Four New Species of <i>Amanita</i> in Inje County, Korea

Cho, Hae Jin; Park, Myung Soo; Lee, Hyun; Oh, Seung‐Yoon; Jang, Yeongseon; Fong, Jonathan J.; Lim, Young Woon

doi:10.5941/myco.2015.43.4.408

Cited by 16 publications

(14 citation statements)

References 20 publications

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“…(5'-TCCTCCGCTTATTGATATGC-3') as previously described (Cho et al 2015;Kim et al 2016;Lee et al 2000;Persoh et al 2009;Raja et al 2017). PCR reactions were performed in a total volume of 20 µl consists of 6 µl distilled water, 10 µl 2x PCR master mix (iNtRONbiotechnology, Korea), 1 µl of each 10 µM primer (0.5 µM) and 2 µl template DNA.…”

Section: Pcr Ampli Cation Of Internal Transcribed Spacer (Its) Regionmentioning

confidence: 99%

“…It is di cult to differentiate closely related species. However, molecular techniques such as polymerase chain reaction (PCR) for mushroom identi cation have proved to be fast, speci c, and sensitive, especially, inconsistent morphology and indiscrimination among species of mushrooms (Cho et al 2015;Kim et al 2016;Lee et al 2000;Persoh et al 2009;Raja et al 2017). The conserved region such as Internal Transcribed Spacer (ITS) of nuclear ribosomal DNA (rDNA) usually has been targeted because it is highly conserved among interspecies but variable between intraspecies (Raja et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Ascomycetes Mushroom in Para Rubber Plantation, Thailand

Surawut¹,

Nak-aim²,

Kunsook³

et al. 2021

Preprint

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Ascomycetes mushrooms are fungi that produce ascospores in asci and some with perithecia. Not only they have a role of decomposer in ecology but also produced some bioactive compound, anti-microbial activity, and cytotoxicity. This study aims to explore the diversity of ascomycetes mushroom species in para rubber plantations and to identify them by morphological and sequence analysis of the internal transcribed spacer (ITS) region. The results found ascomycetes mushroom consist of Trichoderma pezizoides (RP1, % identity 98.79, DQ835513.1), Daldinia eschscholtzii (RP2, % identity 100, MN310384.1), Cookeina sulcipes (RP3, % identity 98.44, KY094620.1), Cookeina garethjonesii (RP4, % identity 99.06, KY094622.1), Cookeina tricholoma (RP5, % identity 100, KY094619.1) and Xylaria terricola (RP6, % identity 88.42, MF577038.1). Most of the ascomycetes in this study have previously been described in Thailand except Xylaria terricola. Additionally, phylogenetic analysis of ascomycetes mushroom showed high genetic relatedness with reference strains. Therefore, the sequence similarity and phylogenetic analysis confirmed the identity of six ascomycetes mushroom species, and further study of bioactive compound from these mushrooms may be investigated for other applications.

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Section: Pcr Ampli Cation Of Internal Transcribed Spacer (Its) Regionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diversity of Ascomycetes Mushroom in Para Rubber Plantation, Thailand

Surawut¹,

Nak-aim²,

Kunsook³

et al. 2021

Preprint

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“…The genus Amanita consists of 95% of the species which are characterized by agaricoid basidiomata, colorless and hyaline, ballistosporic and smooth basidiospores, free lamellae, presence of volval remnants (Persoon 1797). A total of 540 known species of Amanita are distributed worldwide (Yang 2000, Kirk et al 2008, Menolli et al 2009, Tulloss 2009, Wartchow et al 2009, Justo et al 2010, Wartchow and Gamboa-Trujillo 2012, Cho et al 2015, Hosen et al 2015, Tang et al 2015, Wartchow and Cortez 2016, Jabeen et al 2017, Cui et al 2018, Kiran et al 2018a, b). From Pakistan, 19 species of Amanita are known to date (Ahmad et al 1997, Jabeen et al 2017, Kiran et al 2018a, b).…”

Section: Introductionmentioning

confidence: 99%

Amanita ahmadii, a new species of Amanita subgenus Amanitina section Validae from Pakistan

Jabeen

Kiran

Khan

et al. 2019

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A new species from coniferous forests in Pakistan, Amanitaahmadii, is described on the basis of morpho-anatomy and molecular data set analyses. This species is characterized by its medium-sized to large basidiomata, grayish brown to brown pileal surface and rimose pileus margin with gray to dark brown verrucose veil remnants, a cream stipe with bulbous base having grayish brown or brown longitudinal striations above the annulus, a scaly surface towards the base, globose to broadly ellipsoid and amyloid basidiospores, and the absence of clamped septa in all tissues. Molecular phylogenetic analyses based on ITS and LSU sequences confirmed its identity as a new taxon nested within subgen. Amanitinasect.Validae.

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“…Although several coding genes have been used in recent fungal phylogeny studies, the ITS region remains the most commonly used locus for fungal phylogenetics, and the accumulated DNA sequence information for the ITS region has greatly improved the accuracy of identification in the Fungi [ 14 15 16 17 ]. Previously, we showed the efficiency and accuracy of a two-step approach for fungal identification: phylogenetic analysis using ITS sequences followed by confirmation based on morphological observations [ 18 19 20 21 ].…”

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confidence: 99%

Three New Recorded Species of the Physalacriaceae on Ulleung Island, Korea

Park

Kim

et al. 2017

Mycobiology

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Most known species in the Physalacriaceae are saprotrophs that grow on decaying leaves and wood, and approximately 21 genera in the Physalacriaceae have been reported worldwide. During an ongoing survey of indigenous fungi in Korea, four specimens belonging to the Physalacriaceae were collected on Ulleung Island. These specimens were identified as three species based on morphological characteristics and molecular analysis of rDNA-internal transcribed spacer sequences. Three species in three genera were shown to be new records in Korea: Hymenopellis orientalis, Paraxerula hongoi, and Ponticulomyces orientalis. The latter two are the first records of these genera in Korea. In this study, we provide detailed morphological descriptions of these species and describe their phylogenetic position within the Physalacriaceae.

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Four New Species of Amanita in Inje County, Korea

Cited by 16 publications

References 20 publications

Diversity of Ascomycetes Mushroom in Para Rubber Plantation, Thailand

Diversity of Ascomycetes Mushroom in Para Rubber Plantation, Thailand

Amanita ahmadii, a new species of Amanita subgenus Amanitina section Validae from Pakistan

Three New Recorded Species of the Physalacriaceae on Ulleung Island, Korea

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