Fourier Transform Infrared Attenuated Total Reflection Analysis of Human Hair: Comparison of Hair from Breast Cancer Patients with Hair from Healthy Subjects

Lyman, Donald J.; Murray-Wijelath, Jacqueline

doi:10.1366/0003702052940440

Cited by 43 publications

(52 citation statements)

References 23 publications

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“…There are several potential mechanisms which could be examined, one of which is the incorporation of extraneous lipid material into the fiber as a result of the presence of a tumor. 24,26 Another mechanism may be the effect of secreted cytokines and growth factors as a result of the disease which may affect epithelial cell structures such as hair follicles. In support of this, a recent study using a highly sensitive detection system showed that serum from patients with breast cancer had a significant increase in TNFalpha when compared to age-matched healthy controls.…”

Section: Resultsmentioning

confidence: 99%

“…24 When the FTIR-ATR spectra of hairs from subjects with cancer were compared with the spectra of hairs from non-cancer subjects, differences in the amide I region [1, were observed. Interpretation of the spectra of these regions led the researchers to conclude that there was a modification of the hair fiber growth as a result of the presence of a developing cancer.…”

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confidence: 99%

“…The X-ray diffraction patterns of the hairs from women assumed to be negative for breast cancer fell into 3 categories: those that had no ring in the area of interest (17 out of 24), those that displayed a pattern consistent with disorder (4 out of 24), and those that displayed a ring in the region of interest (3 out of 24). Those 3 samples were classified as false positives, although without follow-up, it is not possible to confirm if they are true false positives.…”

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confidence: 99%

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Diagnosis of breast cancer by X‐ray diffraction of hair

Corino¹,

French²

2007

Intl Journal of Cancer

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This article presents the results of analyses of hair fibers from women with breast cancer using synchrotron-derived X-ray diffraction. These diffraction patterns contained a new feature superimposed on the normal diffraction pattern of a-keratin. The feature appeared as a ring with a molecular spacing determined to be 4.76 6 0.07 nm. This feature was not present in the diffraction patterns of hair from women without breast cancer as assessed by other routine clinical diagnostic techniques. Furthermore, different hairs from the same subject analysed on two different synchrotron beamlines give remarkably consistent diffraction patterns. Previous studies by other investigators have suggested that analysis of X-ray diffraction patterns of hair can reveal the presence of breast cancer in clinical and preclinical trials. This finding, however, has not been independently confirmed. The methodologies of sample handling, sample exposure and image analysis are known to be vital. We discuss some of these issues and provide a detailed description of the methodology employed for the sample handling and image analysis and new methodologies developed from this work. We conclude that X-ray diffraction of hair has the potential to provide a non-invasive test for the presence of breast cancer. ' 2007 Wiley-Liss, Inc.Key words: X-ray diffraction; hair; breast cancer; synchrotron In 1999 James et al. reported differences in the X-ray diffraction patterns of hair from individuals with breast cancer compared to healthy subjects.1 The X-ray diffraction patterns of hair from cancer patients contained a ring of comparatively low intensity, which was superimposed on the normal a-keratin pattern obtained from healthy control subjects and which corresponded to a molecular spacing of d 5 4.44 nm. This ring was reportedly observed in all samples of scalp and/or pubic hair taken from women diagnosed with breast cancer, as well as from subjects ''not yet diagnosed with breast cancer but suspected of being at risk''. In other words, a number of false positives were identified. Subsequent papers by James and coworkers reported X-ray diffraction analysis results of blinded human samples, which were consistent with the initial publication.2,3 The later paper reported on the results of 503 blinded hair sample analyses and demonstrated a sensitivity of 100% (no false negatives) and a specificity of 86% (14% false positives by comparison to mammography) for breast cancer.However, the finding remained highly controversial as several groups independent of James attempted to replicate the original findings and all were unsuccessful. [4][5][6][7][8][9][10][11] The technical explanations for these replication failures [12][13][14][15][16] provide insight into the experimental challenges. Myer et al., 9 whose diffraction data was not of sufficient quality to demonstrate a correlation between an altered X-ray diffraction pattern and the presence of breast cancer, sent the 27 samples they had examined to James who subsequently performed X-ray diffraction a...

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Section: Resultsmentioning

confidence: 99%

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Diagnosis of breast cancer by X‐ray diffraction of hair

Corino¹,

French²

2007

Intl Journal of Cancer

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“…Recently, Kakoulli et al reported the distribution of arsenic in ancient human hair by using synchrotron radiation [8]. Furthermore, in recent years, hair has been a widely accepted biomonitor in medical science; e.g., hair from cancer patients has been compared with hair from healthy individuals in attempts to identify possible applications in cancer research and diagnosis [9]- [11].…”

Section: Introductionmentioning

confidence: 99%

FT-IR Microspectroscopic Imaging of Cross-Sectioned Human Hair during a Bleaching Process

Ryu¹,

Jang²,

Yu³

et al. 2016

JCDSA

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We investigated bleached human hair by FT-IR microspectroscopy and chemical imaging. The cross sectioned hair is approximately 90 μm in diameter, showed cuticle and cortex in chemical imaging. Differential amide I/II absorbance ratio and broadening amide I band between in the cortex and cuticle were confirmed in FT-IR microspectroscopy and chemical imaging. The cystine monoxide band from the products of disulfide oxidation of the amino acid cystine is associated with hair damaging during bleaching process. With increase bleaching time, the band for cystine monoxide shows more intense and larger area in chemical image. The spatially chemical change was investigated in detail by FT-IR microspectroscopy and chemical imaging during the bleached process.

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“…Similarly, examination of body fluids or other material for microbial infections without requiring long culture time can be rapidly done if detection systems are developed where the type of changes associated with different kinds of microbes are clearly defined and established (Bombalska et al 2011, Maquelin et al 2003. The ability of FTIR to detect biochemical changes in organs other than the one affected to possibly use these as indicator of the health status of patients has been another growing area of interest, mainly because these use materials like hairs and nails that are dispensable or blood samples that are easily obtained compared to biopsies (Lyman et al 2005, Khanmohammadi et al 2007. This type of FTIR based diagnosis would help decrease the cost through early intervention.…”

Section: Recent Trends and Future Perspectivesmentioning

confidence: 99%