Fourier-Domain Optical Coherence Tomography Imaging in Corneal Epithelial Basement Membrane Dystrophy: A Structural Analysis

Sanharawi, Mohamed El; Sandali, Otman; Basli, Elena; Bouheraoua, Nacim; Améline, B; Goemaere, Isabelle; Georgeon, Cristina; Hamiche, Taous; Laroche, L.

doi:10.1016/j.ajo.2015.01.003

Cited by 24 publications

(15 citation statements)

References 18 publications

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“…Regarding CD corneal morphology analysis, our current SS OCT study complements previous findings demonstrated based on TD, SD, and SS OCT [ 19 – 23 , 32 , 33 ].…”

Section: Discussionsupporting

confidence: 84%

“…Currently, there are no data on anterior eye segment measurements with SS OCT in various corneal dystrophies. Previous papers on OCT imaging focused on describing corneal morphology features in different CDs [ 19 – 23 ]. The SS OCT was proved useful in planning of the phototherapeutic keratectomy to treat granular corneal dystrophy by determining the size, depth, and location of deposits based on the case report study [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Study of Anterior Eye Segment Measurements with Spectral Swept-Source and Time-Domain Optical Coherence Tomography in Eyes with Corneal Dystrophies

Nowińska

Teper

Janiszewska

et al. 2015

BioMed Research International

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Purpose. To compare anterior eye segment measurements and morphology obtained with two optical coherence tomography systems (TD OCT, SS OCT) in eyes with corneal dystrophies (CDs). Methods. Fifty healthy volunteers (50 eyes) and 54 patients (96 eyes) diagnosed with CD (epithelial basement membrane dystrophy, EBMD = 12 eyes; Thiel-Behnke CD = 6 eyes; lattice CD TGFBI type = 15 eyes; granular CD type 1 = 7 eyes, granular CD type 2 = 2 eyes; macular CD = 23 eyes; and Fuchs endothelial CD = 31 eyes) were recruited for the study. Automated and manual central corneal thickness (aCCT, mCCT), anterior chamber depth (ACD), and nasal and temporal trabecular iris angle (nTIA, tTIA) were measured and compared with Bland-Altman plots. Results. Good agreement between the TD and SS OCT measurements was demonstrated for mCCT and aCCT in normal individuals and for mCCT in the CDs group. The ACD, nTIA, and tTIA measurements differed significantly in both groups. TBCD, LCD, and FECD caused increased CCT. MCD caused significant corneal thinning. FECD affected all analyzed parameters. Conclusions. Better agreement between SS OCT and TD OCT measurements was demonstrated in normal individuals compared to the CDs group. OCT provides comprehensive corneal deposits analysis and demonstrates the association of CD with CCT, ACD, and TIA measurements.

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“…Regarding CD corneal morphology analysis, our current SS OCT study complements previous findings demonstrated based on TD, SD, and SS OCT [ 19 – 23 , 32 , 33 ].…”

Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Comparative Study of Anterior Eye Segment Measurements with Spectral Swept-Source and Time-Domain Optical Coherence Tomography in Eyes with Corneal Dystrophies

Nowińska

Teper

Janiszewska

et al. 2015

BioMed Research International

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“…The corneal epithelium was variably thickened, consistent with epithelial hyperplasia, and the foci of hyper-intense signal in the anterior stroma likely represented the increased cellularity noted on histopathology. OCT is routinely used in physician ophthalmology and allows for non-contact, non-invasive imaging that assists in the diagnosis and management of several corneal diseases, including ocular surface squamous neoplasia, corneal epithelial basement membrane dystrophy, keratoconus and bullous keratopathy (Chan et al 2011, Bouheraoua et al 2014, Thomas et al 2014, El Sanharawi et al 2015). People with corneal scarring associated with an irregular Bowman’s membrane undergo OCT imaging prior to phototherapeutic keratectomy (PTK) to measure the depth of their corneal scars and allow for surgical planning.…”

Section: Discussionmentioning

confidence: 99%

Development of a novel in vivo corneal fibrosis model in the dog

Gronkiewicz

Giuliano

Kuroki

et al. 2016

Experimental Eye Research

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The aim of this study was to develop a novel in vivo corneal model of fibrosis in dogs utilizing alkali burn and determine the ability of suberanilohydroxamic acid (SAHA) to inhibit corneal fibrosis using this large animal model. To accomplish this, we used seven research Beagle dogs. An axial corneal alkali burn in dogs was created using 1 N NaOH topically. Six dogs were randomly and equally assigned into 2 groups: A) vehicle (DMSO, 2 μL/mL); B) anti-fibrotic treatment (50 μM SAHA). The degree of corneal opacity, ocular health, and anti-fibrotic effects of SAHA were determined utilizing the Fantes grading scale, modified McDonald-Shadduck (mMS) scoring system, optical coherence tomography (OCT), corneal histopathology, immunohistochemistry (IHC), and transmission electron microscopy (TEM). The used alkali burn dose to produce corneal fibrosis was well tolerated as no significant difference in mMS scores between control and treatment groups (p=0.89) were detected. The corneas of alkali burned dogs showed significantly greater levels of α-smooth muscle actin, the fibrotic marker, than the controls (p=0.018). Total corneal thickness of all dogs post-burn was significantly greater than baseline OCT images irrespective of treatment (p=0.004); TEM showed that alkali burned corneas had significantly greater minimum and maximum interfibrillar distances than the controls (p=0.026, p=0.018). The tested topical corneal alkali burn dose generated significant opacity and fibrosis in dog corneas without damaging the limbus as evidenced by histopathology, IHC, TEM, and OCT findings, and represents a viable large animal corneal fibrosis in vivo model. Additional in vivo SAHA dosing studies with larger sample size are warranted.

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“…One patient presented an irregular epithelial basement membrane expanding into the epithelium which is a common finding in corneal epithelial basement membrane dystrophy [19]. Association of SCD with the latter dystrophy/degeneration may be the consequence of the high prevalence of the latter corneal disorder.…”

Section: Discussionmentioning

confidence: 94%

Multimodal Imaging Features of Schnyder Corneal Dystrophy

Ghazal¹,

Georgeon²,

Grieve

et al. 2020

Journal of Ophthalmology

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Objective. To describe the multimodal imaging of Schnyder corneal dystrophy. Methods. Seven eyes of seven patients (5 female and 2 male patients) aged 52 to 92 years were included in this prospective observational study. Diagnosis of SCD was confirmed by histology after keratoplasty. In vivo multimodal imaging consisted of spectral domain-optical coherence tomography with cross sections, en face scans, corneal pachymetry, and epithelial mapping, and in vivo confocal microscopy was recorded. Ex vivo full-field optical coherence tomography scans of two corneal buttons were analyzed. The seven corneal buttons obtained during penetrating or deep anterior lamellar keratoplasty were processed for light microscopy. Results. Slit-lamp examination showed central stromal opacities, arcus lipoides, and midperipheral haze. Corneal crystals were found in 2 out of 7 eyes. SD-OCT cross sections and en face scans showed diffuse hyperreflectivity of the anterior, mid, and posterior stroma with a maximum in the anterior stroma, hyporeflective stromal striae, and epithelial hyperreflectivity. Central corneal thickness ranged from 507 to 635 μm. IVCM revealed hyperreflective deposits in the epithelium and throughout the stroma, thin subepithelial nerves, and needle-shaped and rectangular crystals. Keratocyte nuclei were rare or undetectable. FF-OCT scans confirmed the presence of small round and needle-shaped hyperreflective deposits in the epithelium and stroma. Histology revealed vacuolization of the basal epithelial cells and empty interlamellar stromal vacuoles. Conclusion. High-resolution multimodal imaging demonstrates the characteristic features of SCD which involve both the corneal epithelium and stroma, and it provides diagnosis confirmation even in eyes with no visible corneal crystals at slit-lamp examination.

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Fourier-Domain Optical Coherence Tomography Imaging in Corneal Epithelial Basement Membrane Dystrophy: A Structural Analysis

Cited by 24 publications

References 18 publications

Comparative Study of Anterior Eye Segment Measurements with Spectral Swept-Source and Time-Domain Optical Coherence Tomography in Eyes with Corneal Dystrophies

Comparative Study of Anterior Eye Segment Measurements with Spectral Swept-Source and Time-Domain Optical Coherence Tomography in Eyes with Corneal Dystrophies

Development of a novel in vivo corneal fibrosis model in the dog

Multimodal Imaging Features of Schnyder Corneal Dystrophy

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