Fourier-domain digital holographic optical coherence imaging of living tissue

Abstract

Digital holographic optical coherence imaging is a full-frame coherence-gated imaging approach that uses a CCD camera to record and reconstruct digital holograms from living tissue. Recording digital holograms at the optical Fourier plane has advantages for diffuse targets compared with Fresnel off-axis digital holography. A digital hologram captured at the Fourier plane requires only a 2D fast Fourier transform for numerical reconstruction. We have applied this technique for the depth-resolved imaging of rat osteogenic tumor multicellular spheroids and acquired cross-section images of the anterior segment and the retinal region of a mouse eye. A penetration depth of 1.4 mm for the tumor spheroids was achieved.

Digital Holography and Cell Studies

“…Taking it one step further, DHM can be used for tomographic measurements (Massatsch et al, 2005) that enable analysis of sub-cellular motion from deep within living tissues (Jeong et al, 2007a), along with valuable 3-D-reconstruction of the object (volumetric visualization) (Jeong et al, 2007b). Sun and colleagues showed that they could follow the movement of blood cells in vivo in a tadpole blood vessel (Sun et al, 2008).…”

Tissue Dynamics Spectroscopy for Three-Dimensional Tissue-Based Drug Screening

“…75,76 This soon progressed to MCI that spatially resolved the autocorrelation functions and used overall motion as a novel contrast agent. 24,34 Now it has evolved to TDS 77 that resolves overall motion into at least three broad frequency ranges with a tentative assignment of microscopic intracellular motion mechanisms.…”

“…Most of the zero-order images are removed after this zero-order suppression, the data are more stable, and it is less time consuming than acquiring a non-zero-path-matched image for zero-order subtraction. 76 The structure reconstructed in 3D is not static in living tissue, but is highly dynamic, reflecting the multitude of motions that occur on the subcellular scale. These motions include organelle transport by molecular motors, active motions of the membrane, membrane shape changes and restructuring of cellecell and celleECM contacts, mitosis and cytokinesis, and necrosis and apoptosis.…”

Biodynamic imaging of live porcine oocytes, zygotes and blastocysts for viability assessment in assisted reproductive technologies

“…It is well suited for the evaluation of oocyte and embryo quality because it captures the functional dynamics of the cells and tissues without exogenous dyes. Biodynamic imaging uses lowcoherence Fourier-domain digital holography [22] to collect back-scattered light from living tissue up to 1 mm in thickness. Tissue dynamics spectroscopy [23,24] separates the motion into frequency bands related to Doppler frequencies of the scattered light.…”