative DNA damage in a South Indian population of essential hypertensives

et al. 2010

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To investigate the total antioxidant status (TAS) and the extent of oxidative DNA damage in total lymphocytes and their relation with essential hypertension. A total of 130 South Indian subjects aged 30-65 were recruited for the study. Of these hypertensive subjects investigated, 30 were newly diagnosed and were not on any antihypertensive drugs, but had systolic blood pressure (BP) ranging between 140 and 160 mm Hg and diastolic BP between 95 and 100 mm Hg; 50 hypertensive patients who were already on drug therapy for 1 year and 50 were normotensive controls with BP p120/80 mm Hg. DNA damage was significantly increased in hypertensive patients (both newly diagnosed and who were already on drug therapy) compared with control group. The major increase in DNA damage was observed in newly diagnosed hypertensive patients compared with hypertensive patients who were already on drug therapy. There was a significant decrease in plasma TAS value in essential hypertensive groups as compared to normotensive controls. Lymphocyte DNA damage was independently correlated with only TAS. Lymphocyte DNA damage was increased in hypertensive patients. The major increase in lymphocyte DNA damage was observed in newly diagnosed hypertensive patients compared with hypertensive patients who already on drug therapy. Decreased TAS levels, which reflect to increased oxidative stress, may be the reason of increased total lymphocyte DNA damage in South Indian hypertensive patients.

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Section: Hundred Cells Were Scored and The Number Of Viable Cells (Shmentioning

confidence: 99%

Total antioxidant status and oxidative DNA damage in a South Indian population of essential hypertensives

et al. 2010

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“…Kumaravel and Jha (2006) recommended that, for scientific purposes, both OTM and % Tail DNA could be used. Based on these findings, the Fourth IWGT also recommended the use of % Tail DNA for regulatory studies (Burlinson et al 2007). The Japanese Centre for the Validation of Alternative Methods (JaCVAM) are also recommending % Tail DNA for their inter-laboratory Comet Assay trials.…”

Section: Standardisation Of Comet Assay Measurementsmentioning

confidence: 99%

“…The main advantages of the Comet Assay include: (a) the collection of data at the level of the individual cell, allowing more robust statistical analyses, (b) the need for a small number of cells per sample (<10,000), (c) sensitivity for detecting DNA damage and (d) use of any eukaryote single cell population both in vitro and in vivo, including cells obtained from exposed human populations and aquatic organisms for eco-genotoxicological studies and environmental monitoring (Collins et al 1997a;Dixon et al 2002;Lee and Steinert 2003;Jha 2004). The importance of this assay has also been realised in regulatory genotoxicological studies (Tice et al 2000;Hartmann et al 2003;Burlinson et al 2007), and there is a move to replace some traditional assays (e.g. liver unscheduled DNA synthesis assay) in regulatory genotoxicological studies with in vivo Comet assay.…”

Section: Introductionmentioning

confidence: 99%

Comet Assay measurements: a perspective

Kumaravel¹,

Vilhar

Faux³

et al. 2007

Cell Biol Toxicol

301

155

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“…For these studies, we evaluated the levels of single-strand breaks and alkali sensitive sites in juvenile and aged cells using the Comet assay. The average olive tail moment and percent tail DNA are two different ways to analyze the amount of DNA damage from Comet data [58]. The averages for both olive tail moment (Fig.…”

Section: Effect Of Age On Hr In Cultured Primary Fibroblastsmentioning

confidence: 99%

Tissue-specific differences in the accumulation of sequence rearrangements with age

et al. 2008

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Mitotic homologous recombination (HR) is a critical pathway for the accurate repair of DNA double strand breaks (DSBs) and broken replication forks. While generally error-free, HR can occur between misaligned sequences, resulting in deleterious sequence rearrangements that can contribute to cancer and aging. To learn more about the extent to which HR occurs in different tissues during the aging process, we used Fluorescent Yellow Direct Repeat (FYDR) mice in which an HR event in a transgene yields a fluorescent phenotype. Here, we show tissue-specific differences in the accumulation of recombinant cells with age. Unlike pancreas, which shows a dramatic 23-fold increase in recombinant cell frequency with age, skin shows no increase in vivo. In vitro studies indicate that juvenile and aged primary fibroblasts are similarly able to undergo HR in response to endogenous and exogenous DNA damage. Therefore, the lack of recombinant cell accumulation in the skin is most likely not due to an inability to undergo de novo HR events. We propose that tissue-specific differences in the accumulation of recombinant cells with age result from differences in the ability of recombinant cells to persist and clonally expand within tissues.

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Fourth International Workgroup on Genotoxicity testing: Results of the in vivo Comet assay workgroup

Cited by 472 publications

References 24 publications

Total antioxidant status and oxidative DNA damage in a South Indian population of essential hypertensives

Total antioxidant status and oxidative DNA damage in a South Indian population of essential hypertensives

Comet Assay measurements: a perspective

Tissue-specific differences in the accumulation of sequence rearrangements with age

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