Four weeks of light-intensity exercise enhances empathic behavior in mice: The possible involvement of BDNF

Shima, Takeru; Kawabata‐Iwakawa, Reika; Onishi, Hayate; Jesmin, Subrina; Yoshikawa, Tomonori

doi:10.1016/j.brainres.2022.147920

Cited by 4 publications

(2 citation statements)

References 43 publications

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“…Mice in the exercise group underwent a running habituation phase on a forced exercise wheel bed for 30 min/day, five days/week (a total of five sessions over six days) at speeds ranging from 3.0 to 7.0 m/min for C57BL/6 mice and from 3.0 to 5.0 m/min for ob/ob mice, spanning one week. Subsequently, they engaged in light-intensity exercise sessions (C57BL/6 mice, 7.0 m/min; ob/ob mice, 5.0 m/min) on the same equipment for 30 min/day, five days/week over three weeks [11,43,44]. The exercise intensity for each strain was established based on their ventilatory threshold [44].…”

Section: Methodsmentioning

confidence: 99%

Muscle-derived miR-200a-3p through light-intensity exercise may contribute to improve memory dysfunction in type 2 diabetic mice

Shima,

Onishi,

Terashima

2024

Preprint

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Background: Memory dysfunction associated with type 2 diabetes mellitus (T2DM) poses a considerable threat to overall well-being. Engaging in light-intensity exercise has been shown to exert favorable effects on hippocampal function and molecular profiles, including Mct2 mRNA and miR-200a-3p. Nonetheless, a comprehensive understanding of the mechanisms underlying the positive impact of light-intensity exercise remains elusive. Here, we assessed the influence of exosomal miR-200a-3p secretion from gastrocnemius muscles in T2DM mice undergoing light-intensity exercise intervention, focusing on its potential to ameliorate memory dysfunction. Basic procedures: We initially assessed the effects of light-intensity exercise (7.0 m/min for healthy mice, 5.0 m/min for ob/ob mice, 30 min/day, five days/week, over a four-week period) on memory function, hippocampal mRNA associated with memory function, and the secretion of exosomal miR-200a-3p from their gastrocnemius muscle. Subsequently, the impact of a daily intraperitoneal injection of the miR-200a-3p mimic over a four-week duration was investigated, focusing on its influence on hippocampal dysregulation in ob/ob mice. Main findings: The light-intensity exercise intervention increased gastrocnemius muscle-derived and plasma exosomal miR-200a-3p levels in ob/ob mice, concomitant with improved memory dysfunction. Intriguingly, the daily intraperitoneal injection of mmu-miR-200a-3p mimic also demonstrated an ameliorative effect on memory function in ob/ob mice. Notably, both the exercise intervention and miR-200a-3p mimic treatment induced downregulation in hippocampal Keap1 mRNA and upregulation in mRNA of Hsp90aa1 and Mct2 in ob/ob mice. Principal conclusions: The current results imply that the augmentation of exosomal miR-200a-3p derived from the gastrocnemius muscle contributes to the amelioration of memory dysfunction in T2DM undergoing light-intensity exercise. Additionally, it is proposed that miR-200a-3p emulates the effects of light-intensity exercise, suggesting a potential therapeutic pathway for addressing hippocampal complications in the context of T2DM.

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Section: Methodsmentioning

confidence: 99%

Muscle-derived miR-200a-3p through light-intensity exercise may contribute to improve memory dysfunction in type 2 diabetic mice

Shima,

Onishi,

Terashima

2024

Preprint

Self Cite

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“…MiRNAs are known to function by binding to complementary segments of messenger RNAs (mRNAs), subsequently leading to the degradation or inhibition of mRNA translation [18]. Their roles in brain development and functions have been documented [19][20][21], and changes in miRNA profiles are recognized as important regulators and biomarkers for dietary effects [22][23][24]. Moreover, variations in the nutritional composition of diets have been shown to influence miRNA profiles in various organs and circulation [25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Feeding a low-carbohydrate and high-protein diet diminishes working memory in healthy mice: possible involvement of miR-539-3p/Lrp6/Igf1raxis in the hippocampus

Shima,

Onishi,

Terashima

2024

Preprint

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A low-carbohydrate and high-protein (LC-HP) diet demonstrates favorable impacts on metabolic parameters, albeit it leads to a decline in hippocampal function among healthy mice. The reduction in working memory induced by LC-HP diets is attributed to the decreased expression of hippocampal IGF-1 receptor (IGF-1R). However, the precise mechanisms underlying this phenomenon remain unexplored. Here, we investigated that by analyzing alterations in hippocampal miRNA profiles. C57BL/6 mice were divided into the LC-HP diet-fed group (25.1% carbohydrate, 57.2% protein, and 17.7% fat as percentages of calories) and the control diet-fed group (58.9% carbohydrate, 24.0% protein, and 17.1% fat as percentages of calories). After four weeks, all mice underwent the Y-maze test, followed by analyses of mRNA and miRNA expressions in the hippocampus. Our investigation revealed that feeding the LC-HP diet suppressed working memory function and hippocampal Igf1r mRNA levels in mice. Sequencing of miRNA demonstrated 17 upregulated and 27 downregulated miRNAs in the hippocampus of LC-HP diet-fed mice. Notably, upregulation of miR-539-3p, predicted to modulate Igf1r gene expression, was observed. Consequently, we found decreased hippocampal mRNA levels of low-density lipoprotein receptor-related protein 6 (Lrp6), a gene modulated by miR-539-3p, in mice fed the LC-HP diet. Furthermore, a significant positive correlation was observed between Lrp6 and Igf1r mRNA levels in the hippocampus. These findings suggest that LC-HP diets may suppress hippocampal function via the miR-539-3p/Lrp6/Igf1r axis, offering a potential target for nutritional strategies to preserve hippocampal health.

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Circulating miRNAs as potential biomarkers for light intensity exercise-induced enhancements in empathy

Shima,

Kawabata-Iwakawa,

Onishi

et al. 2023

Sport Sci Health

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Four weeks of light-intensity exercise enhances empathic behavior in mice: The possible involvement of BDNF

Cited by 4 publications

References 43 publications

Muscle-derived miR-200a-3p through light-intensity exercise may contribute to improve memory dysfunction in type 2 diabetic mice

Muscle-derived miR-200a-3p through light-intensity exercise may contribute to improve memory dysfunction in type 2 diabetic mice

Feeding a low-carbohydrate and high-protein diet diminishes working memory in healthy mice: possible involvement of miR-539-3p/Lrp6/Igf1raxis in the hippocampus

Circulating miRNAs as potential biomarkers for light intensity exercise-induced enhancements in empathy

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