Four mating-type genes control sexual differentiation in the fission yeast.

Kelly, Mary Margaret; Burke, Julian F.; Smith, Michael D.; Klar, Amar J. S.; Beach, David

doi:10.1002/j.1460-2075.1988.tb02973.x

Cited by 382 publications

(280 citation statements)

References 60 publications

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“…We therefore studied the roles of mating-type genes, in particular mat1-M, in the process of matingtype switching. Of the two genes in the mat1-M cassette, Mi is induced specifically during meiotic induction (10). However, Mc is expressed during vegetative growth (10) and might have a role in the directionality of switching.…”

Section: Resultsmentioning

confidence: 99%

A homolog of male sex-determining factor SRY cooperates with a transposon-derived CENP-B protein to control sex-specific directed recombination

Matsuda

Sugioka-Sugiyama

Mizuguchi

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Schizosaccharomyces pombe cells switch mating type by replacing genetic information at the expressed mat1 locus with sequences copied from mat2-P or mat3-M silent donor loci. The choice of donor locus is dictated by cell type, such that mat2 is the preferred donor in M cells and mat3 is the preferred donor in P cells. Donor choice involves a recombination-promoting complex (RPC) containing Swi2 and Swi5. In P cells, the RPC localizes to a specific DNA element located adjacent to mat3, but in M cells it spreads across the silent mating-type region, including mat2-P. This differential distribution of the RPC regulates nonrandom choice of donors. However, celltype-specific differences in RPC localization are not understood. Here we show that the mat1-M-encoded factor Mc, which shares structural and functional similarities with the male sex-determining factor SRY, is highly enriched at the swi2 and swi5 loci and promotes elevated levels of RPC components. Loss of Mc reduces Swi2 and Swi5 to levels comparable to those in P cells and disrupts RPC spreading across the mat2/3 region. Mc also localizes to loci expressed preferentially in M cells and to retrotransposon LTRs. We demonstrate that Mc localization at LTRs and at swi2 requires Abp1, a homolog of transposon-derived CENP-B protein and that loss of Abp1 impairs Swi2 protein expression and the donor choice mechanism. These results suggest that Mc modulates levels of recombination factors, which is important for mating-type donor selection and for the biased gene conversion observed during meiosis, where M cells serve as preferential donors of genetic information. (1) and mating-type switching in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe (2-5). In these distantly related yeast species, cells alternate between two distinct mating-type alleles by copying genetic information from one of the two silent donor loci to the active mating-type locus via highly orchestrated recombination processes (6-8).The mating-type region of S. pombe contains three linked locimat1, mat2, and mat3-located on chromosome 2. In wild-type homothallic strains, designated h 90 , mat2 is located ∼17 kb centromere-distal to mat1, whereas mat3 is separated from mat2 by an 11-kb interval ( Fig. 1) (8, 9). The mating type of a haploid cell is determined by the presence of P or M information at mat1 (8). Each allele consists of two divergently transcribed genes (10). mat1-P contains the Pc and Pi genes, and mat1-M contains the Mc and Mi genes. mat2 and mat3 contain the same genetic information as mat1-P and mat1-M, respectively, but are maintained in a silent state. These silent mating-type cassettes are embedded in a 20-kb heterochromatin domain surrounded by inverted repeat (IR-R and IR-

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Section: Resultsmentioning

confidence: 99%

A homolog of male sex-determining factor SRY cooperates with a transposon-derived CENP-B protein to control sex-specific directed recombination

Matsuda

Sugioka-Sugiyama

Mizuguchi

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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“…The mat1-M/mat1:3-M cells are unable to undergo meiosis and sporulation as both alleles contain M-information, but the mat1-P/mat1-M heterozygous constitution is essential for inducing sporulation (Kelly et al 1988). However, once mat1-M switch had generated the mat1-P allele, the cell with the resulting mat1-P/mat1:3-M constitution becomes competent to undergo sporulation under nutritional starvation conditions.…”

Section: Possible Modes Of Chromosome 2 Strand Segregation In Successmentioning

confidence: 99%

Unbiased segregation of fission yeast chromosome 2 strands to daughter cells

Klar

Bonaduce

2013

Chromosome Res

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The base complementarity feature (Watson and Crick in Nature 171(4356): [737][738] 1953) and the rule of semi-conservative mode of DNA replication (Messelson and Stahl in Proc Natl Acad Sci U S A 44: [671][672][673][674][675][676][677][678][679][680][681][682] 1958) dictate that two identical replicas of the parental chromosome are produced during replication. In principle, the inherent strand sequence differences could generate nonequivalent daughter chromosome replicas if one of the two strands were epigenetically imprinted during replication to effect silencing/expression of developmentally important genes. Indeed, inheritance of such a strand-and sitespecific imprint confers developmental asymmetry to fission yeast sister cells by a phenomenon called mating/cell-type switching. Curiously, location of DNA strands with respect to each other at the centromere is fixed, and as a result, their selected segregation to specific sister chromatid copies occurs in eukaryotic cells. The yeast system provides a unique opportunity to determine the significance of such biased strand distribution to sister chromatids. We determined whether the cylindrical-shaped yeast cell distributes the specific chromosomal strand to the same cellular pole in successive cycles of cell division. By observing the pattern of recurrent mating-type switching in progenies of individual cells by microscopic analyses, we found that chromosome 2 strands are distributed by the random mode in successive cell divisions. We also exploited unusual "hotspot" recombination features of this system to investigate whether there is selective segregation of strands such that oldest Watsoncontaining strands co-segregate in the diploid cell at mitosis. Our data suggests that chromosome 2 strands are segregated independently to those of the homologous chromosome.

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“…In h + cells, two different genes are expressed from the mat1 locus: mat1-Pc, which controls the sexual differentiation, and mat1-Pm, which controls the initiation of meiosis. Similarly, h -cells express mat1-Mc, which also controls the sexual differentiation, and mat1-Mm, needed to enter meiosis (Kelly et al, 1988).…”

Section: The Pheromone Pathwaymentioning

confidence: 99%

Promoter-driven splicing regulation in fission yeast

Moldón

Malapeira

Gabrielli

et al. 2008

Nature

135

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AGRADECIMIENTOS vi viiDurante toda esta tesis veía clarísimo todo lo que escribir en este apartado y ahora que llega el momento me quedo en blanco... Debe ser la presión de saber que va a ser la sección más leída… Primero agradecer a José y a Elena el poder haber trabajado en este laboratorio durante este tiempo. Han sido casi dos tesis, sí, sí, mi primera página del cuaderno de laboratorio data del 19 de Abril del 2000, todavía recuerdo el primer día, lo primero que hice fue rotular eppendorfs con una temblorosa mano, cuántos nervios. Después de trabajar en muchos temas, momentos difíciles y agradables, odiosos y entrañables, mudanzas, meetings, reuniones, cenas y mil otros recuerdos que me llevo puedo decir que me va a costar estar lejos de este lugar. Un abrazo muy fuerte a los dos!! Mis compañeros de laboratorio, pasados, presentes y futuros, desde aquel chico de la UAB que me enseñó como utilizar una enzima de restricción hasta el chico nuevo que todavía no conozco... No tengo palabras. Habéis sido mi familia todo este tiempo:A la pequeña Ester que me ha cuidado desde el principio como una hermana mayor. A Ana con la que el odio y el cariño se entrelazaban continuamente, muy buena conversadora y con gran ojo crítico. A Carine cuya breve estancia me dejó entrever que hay más de una manera de hacer ciencia. A Jordi (MalaP), compañero de adversidades y aventuras. A Mercè, cuyo apoyo logístico y moral ha sido imprescindible. A Alice, símbolo de la ternura y de la filología italiana, ya se sabe, a caballo donato... A Miriam, una de las mejores personas que conozco, sensata, seria y muy inteligente, una gran científica. A Mónica, maestra de artes marciales y colis. A Blanca, la dulzura personificada y buen público para los chistes. A Nati, también compañera de poyata, penas y alegrías, una post-doc todavía sin título. A Isabel, la catol... una gran amiga y compañera de ChIPs, tranquila que las cosas se van arreglando solas! A Sarela, un meiga pero de las buenas y a Iván (el granaíno), que con su corta pero intensa estancia nos alegró las largas horas de laboratorio. A todos gracias por apoyarme, aguantarme y porque nadie mejor que vosotros sabe lo que supone esta tesis.Al resto de gente de la UPF, los vecinos de laboratorio, especialmente el laboratorio de Señalización Celular (o cariñosamente llamados "los posas") con los que hemos compartido más que un laboratorio, Inmunología (Mari, Bea, Mingui, Julia, Rosa, etc.), grandes amigos dentro y fuera de la UPF, y todos los demás grupos del departamento.Siguiendo la ampliación de espacio, gracias también a toda la gente del PRBB que han ayudado a que estos años se hicieran más amenos. Gracias a ellos se materializó viii la mejor terapia antiestrés, el voley, gracias a los Rabenessen de Caramel (incluido Iván, sí, podrás venir al pica pica de mi tesis, jeje).Gracias a mis compañeros de licenciatura, con ellos inicié este camino y espero seguir sendas cercanas a las suyas.A la gente del laboratorio de Paul Nurse en New York, gracias por haberme hecho pasar una de las m...

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Four mating-type genes control sexual differentiation in the fission yeast.

Cited by 382 publications

References 60 publications

A homolog of male sex-determining factor SRY cooperates with a transposon-derived CENP-B protein to control sex-specific directed recombination

A homolog of male sex-determining factor SRY cooperates with a transposon-derived CENP-B protein to control sex-specific directed recombination

Unbiased segregation of fission yeast chromosome 2 strands to daughter cells

Promoter-driven splicing regulation in fission yeast

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