Four Pristionchus species associated with two mass-occurring Parafontaria laminata populations

Abstract

Phoretic nematodes associated with two mass-occurring populations of the millipede Parafontaria laminata were examined, focusing on Pristionchus spp. The nematodes that propagated on dissected millipedes were genotyped using the D2-D3 expansion segments of the 28S ribosomal RNA gene. Four Pristionchus spp. were detected: P. degawai, P. laevicollis, P. fukushimae, and P. entomophagus. Of the four, P. degawai dominated and it was isolated from more than 90% of the millipedes examined. The haplotypes of partial sequences of mitochondrial cytochrome oxidase subunit I examined for Pristionchus spp. and P. degawai showed high haplotype diversity.

Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea

“…However, similar to cryptic species among plant parasitic nematodes (see Subbotin, 2015 ; Mwamula et al, 2020 ), COI gene sequences are more promising, with significant interspecific variation of 8.3% (51 bp) to 11.7% (72 bp). Thus, in addition to future hybridization studies, the COI gene might be a powerful, discriminating DNA barcoding marker for precise identification of these cryptic species within the genus, despite the existence of haplotypes in some diplogastrid groups, as detailed by Kanzaki et al (2020) .…”