Four specific isogenes of the anthocyanin metabolic pathway are systematically co-expressed with the red colour of grape berries

Ageorges, Agnès; Fernández, Luis Moreno; Vialet, Sandrine; Merdinoglu, Didier; Terrier, Nancy; Romieu, Charles

doi:10.1016/j.plantsci.2005.09.007

Cited by 161 publications

(134 citation statements)

References 34 publications

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“…We found that the expression of GST in Shuang Hong was low from 2 WAF to veraison, and then up-regulated from veraison to maturity at which point it reached its highest level of expression. These results are consistent with others (Castellarin et al, 2007a ;Ageorges et al, 2006;Jeong et al, 2004). The GST genetic transcriptional level had a positive correlation with the content of anthocyanins from veraison to maturity (R 2 =0.9436) ( Figure 5E; Table 2).…”

Section: Gst Gene Expression During Berry Developmentsupporting

confidence: 94%

“…Glutathione S-transferase (GST) may be last component involved in anthocyanin biosynthesis (Ageorges et al, 2006;Marrs et al, 1995;Alfenito et al, 1998). Flavonoids may be conjugated with glutathione (GSH) through a reaction catalyzed by GST (Braidot et al, 2008).…”

Section: Gst Gene Expression During Berry Developmentmentioning

confidence: 99%

“…It has been suggested that anthocyanins could be transported into the vacuoles via the noncovalent activity of GSTs (Marrs et al, 1995;Alfenito et al, 1998;Mueller et al, 2000). A member of the GST gene family showing overlapping patterns of expression with anthocyanin accumulation was identified by Ageorges et al (2006). For the majority of red grape cultivars, the anthocyanins accumulate mainly in the vacuoles of the hypodermal cell layers of the berry skin after veraison (Boss et al, 1996b;Chen et al, 2006;Fung et al, 2007;Ramazzotti et al, 2008;Barcelóet al, 1994).…”

Section: Gst Gene Expression During Berry Developmentmentioning

confidence: 99%

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Expression of Structural Genes Related to Anthocyanin Biosynthesis of <i>Vitis amurensis</i>

Zhao¹,

He²,

Malcolm³

et al. 2013

mpb

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The accumulation of anthocyanins in skins is one of the main features of red grape berry ripening. This research was designed to assess the changes in anthocyanin content in grape skins of V. amurensis and to explore mRNA transcriptions of 11 structural genes (PAL, CHS3, CHI1, F3H2, F3'H, F3'5'H, DFR, LDOX, UFGT, OMT and GST) related to anthocyanin biosynthesis during grape berry development, by the use of HPLC-MS/MS and real-time Q-PCR analysis. Accumulation of anthocyanins began at veraison, continued throughout the later berry development and reached a peak at maturity. Veraison is the time when the berries turn from green to purple. Expression of PAL, CHI1, and LDOX were up-regulated from 2 to 4 weeks after flowering (WAF), down-regulated from 6 WAF to veraison, whereas DFR was up-regulated at 8 WAF, and then up-regulated from veraison to maturity. The expression of these genes at the transcriptional level had a positive correlation with the content of anthocyanins from veraison to maturity. The CHS3, F3'5'H, UFGT, GST, and OMT were down-regulated from 2 WAF to veraison, and then up-regulated from veraison to maturity. The transcriptional expressions of the 11 structural genes also showed positive correlations with the anthocyanin content from veraison to maturity. Positive correlations were also observed between OMT transcriptional level and the content of methoxyl-anthocyanins and between F3'5'H transcriptional level and the content of delphinidin anthocyanins. F3H2 and F3'H expression was up-regulated at 2 WAF. F3H2 expression was down-regulated from 4 WAF to veraison and then up-regulated again from veraison to maturity. F3'H expression was down-regulated at 4 WAF and then up-regulated again from 6 WAF to maturity. F3'H transcriptional level was correlated positively with the cyanidin anthocyanin concentration from veraison to maturity. These results indicate that the onset of anthocyanin synthesis during berry development coincides with a coordinated increase in the expression of a number of genes in the anthocyanin biosynthetic pathway.

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Section: Gst Gene Expression During Berry Developmentsupporting

confidence: 94%

Section: Gst Gene Expression During Berry Developmentmentioning

confidence: 99%

Section: Gst Gene Expression During Berry Developmentmentioning

confidence: 99%

See 1 more Smart Citation

Expression of Structural Genes Related to Anthocyanin Biosynthesis of <i>Vitis amurensis</i>

Zhao¹,

He²,

Malcolm³

et al. 2013

mpb

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“…Among these transcriptional regulators, the MYB family, which has been largely investigated in grape, constitutes the most abundant group. MYBA1 and MYBA2 are able to induce gene expression of UDP Glc flavonoid 3-Oglucosyltransferase that catalyzes one of the last steps in the anthocyanin biosynthetic pathway (Kobayashi et al, 2002;Ageorges et al, 2006;Walker et al, 2007). Moreover, the white phenotype in grapes has been related to the presence of a transposable element, Gret1, in the promoter of the MYBA1 locus (Kobayashi et al, 2004), showing the relevance of MYBA1 as a regulator of anthocyanins biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

“…For anthocyanin determination from HRs, 200 mg of ground, frozen tissues was used. Determination of anthocyanin composition was performed by HPLC as described by Ageorges et al (2006).…”

Section: Quantitative Analysis Of Anthocyaninsmentioning

confidence: 99%

ABCC1, an ATP Binding Cassette Protein from Grape Berry, Transports Anthocyanidin 3-O-Glucosides

et al. 2013

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ORCID ID: 0000-0002-9663-5371 (RN).Accumulation of anthocyanins in the exocarp of red grapevine (Vitis vinifera) cultivars is one of several events that characterize the onset of grape berry ripening (véraison). Despite our thorough understanding of anthocyanin biosynthesis and regulation, little is known about the molecular aspects of their transport. The participation of ATP binding cassette (ABC) proteins in vacuolar anthocyanin transport has long been a matter of debate. Here, we present biochemical evidence that an ABC protein, ABCC1, localizes to the tonoplast and is involved in the transport of glucosylated anthocyanidins. ABCC1 is expressed in the exocarp throughout berry development and ripening, with a significant increase at véraison (i.e., the onset of ripening). Transport experiments using microsomes isolated from ABCC1-expressing yeast cells showed that ABCC1 transports malvidin 3-Oglucoside. The transport strictly depends on the presence of GSH, which is cotransported with the anthocyanins and is sensitive to inhibitors of ABC proteins. By exposing anthocyanin-producing grapevine root cultures to buthionine sulphoximine, which reduced GSH levels, a decrease in anthocyanin concentration is observed. In conclusion, we provide evidence that ABCC1 acts as an anthocyanin transporter that depends on GSH without the formation of an anthocyanin-GSH conjugate.

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Comparative phenotypic and transcriptomic analysis of Victoria and flame seedless grape cultivars during berry ripening

et al. 2020

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Grape berry development is a highly coordinated and intricate process. Herein, we analyzed the phenotypic and transcriptomic patterns of Victoria (VT) and Flame Seedless (FS) grape varieties during berry development. Physiological analysis and transcriptomic sequencing were performed at four berry developmental phases. VT berry size was comparatively larger to the FS variety. At maturity, 80 days post-anthesis (DPA), the FS soluble solids were 61.8% higher than VT. Further, 4,889 and 2,802 differentially expressed genes were identified from VT and FS 40 DPA to 80 DPA development stages, respectively. VvSWEET15, VvHXK and MYB44 genes were up-regulated during the post-anthesis period, while bHLH14, linked to glucose metabolism, was gradually down-regulated during berry development. These genes may have significant roles in berry development, ripening and sugar accumulation.

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Four specific isogenes of the anthocyanin metabolic pathway are systematically co-expressed with the red colour of grape berries

Cited by 161 publications

References 34 publications

Expression of Structural Genes Related to Anthocyanin Biosynthesis of <i>Vitis amurensis</i>

Expression of Structural Genes Related to Anthocyanin Biosynthesis of <i>Vitis amurensis</i>

ABCC1, an ATP Binding Cassette Protein from Grape Berry, Transports Anthocyanidin 3-O-Glucosides

Comparative phenotypic and transcriptomic analysis of Victoria and flame seedless grape cultivars during berry ripening

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