Fourth-Generation Enzyme-Linked Immunosorbent Assay for the Simultaneous Detection of Human Immunodeficiency Virus Antigen and Antibody

Abstract

The VIDAS HIV DUO Ultra demonstrated 100% sensitivity and 99.5% specificity overall, with a 99.7% specificity in low-risk individuals. The analytical sensitivity, as assessed by seroconversion panels and p24 antigen in samples, was equivalent to the sensitivity of the reference assays used to characterize these panels. The VIDAS HIV DUO Ultra is accurate, offers potential advantages over conventional HIV testing for time and cost savings, has walk-away capability, and correctly identifies both early and established HIV infections.

Efecto del extracto acuoso, ácido y alcohólico de las hojas secas de Erythroxylum coca var coca (coca) en Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum canis y Candida albicans in vitro

“…Al comparar dos metodologías de tamizaje de HIV (HIV Ag/Ab combo y anticuerpos contra HIV) no se encontraron diferencias significativas (p=0.776). El HIV Ag/Ac está diseñado para detectar antígeno p24 así como anticuerpos contra HIV-1, HIV-1 grupo O y HIV-2 (28) . Diversas evaluaciones entre estos métodos demuestran ventas y desventajas símiles, siendo importante su valoración concernientes a interferentes, rendimiento diagnóstico y costo-efectividad (29,30,31) .…”

Epidemiology of Human Immunodeficiency Virus in Prospective Blood Donors and Testing Algorithm Assessment with Real-Time PCR for Optimal Blood Safety Practice

“…On the other hand, if it were negative, it confirmed negative results. At the NBTS, HIV Ag-Ab detection was tested for all specimens using commercially available Genscreen Monolisa™ HIV Ag-Ab ULTRA ELISA kits manufactured by BIORAD (3,bd Raymond Poincare, 92430 Marnes-la-CoquetteFrance), according to the manufacturer's instructions [35][36][37]. …”

Detection of an Acute HIV-1 Infection Case in the Last Day of Diagnostic Window through a Combination of Two Fourth-Generation Screening Assays on a Brazilian Public Healthy Institution: We Close the Window

“…An HIV infection can potentially be detected with 4th generation HIV assays within the first 2weeks after exposure [10,13,14]. However, in addition to the first diagnostic window, a so-called second diagnostic window has been reported; this second diagnostic window may occur when 4 th generation HIV assays are used.…”

“…This is because HIV core protein appears transiently in the blood and has been used as a marker of antigenemia prior to a detectable humoral immune response directed against HIV [9]. Most people develop detectable antibody within 3-4 weeks after infection using third generation assays [10]. However, antibody may not be present in quantities greater than the minimum concentration detectable by some assays in the early stages of infection.…”

“…Detection of viral p24 protein in blood is usually earlier than detection of antibody and remains detectable for 1-2 weeks and then disappears or falls to low levels until the onset of clinical illness [6,7]. Studies have shown that detection of HIV antigen prior to the development of detectable immune response can result in a reduction of the sero-conversion window by approximately 9 days [10][11][12].…”