Four glycoside hydrolases are differentially modulated by auxins, cytokinins, abscisic acid and gibberellic acid in apple fruit callus cultures

Alayón-Luaces, Paula; Pagano, Eduardo; Mroginski, L. A.; Sozzi, Gabriel O.

doi:10.1007/s11240-008-9438-1

Cited by 15 publications

(11 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This activity was assumed to belong to ''cell-wall enzymes'', obtained from homogenisation with a saline buffer after successive washings of the cell-wall pellet with water to remove cytosolic proteins. On the other hand, WS-F and NaCl-F a-Xyl activity was hardly noticeable in apple callus cultures (Table 1), as previously stated (Alayón-Luaces et al 2008). Because cell-wall glycosidases may be highly dynamic enzymes responsible for diverse aspects of cell wall properties, we analysed NaCl-F glycosidases with more detail.…”

Section: Resultssupporting

confidence: 65%

“…a-Araf, b-Glc, b-Gal and b-Xyl activity was mainly detected in the NaCl-F as previously described (Alayón-Luaces et al 2008). This activity was assumed to belong to ''cell-wall enzymes'', obtained from homogenisation with a saline buffer after successive washings of the cell-wall pellet with water to remove cytosolic proteins.…”

Section: Resultssupporting

confidence: 54%

“…Primary callus explants were obtained from the enlarged fleshy receptacle. Each explant consisted of a 5-mm apple-tissue cube which was surface sterilized as previously described (Alayón-Luaces et al 2008).…”

Section: Callus Culture and Chemicalsmentioning

confidence: 99%

“…Positive modulation of b-Glc activity by MAN was strong: in MAN-treated calli, WS-F b-Glc activity rose 4.2-(MS0 medium) and 11-fold (MSH medium) in comparison with SUC-treated calli while NaCl-F b-Glc increased 3.14-(MS0 medium) and 2.5-fold (MSH medium) respectively (Table 2). b-Glc may be positively associated with certain stress conditions such as the presence of Picloram and/or PGR starvation (Alayón-Luaces et al 2008). A gene homologous to a b-Glc was found to be expressed in apples exclusively during the infection process of the fruit by Penicillium expansum (Sánchez-Torres and González-Candelas 2003).…”

Section: Effects Of Different Carbohydrates On Glycosyl Hydrolase Actmentioning

confidence: 99%

“…In fact, different problems have hindered research in this area: entry and distribution of PGRs and sugars are uncertain since studies with intact plant organs require vacuum infiltrations, sprays or dips of the organ in different treatment solutions and each organ requires a singular treatment depending on its anatomy and morphology. Previous reports proved that apple callus tissue culture is an artificial biological system providing a convenient easy-to-handle source of plant material, which can be rapidly regenerated, and from which cell-wall enzymes may be obtained without difficulty (Alayón-Luaces et al 2008;Liu et al 2006). Since callus cultures can continue to grow in the basal medium with no plant growth regulators (PGRs) due to the so-called ''habituation'' phenomenon (Alayón-Luaces et al 2008), this system allows comprehensive studies involving the addition of different carbohydrate types and/or concentrations with or without any addition of PGRs at physiological levels.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Activity levels of six glycoside hydrolases in apple fruit callus cultures depend on the type and concentration of carbohydrates supplied and the presence of plant growth regulators

Alayón-Luaces

Pagano

Mroginski

et al. 2009

Plant Cell Tiss Organ Cult

Self Cite

View full text Add to dashboard Cite

Sucrose presence and concentration modulated in different ways and to different extents the activity of six plant glycoside hydrolases (PGHs) extracted from apple callus cultures, both in the water soluble fraction (WS-F) and in the NaCl-released fraction (NaCl-F). b-D-Glucosidase activity increased because of sucrose starvation and the addition of sucrose decreased both WS-F and NaCl-F b-D-glucosidase from calli grown in a Murashige and Skoog's basal medium with (MSH) or without (MS0) plant growth regulators (PGRs). WS-F and NaCl-F a-L-arabinofuranosidase, NaCl-F b-D-galactosidase and NaCl-F b-Dxylosidase activity reached a maximum when 0.045 M sucrose was added to the MS0 medium with an ensuing decline at higher sucrose concentrations. a-D-Galactosidase and a-D-xylosidase activity reached a maximum when 0.045 M sucrose was supplied and did not decline significantly in 0.09 M sucrose-supplied calli. When the effects of PGR presence or absence were analysed, NaCl-F b-Dglucosidase, a-D-galactosidase, b-D-galactosidase, a-D-xylosidase and b-D-xylosidase activities were found to be higher in MS0 than in MSH. To assess whether sugar effects were sucrose-specific, other sugars (glucose, fructose, galactose, maltose, lactose, raffinose, sorbitol and mannitol) were tested, with or without PGR supplementation. In general, sugar alcohols (mannitol, sorbitol) and some monosaccharides (fructose and glucose in particular) were better inducers of NaCl-F a-L-arabinofuranosidase, b-D-galactosidase and b-D-xylosidase activity than disaccharides (sucrose, maltose, and lactose) or the trisaccharide raffinose. This trend was not widespread to all PGHs assessed since sucrose-supplemented calli displayed higher NaCl-F a-D-galactosidase than those supplemented with glucose, galactose, sorbitol or mannitol. These results show that sugars supplied to callus tissue cultures as a carbon source can also modulate PGH activity. Modulation is different for each PGH, sugar-specific and, at least in the case of sucrose, concentration-dependent. Results also suggest the existence of regulatory interactions between PGRs and sugars as part of an intricate sensing and signalling network. Combination of PGR, sugar type and concentration should be taken into account to maximize each PGH activity for further enzyme studies.Keywords Malus 9 domestica Á a-L-Arabinofuranosidase Á a-and b-D-Galactosidase Á a-and b-D-Xylosidase Á b-D-Glucosidase Á Sugars

show abstract

Section: Resultssupporting

confidence: 65%

Section: Resultssupporting

confidence: 54%

Section: Callus Culture and Chemicalsmentioning

confidence: 99%

Section: Effects Of Different Carbohydrates On Glycosyl Hydrolase Actmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Activity levels of six glycoside hydrolases in apple fruit callus cultures depend on the type and concentration of carbohydrates supplied and the presence of plant growth regulators

Alayón-Luaces

Pagano

Mroginski

et al. 2009

Plant Cell Tiss Organ Cult

Self Cite

View full text Add to dashboard Cite

show abstract

In vitro tissue culture of apple and other Malus species: recent advances and applications

Silva

Gulyás

Magyar-Tábori

et al. 2019

Planta

View full text Add to dashboard Cite

Main conclusion Studies on the tissue culture of apple have allowed for molecular, biotechnological and applied breeding research to advance. In the past 8 years, over 100 papers advancing basic biology, genetic transformation and cryobiology have emerged. Apple (Malus × domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. In vitro tissue culture is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This updated review presents a synthesis of findings related to the tissue culture of apple and other Malus spp. between 2010 and 2018. Increasingly complex molecular studies that are examining the apple genome, for example, in a bid to identify the cause of epigenetic mutations and the role of transposable elements in this process would benefit from genetically stable source material, which can be produced in vitro. Several notable or curious in vitro culture methods have been reported to improve shoot regeneration and induce the production of tetraploids in apple cultivars and rootstocks. Existing studies have revealed the molecular mechanism underlying the inhibition of adventitious roots by cytokinin. The use of the plant growth correction factor allows hypothetical shoot production from leaf-derived thin cell layers relative to conventional leaf explants to be determined. This updated review will allow novices and established researchers to advance apple and Malus biotechnology and breeding programs.

show abstract

Reliable callus-induced plantlet regeneration from leaf explants of Lagerstroemia speciosa and genetic fidelity assessment through ISSR markers

Wu,

Zhang,

Dixon

et al. 2024

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Four glycoside hydrolases are differentially modulated by auxins, cytokinins, abscisic acid and gibberellic acid in apple fruit callus cultures

Abstract: a-L-Arabinofuranosidase, a-and b-D-xy

Cited by 15 publications

References 29 publications

Activity levels of six glycoside hydrolases in apple fruit callus cultures depend on the type and concentration of carbohydrates supplied and the presence of plant growth regulators

Activity levels of six glycoside hydrolases in apple fruit callus cultures depend on the type and concentration of carbohydrates supplied and the presence of plant growth regulators

In vitro tissue culture of apple and other Malus species: recent advances and applications

Reliable callus-induced plantlet regeneration from leaf explants of Lagerstroemia speciosa and genetic fidelity assessment through ISSR markers

Contact Info

Product

Resources

About