Four serum microRNAs identified as diagnostic biomarkers of sepsis

Abstract

II, diagnostic study.

How might a diagnostic microRNA signature be used to speed up the diagnosis of sepsis?

“…These pathways are relevant to sepsis and PRR pathway-specific miRNA signatures and have a potential role as diagnostic biomarkers to differential sterile versus infected inflammation. In a heterogeneous cohort of 166 patients with sepsis, a reduction in miR-499-5p was associated with prediction of sepsis-related severity of illness [13]. In this study, at a cut-off point of 0.751, the area under the receiver operated curve for miR-499-5p was 0.686 (0.592-0.779) with a sensitivity and specificity of 68 and 65% respectively [13].…”

“…In a heterogeneous cohort of 166 patients with sepsis, a reduction in miR-499-5p was associated with prediction of sepsis-related severity of illness [13]. In this study, at a cut-off point of 0.751, the area under the receiver operated curve for miR-499-5p was 0.686 (0.592-0.779) with a sensitivity and specificity of 68 and 65% respectively [13]. Another study using a cohort of 50 patients with culture-positive sepsis following major abdominal surgery or trauma with postcardiac surgical patients as systemic inflammatory response syndrome controls, a reduction in miR-146a and miR-223 was observed and proposed as a potential biomarker in sepsis [21].…”

“…Thus, the chromosomal location and mechanisms of post-transcriptional regulation by miRNA provide a biological system where the role of miRNA in a particular pathway can be studied. In the context of infection-induced critical illness in humans, miRNA-based molecular signatures have been proposed as surrogate markers that could differentiate between sterile inflammation and sepsis [11], be diagnostic for sepsis [13], influence drug response in sepsis [14], be responsible for altered protein synthesis in muscle during critical illness [15] and potentially have prognostic utility [16,17].…”

CNAPS and General Medicine

“…In 166 patients with sepsis and 24 healthy controls, the expression levels of several miRNAs were determined by RT qPCR. Serum levels of miR-223 (p < 0.04) and miR-499-5p (p < 0.001) were significantly different between patients with mild sepsis and those with severe sepsis and septic shock [31]. Serum miR-499-5p, also a biomarker of acute myocardial infarction [14], had the highest AUC of 0.69 followed by serum miR-223 with an AUC of 0.61 [31].…”

The role and regulation of inhibitor of Kappa B Kinase in the impaired monocyte response.

“…Furthermore, various studies 56 have identified miRNAs that are dysregulated in patients with sepsis [104][105][106][107] .…”