Four targeted genes for predicting the prognosis of colorectal cancer: A bioinformatics analysis case

Abstract

The molecular mechanisms underlying the development and progression of colorectal cancer (CRC) have not been clarified. The purpose of the present study was to identify key genes that may serve as novel therapeutic targets or prognostic predictors in patients with CRC using bioinformatics analysis. Four gene expression datasets were downloaded from the Gene Expression Omnibus database, which revealed 19 upregulated and 34 downregulated differentially expressed genes (DEGs). The downregulated DEGs were significantly enriched in eight pathways according to Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. A protein-protein interaction network was constructed with 52 DEGs and 458 edges. Ten key genes were identified according to the degree value, betweenness centrality and closeness centrality. Survival analysis revealed that low expression of four of the ten genes, carcinoembryonic antigen related cell adhesion molecule 7 (CEACAM7), solute carrier family 4 member 4 (SLC4A4), glucagon (GCG) and chloride channel accessory 1 (CLCA1) genes, were associated with unfavorable prognosis in CRC. Furthermore, gene set enrichment analysis revealed that two pathways were significantly enriched in the CEACAM7 low-expression group. Thus, CEACAM7, SLC4A4, GCG and CLCA1 may be prognostic markers or therapeutic targets of CRC. Low CEACAM7 expression may be associated with the activation of glycosaminoglycan biosynthesis-chondroitin sulfate and extracellular matrix receptor interaction pathways and may affect the prognosis of CRC.

SLC1A1, SLC16A9, and CNTN3 Are Potential Biomarkers for the Occurrence of Colorectal Cancer

“…For example, it has been found that CXCL3 [28] and CXCL8 [40] participate in the pathogenesis of UC and can be used as therapeutic targets for UC. For CRA, a study by Mclean et al showed that the inflammatory cytokine genes CXCL1, CXCL2, CXCL3, CCL20, IL8 (CXCL8), CCL23, CCL19, CCL21, and CCL5are BioMed Research International 4) and CEACAM7 (carcinoembryonic antigen-related cell adhesion molecule 7) have been found to be associated with an unfavourable prognosis in CRC [41]. SLC4A4 was also found to be a differentially expressed gene common to UC and CRC [42].…”

Identification of DEGs and transcription factors involved in H. pylori-associated inflammation and their relevance with gastric cancer

“…APOA4 was reported to be closely related to urinary bladder cancer (Soukup et al, 2019). CYP3A4 is currently indicated for the treatment of ovarian and breast cancer (Fiszer-Maliszewska et al, 2018;Liu et al, 2019). Bian et al (2019) found that GCG affected the development and progression of colon cancer.…”

“…CYP3A4 is currently indicated for the treatment of ovarian and breast cancer (Fiszer-Maliszewska et al, 2018;Liu et al, 2019). Bian et al (2019) found that GCG affected the development and progression of colon cancer. Li et al (2019) demonstrated that XPNPEP2 was associated with lymph node metastasis in prostate cancer patients.…”

Prognostic and Clinicopathological Insights of Phosphodiesterase 9A Gene As Novel Biomarker in Human Colorectal Cancer

“…Down-regulated gene expression of this gene may be inspected in the colon and rectal cancer [79,80]. CEACAM7 plays important role in cancer pathology and may provide valuable information to predict promising prognostic markers of CRC and to unravel the molecular mechanism of CRC [73,81,82].…”