Fourier transform infrared spectroscopic approach to the study of the secondary cell wall development in cotton fiber

Abstract

Cotton fiber maturity is a major yield component and an important fiber quality trait that is directly linked to the quantity of cellulose deposited during the secondary cell wall (SCW) biogenesis. Cotton fiber development consists of five major overlapping stages: differentiation, initiation, polar elongation, secondary cell wall development, and maturation. The transition period between 16 and 21 dpa (days post anthesis) is regarded to represent a major developmental stage between the primary cell wall and the SCW. Fourier Transform Infrared spectroscopy was used to investigate the structural changes that occur during the different developmental stages. The IR spectra of fibers harvested at different stages of development (10, 14, 17, 18, 19, 20, 21, 24, 27, 30, 36, 46, and 56 dpa) show the presence of vibrations located at 1,733 cm -1 (C=O stretching originating from esters or amides) and 1,534 cm -1 (NH 2 deformation corresponding to proteins or amino acids). The results converge towards the conclusion that the transition phase between the primary cell wall and the secondary cell wall occurs between 17 and 18 dpa in fibers from TX19 cultivar, while this transition occurs between 21 and 24 dpa in fibers from TX55 cultivar.

Principal Component Analysis in the Development of Optical and Imaging Spectroscopic Inspections for Agricultural / Food Safety and Quality

“…Although the PC1 scores were not linear with DPAs, they identified two groups of spectra with negative PC1 scores for shorter DPAs and positive PC1 scores for longer DPAs, and further concluded the transition phase at 17 and 21 DPAs for the respective cotton varieties. The PCA results were consistent between two sampling approaches, visual inspection (Liu et al, 2011a) and differing DPAs (Abidi et al, 2010).…”

“…As a traditional practice, the cotton bolls were taken at various days post-anthesis (DPAs) to unravel a number of interests in structure, maturity, and physical properties among developing fibers. Abidi et al (2010) have employed PCA to analyze the FT-IR/ATR spectra of fibers as a function of developmental DPAs, and observed that the PC1 scores, in general, increased with DPAs for two cotton varieties. Although the PC1 scores were not linear with DPAs, they identified two groups of spectra with negative PC1 scores for shorter DPAs and positive PC1 scores for longer DPAs, and further concluded the transition phase at 17 and 21 DPAs for the respective cotton varieties.…”

Analysis of the physical properties of developing cotton fibres

“…Flowers were tagged as soon as they appeared, and harvested at the relevant dpa or days after flowering. Fibres from bolls at different developmental stages including green bolls that were 17,20,22,24,26,35,40,45,50, and 55 dpa and one boll that was matured and opened on the plant at 60 dpa were included. Bolls from 12 different plants were studied for all assays.…”

“…Abidi et al [22] studied structural changes of developing cotton fibres using FT-IR; differences in structural evolution was reported for two cotton cultivars (Gossypium hirsutum L. cv. TX19 and TX55) where transition between primary and secondary cell wall occurred between 17 and 18 dpa for TX19 cultivar fibres, and between 21 and 24 dpa for fibres from TX55 cultivar.…”

Preliminary Study of Linear Density, Tenacity, and Crystallinity of Cotton Fibers

“…Compositional and structural differences between these fibers, as well as their physical properties and end-use qualities, have been investigated considerably over the years by diversified and comprehensive techniques. Such methods include wet chemistry, microscopy, X-ray diffractometry (XRD), molecular spectroscopy and well-defined fiber testing in the cotton industry [1][2][3][4][5][6][7][8][9][10][11]. As an example, attenuated total reflection (ATR) sampling device based Fourier transform infrared (ATR-FTIR) spectroscopy has evolved as an important and successful analytical tool to investigate the plant fiber growth-induced changes in compositions and structures.…”